两个黑麦草品种对镉吸收富集比较研究
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摘要
采用盆栽试验研究比较2个黑麦草品种在不同Cd污染水平(0、75、150、300、600 mg/kg)下生长量、抗氧化酶活性、Cd含量及积累量的差异,以筛选出更适合作为土壤镉污染修复的品种。结果表明:随着土壤镉水平的增加,2个黑麦草品种根系干重、‘阿伯德’地上部干重和植株总干重呈先增加后降低的趋势。土壤镉污染水平为75 mg/kg时‘,阿伯德’地上部和植株总干重达到最大值(10.92 g/盆和12.03 g/盆),较对照分别增加了11.13%和10.67%。2个黑麦草品种各部位SOD活性、CAT活性和根系POD活性,均随土壤镉水平的增加呈先升高后降低的趋势,地上部MDA含量则逐渐升高。2个黑麦草品种镉转运系数、镉富集系数均大于1。黑麦草对Cd耐性和吸收富集存在基因型差异。比较2个黑麦草品种,在75～600 mg/kg Cd胁迫下,‘阿伯德’品种地上部干重和总干重均高于‘邦德’品种,且‘阿伯德’地上部和根系镉含量及镉积累量高于‘邦德’‘。阿伯德’更适合作为土壤镉污染的植物修复材料。
Pot experiments were conducted to compare the difference of biomass, activity of antioxidant enzymes, Cd concentration and accumulation of two ryegrass varieties under different Cd levels(0, 75, 150, 300 and 600 mg/kg), to select suitable variety for soil cadmium pollution remediation. The results showed that the dry weight of roots of the two ryegrass varieties, the dry weight of the overground part and total plant dry weight of‘Abode'increased first and then decreased with the increase of Cd level. The dry weight of the overground part and total plant dry weight of‘Abode'reached the maximum value of 10.92 g/pot and 12.03 g/pot under 75 mg/kg Cd, increased by 11.13% and 10.67% respectively compared with those of the control. The activities of SOD and CAT in shoots and roots, and the activities of POD in roots of the two ryegrass varieties increased first and then decreased with the increase of Cd level. The content of MDA in shoots increased with the increase of Cd level. The Cd transport coefficient and Cd enrichment coefficient of the two ryegrass varieties were higher than 1. There were genotypic differences in Cd tolerance, absorption and accumulation of different ryegrass varieties. The dry weight of shoots and plants of‘Abode'were higher than that of‘Bond', and the content and accumulation of Cd in shoots and roots of‘Abode'were higher than that of‘Bond'under 75-600 mg/kg Cd stress. Therefore,‘Abode'is more suitable as a phytoremediation material for soil cadmium pollution.
Pot experiments were conducted to compare the difference of biomass, activity of antioxidant enzymes, Cd concentration and accumulation of two ryegrass varieties under different Cd levels(0, 75, 150, 300 and 600 mg/kg), to select suitable variety for soil cadmium pollution remediation. The results showed that the dry weight of roots of the two ryegrass varieties, the dry weight of the overground part and total plant dry weight of‘Abode'increased first and then decreased with the increase of Cd level. The dry weight of the overground part and total plant dry weight of‘Abode'reached the maximum value of 10.92 g/pot and 12.03 g/pot under 75 mg/kg Cd, increased by 11.13% and 10.67% respectively compared with those of the control. The activities of SOD and CAT in shoots and roots, and the activities of POD in roots of the two ryegrass varieties increased first and then decreased with the increase of Cd level. The content of MDA in shoots increased with the increase of Cd level. The Cd transport coefficient and Cd enrichment coefficient of the two ryegrass varieties were higher than 1. There were genotypic differences in Cd tolerance, absorption and accumulation of different ryegrass varieties. The dry weight of shoots and plants of‘Abode'were higher than that of‘Bond', and the content and accumulation of Cd in shoots and roots of‘Abode'were higher than that of‘Bond'under 75-600 mg/kg Cd stress. Therefore,‘Abode'is more suitable as a phytoremediation material for soil cadmium pollution.
