打顶后烟叶内源生长素含量的变化对绿原酸含量的影响
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摘要
【目的】为了研究打顶后烟叶中生长素(IAA)含量对绿原酸合成的影响。【方法】以红花大金元为试验材料,盆栽试验采用完全随机设计,利用高效液相色谱法和酶联免疫法分析烟叶IAA含量、绿原酸含量以及绿原酸合成与降解关键酶活性。【结果】苗期打顶后烟叶中内源IAA和绿原酸含量均降低;现蕾后打顶烟叶中IAA和绿原酸含量在第3天后趋于下降。通过NAA(α-Naphthalene acetic acid)和NPA(N-1-naphthylphthalamic acid)回补试验发现,打顶处理和NPA处理烟叶中内源IAA和绿原酸含量降低,过氧化物酶(POD)和多酚氧化酶(PPO)活性升高;打顶+NAA处理烟叶中内源IAA和绿原酸含量升高,POD和PPO活性降低。与未打顶处理(对照)相比,其他几个处理烟叶中苯丙氨酸解氨酶(PAL)、4-香豆酸连接酶(4CL)、肉桂酸4-羟化酶(C4H)、对-香豆酸3′-羟化酶(C3H)和羟基肉桂酰辅酶A:莽草酸/奎尼酸羟基肉桂酰转移酶(HQT)活性升高。【结论】打顶后烟叶中绿原酸含量与内源生长素含量变化密切相关,并受POD和PPO活性调控。
【Objective】The present paper was conducted to study the effect of auxin content on chlorogenic acid synthesis in tobacco leaves after topping.【Method】Honghuadajinyuan taken as experimental materials, a pot experiment was carried out with complete random designs. The content of IAA and chlorogenic acid were analyzed by high performance liquid chromatography, the activity of chlorogenic acid synthesis and degradation enzymes were by ELISA.【Result】At the seedling stage endogenous IAA content decreased and chlorogenic acid content decreased after topping, while topping after the flower-bud appeared the IAA content of the tobacco leaves decreased after the third day, and the chlorogenic acid has the same changing rules. According to the NAA and NPA replenishment tests, the content of endogenous IAA in topping plants and NPA-treated tobacco leaves was decreased, the POD and PPO activities were increased, and the chlorogenic acid content was decreased;topping+NAA treatment elevated the content of chlorogenic acid and endogenous IAA in tobacco leaves, decreased POD and PPO the activity increased. The activity of PAL, 4CL, C4H, C3H and HQT increased in comparison with the untopping treatment.【Conclusion】The content of chlorogenic acid was closely related to the content of endogenous auxin in tobacco leaves after topping, and regulated by the activity of POD and PPO.
【Objective】The present paper was conducted to study the effect of auxin content on chlorogenic acid synthesis in tobacco leaves after topping.【Method】Honghuadajinyuan taken as experimental materials, a pot experiment was carried out with complete random designs. The content of IAA and chlorogenic acid were analyzed by high performance liquid chromatography, the activity of chlorogenic acid synthesis and degradation enzymes were by ELISA.【Result】At the seedling stage endogenous IAA content decreased and chlorogenic acid content decreased after topping, while topping after the flower-bud appeared the IAA content of the tobacco leaves decreased after the third day, and the chlorogenic acid has the same changing rules. According to the NAA and NPA replenishment tests, the content of endogenous IAA in topping plants and NPA-treated tobacco leaves was decreased, the POD and PPO activities were increased, and the chlorogenic acid content was decreased;topping+NAA treatment elevated the content of chlorogenic acid and endogenous IAA in tobacco leaves, decreased POD and PPO the activity increased. The activity of PAL, 4CL, C4H, C3H and HQT increased in comparison with the untopping treatment.【Conclusion】The content of chlorogenic acid was closely related to the content of endogenous auxin in tobacco leaves after topping, and regulated by the activity of POD and PPO.
引文
[1]王柱石,王铮,范文彪,等.打顶时期与有效留叶数对烤烟产量及质量的影响[J].贵州农业科学,2017,45(1):39-43.