引文
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[14]唐让云,曹靖,董放,等.淋洗与植物作用耦合对盐渍化土壤的改良效应[J].生态学报,2015,35(17):5686-5694.
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[18]李志贤,陈章,陈国梁,等.镉富集植物油菜与玉米间作对玉米吸收积累镉的影响[J].生态学杂志,2016,35(1):26-31.
[19]吴福忠,杨万勤,张健,等.镉胁迫对桂花生长和养分积累,分配与利用的影响[J].植物生态学报,2010,34(10):1220-1226.
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[21]施宠,王纯利,黄长福,等.镉胁迫对野燕麦幼苗生长及其生理特性的影响[J].草地学报,2015,23(3):526-532.
[22]韩航,陈雪娇,侯晓龙,等.镉胁迫对类芦生长及酶活性的影响[J].农业环境科学学报,2016,35(4):647-653.
[23]陈永勤.镉富集植物镉积累基因型差异及分子机理研究[D].重庆:西南大学,2017.
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[25]孙园园.耐镉植物抗性及富集规律的研究[D].贵阳:贵州大学,2015.
[26]王全辉,梁雪,董元杰,等.外源NO对镉胁迫下黑麦草生长的缓解效应[J].中国草地学报,2012,34(2):30-35.
[27]Liu D L,Zhang S P,Chen Z,et al.Soil cadmium regulates antioxidases in sorghum[J].Agricultural Sciences in China,2010,9(10):1475-1480.
[28]牛文婷.木炭对NaCl胁迫下小麦根系蛋白质组影响的研究[D].太原:山西师范大学,2016.
[29]孟利娟.一氧化氮和海藻糖对白灵侧耳高温响应抗氧化途径的影响研究[D].北京:中国农业科学院,2015.
[30]夏民旋,王维,袁瑞,等.超氧化物歧化酶与植物抗逆性[J].分子植物育种,2015,13(11):2633-2646.
[31]李冬琴,陈桂葵,郑海,等.镉对两品种玉豆生长和抗氧化酶的影响[J].农业环境科学学报,2015,34(2):221-226.
[32]张凯凯,孙圆圆,徐玲玲,等.镉胁迫对一年生黑麦草生理生化特性的影响[J].山地农业生物学报,2016,35(1):86-89.
[33]米艳华,雷梅,黎其万,等.滇南矿区重金属污染耕地的植物修复及其健康风险[J].生态环境学报,2016,25(5):864-871
[34]陈璐,米艳华,林昕,等.土壤-三七系统重金属污染调查及相关分析[J].中国中药杂志,2014,39(14):2608.
[35]冯鹏,孙力,申晓慧,等.多年生黑麦草对Pb、Cd胁迫的响应及富集能力研究[J].草业学报,2016,25(1):153-162.
[2]江玲.黑麦草、丛枝菌根对不同品种番茄镉吸收、富集的影响[D].重庆:西南大学,2015.
[3]郑莹辉,熊仕娟,徐卫红,等.纳米沸石对大白菜镉吸收及土壤有效镉含量的影响[J].农业环境科学学报,2016,35(12):2353-2360.
[4]秦余丽,熊仕娟,徐卫红,等.纳米沸石对大白菜生长、抗氧化酶活性及镉形态、含量的影响[J].环境科学,2017,38(3):1189-1200.
[5]周坤.外源锌、铁对番茄镉积累的影响研究[D].重庆:西南大学,2014.
[6]Li J Y,Liu J,Dong D,et al.Natural variation underlies alterations in Nramp aluminum transporter(NRAT1)expression and function that play a key role in rice aluminum tolerance[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(17):6503-6508.
[7]韦妮玉,张新英.桂西北矿区农田土壤镉污染评价[J].绿色科技,2016(18):31-33.
[8]杨启良,武振中,陈金陵,等.植物修复重金属污染土壤的研究现状及其水肥调控技术展望[J].生态环境学报,2015(6):1075-1084.
[9]李松克,张春林,李克勤,等.多年生黑麦草对黄壤重金属污染的修复[J].贵州农业科学,2014,42(11):147-151.