[2]李力,杨涓,戴亚,等.烤烟中绿原酸、莨菪亭和芸香苷的分布研究[J].中国烟草学报,2008,14(4):13-17.
[3]杨银菊,张彦,王树声,等.打顶对烟草叶片多酚代谢及其关键酶的影响[J].中国烟草学报,2018,24(1):60-67.
[4]彭东.光质、光强对烤烟苯丙烷代谢关键酶及多酚产物的影响[D].北京:中国农业科学院,2013.
[5]袁卫瑜,王兴德,李军营,等.烤烟生长过程中多酚类物质及其相关酶活性的变化[J].西南农业学报,2017,30(7):1528-1532.
[6]江豪,陈朝阳.打顶、留叶对K326烟叶产量及质量的影响[J].福建农林大学学报(自然版),2001,30(3):329-333.
[7]葛龙,易克,韩定国,等.打顶留叶数对烤烟品质的影响[J].湖南农业科学,2009(8):21-23.
[8]郭丽豚,张福锁.打顶对烟草生长、钾素吸收及其分配的影响[J].应用生态学报,2002,13(7):819-822.
[9]代晓燕,苏以荣,魏文学,等.打顶对烤烟植株钾素代谢和钾离子通道基因表达的影响[J].中国农业科学,2009,42(3):854-861.
[10]张婷,汪宏毅,王国宏,等.顶端调控措施对烤烟叶片多酚氧化酶活性和酚类化合物含量的影响[J].安徽农业科学,2007(36):11875-11876.
[11]田效园,韩锦峰,刘华山.外源物质处理对烟叶内源激素含量的影响[J].安徽农业科学,2016(8):26-27.
[12]时向东,刘艳芳,文志强,等.打顶对烟株根系活力及内源激素含量的影响[J].西北农林科技大学学报:自然科学版,2008,36(2):143-147.
[13]Irisarri P,Zhebentyayeva T,Errea P,et al.Differential expression of phenylalanine ammonia lyase (PAL ) genes implies distinct roles in development of graft incompatibility symptoms in Prunus [J].Scientia Horticulturae,2016,204:16-24.
[14]Reichert A I,He X Z,Dixon R A.Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum):characterization of the four tobacco PAL genes and active heterotetrameric enzymes[J].Biochemical Journal,2009,424(2):233-242.
[15]Hoffmann L,Besseau S,Geoffroy P,et al.Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis[J].The Plant Cell,2004,16(6):1446-1465.
[16]Comino C,Lanteri S,Portis E,et al.Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L[J].BMC Plant Biology,2007,7(1):1-14.
[17]蒋向辉.金银花CGA合成途径关键酶基因克隆与功能分析[D].长沙:湖南大学,2013.
[18]李洋,李明,岳玮,等.烟草 NtHCT 基因对次生代谢物质绿原酸和类黄酮合成的影响[J].中国烟草学报,2015,21(6):127-131.
[19]王丽,王万兴,李小波,等.植物中多酚氧化酶的研究进展[C]// 马铃薯产业与精准扶贫,2017.
[20]Jia H,Zhao P,Wang B,et al.Overexpression of Polyphenol Oxidase Gene in Strawberry Fruit Delays the Fungus Infection Process[J].Plant Molecular Biology Reporter,2016,34(3):592-606.
[21]Constabel C P,Bergey D R,Ryan C A.Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway[J].Proceedings of the National Academy of Sciences of the United States of America,1995,92(2):407-411.
[22]黄勇,周冀衡,李鹏飞,等.打顶对烤烟主要化学成分及多酚氧化酶活性的影响[J].作物研究,2009,23(3):188-190.
[23]Shi Q,Li C,Zhang F.Nicotine synthesis in Nicotiana tabacum L.induced by mechanical wounding is regulated by auxin[J].Journal of Experimental Botany,2006,57(11):2899-2907.
[24]文颖强,冯嘉玥,万春雁.葡萄组织培养苗不同节位茎段的生长、生根以及多酚氧化酶和IAA氧化酶的活性[J].植物生理学通讯,2007(4):723-724.