[10]孙园园,关萍,何杉,等.镉胁迫对多花黑麦草镉积累特征、生理抗性及超微结构的影响[J].草业科学,2016,33(8):1589-1597.
[11]李希铭.草本植物对镉的耐性和富集特征研究[D].北京:北京林业大学,2016.
[12]魏树强.多年生黑麦草(Lolium perene L.)耐镉机理与LpGCS基因的克隆和功能分析[D].北京:中国林业科学研究院,2014.
[13]黄登峰,席嘉宾,赵运林.镉胁迫下两个多年生黑麦草品种的生理响应[J].北方园艺,2016(3):66-68.
[14]唐让云,曹靖,董放,等.淋洗与植物作用耦合对盐渍化土壤的改良效应[J].生态学报,2015,35(17):5686-5694.
[15]Liu Z,He Liu Z L,He X Y,et al.Accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator:Lonicera japonica Thunb[J].Journal of Hazardous Materials,2009,169(1):170-175.
[16]张志良.植物生理学实验指导(第二版)[M].北京:高等教育出版社,1990.
[17]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[18]李志贤,陈章,陈国梁,等.镉富集植物油菜与玉米间作对玉米吸收积累镉的影响[J].生态学杂志,2016,35(1):26-31.
[19]吴福忠,杨万勤,张健,等.镉胁迫对桂花生长和养分积累,分配与利用的影响[J].植物生态学报,2010,34(10):1220-1226.
[20]张兴梅,杨清伟,李扬.土壤镉污染现状及修复研究进展[J].河北农业科学,2010,03:79-81.
[21]施宠,王纯利,黄长福,等.镉胁迫对野燕麦幼苗生长及其生理特性的影响[J].草地学报,2015,23(3):526-532.
[22]韩航,陈雪娇,侯晓龙,等.镉胁迫对类芦生长及酶活性的影响[J].农业环境科学学报,2016,35(4):647-653.
[23]陈永勤.镉富集植物镉积累基因型差异及分子机理研究[D].重庆:西南大学,2017.
[24]李慧芳,王瑜,袁庆华.镉胁迫对多年生黑麦草生长及生理特性的影响[J].中国草地学报,2014,36(4):79-84.
[25]孙园园.耐镉植物抗性及富集规律的研究[D].贵阳:贵州大学,2015.
[26]王全辉,梁雪,董元杰,等.外源NO对镉胁迫下黑麦草生长的缓解效应[J].中国草地学报,2012,34(2):30-35.
[27]Liu D L,Zhang S P,Chen Z,et al.Soil cadmium regulates antioxidases in sorghum[J].Agricultural Sciences in China,2010,9(10):1475-1480.
[28]牛文婷.木炭对NaCl胁迫下小麦根系蛋白质组影响的研究[D].太原:山西师范大学,2016.
[29]孟利娟.一氧化氮和海藻糖对白灵侧耳高温响应抗氧化途径的影响研究[D].北京:中国农业科学院,2015.
[30]夏民旋,王维,袁瑞,等.超氧化物歧化酶与植物抗逆性[J].分子植物育种,2015,13(11):2633-2646.
[31]李冬琴,陈桂葵,郑海,等.镉对两品种玉豆生长和抗氧化酶的影响[J].农业环境科学学报,2015,34(2):221-226.
[32]张凯凯,孙圆圆,徐玲玲,等.镉胁迫对一年生黑麦草生理生化特性的影响[J].山地农业生物学报,2016,35(1):86-89.
[33]米艳华,雷梅,黎其万,等.滇南矿区重金属污染耕地的植物修复及其健康风险[J].生态环境学报,2016,25(5):864-871
[34]陈璐,米艳华,林昕,等.土壤-三七系统重金属污染调查及相关分析[J].中国中药杂志,2014,39(14):2608.
[35]冯鹏,孙力,申晓慧,等.多年生黑麦草对Pb、Cd胁迫的响应及富集能力研究[J].草业学报,2016,25(1):153-162.
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