[2]李力,杨涓,戴亚,等.烤烟中绿原酸、莨菪亭和芸香苷的分布研究[J].中国烟草学报,2008,14(4):13-17.
[3]杨银菊,张彦,王树声,等.打顶对烟草叶片多酚代谢及其关键酶的影响[J].中国烟草学报,2018,24(1):60-67.
[4]彭东.光质、光强对烤烟苯丙烷代谢关键酶及多酚产物的影响[D].北京:中国农业科学院,2013.
[5]袁卫瑜,王兴德,李军营,等.烤烟生长过程中多酚类物质及其相关酶活性的变化[J].西南农业学报,2017,30(7):1528-1532.
[6]江豪,陈朝阳.打顶、留叶对K326烟叶产量及质量的影响[J].福建农林大学学报(自然版),2001,30(3):329-333.
[7]葛龙,易克,韩定国,等.打顶留叶数对烤烟品质的影响[J].湖南农业科学,2009(8):21-23.
[8]郭丽豚,张福锁.打顶对烟草生长、钾素吸收及其分配的影响[J].应用生态学报,2002,13(7):819-822.
[9]代晓燕,苏以荣,魏文学,等.打顶对烤烟植株钾素代谢和钾离子通道基因表达的影响[J].中国农业科学,2009,42(3):854-861.
[10]张婷,汪宏毅,王国宏,等.顶端调控措施对烤烟叶片多酚氧化酶活性和酚类化合物含量的影响[J].安徽农业科学,2007(36):11875-11876.
[11]田效园,韩锦峰,刘华山.外源物质处理对烟叶内源激素含量的影响[J].安徽农业科学,2016(8):26-27.
[12]时向东,刘艳芳,文志强,等.打顶对烟株根系活力及内源激素含量的影响[J].西北农林科技大学学报:自然科学版,2008,36(2):143-147.
[13]Irisarri P,Zhebentyayeva T,Errea P,et al.Differential expression of phenylalanine ammonia lyase (PAL ) genes implies distinct roles in development of graft incompatibility symptoms in Prunus [J].Scientia Horticulturae,2016,204:16-24.
[14]Reichert A I,He X Z,Dixon R A.Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum):characterization of the four tobacco PAL genes and active heterotetrameric enzymes[J].Biochemical Journal,2009,424(2):233-242.
[15]Hoffmann L,Besseau S,Geoffroy P,et al.Silencing of hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase affects phenylpropanoid biosynthesis[J].The Plant Cell,2004,16(6):1446-1465.
[16]Comino C,Lanteri S,Portis E,et al.Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L[J].BMC Plant Biology,2007,7(1):1-14.
[17]蒋向辉.金银花CGA合成途径关键酶基因克隆与功能分析[D].长沙:湖南大学,2013.
[18]李洋,李明,岳玮,等.烟草 NtHCT 基因对次生代谢物质绿原酸和类黄酮合成的影响[J].中国烟草学报,2015,21(6):127-131.
[19]王丽,王万兴,李小波,等.植物中多酚氧化酶的研究进展[C]// 马铃薯产业与精准扶贫,2017.
[20]Jia H,Zhao P,Wang B,et al.Overexpression of Polyphenol Oxidase Gene in Strawberry Fruit Delays the Fungus Infection Process[J].Plant Molecular Biology Reporter,2016,34(3):592-606.
[21]Constabel C P,Bergey D R,Ryan C A.Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway[J].Proceedings of the National Academy of Sciences of the United States of America,1995,92(2):407-411.
[22]黄勇,周冀衡,李鹏飞,等.打顶对烤烟主要化学成分及多酚氧化酶活性的影响[J].作物研究,2009,23(3):188-190.
[23]Shi Q,Li C,Zhang F.Nicotine synthesis in Nicotiana tabacum L.induced by mechanical wounding is regulated by auxin[J].Journal of Experimental Botany,2006,57(11):2899-2907.
[24]文颖强,冯嘉玥,万春雁.葡萄组织培养苗不同节位茎段的生长、生根以及多酚氧化酶和IAA氧化酶的活性[J].植物生理学通讯,2007(4):723-724.
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