IKnife-REIMS联用技术对南极犬牙鱼脂质组学轮廓检测
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摘要
基于IKnife-快速蒸发离子化质谱技术对低脂鱼类南极犬牙鱼的肌肉组织进行脂质组学轮廓检测,并通过响应面分析优化系统参数。结果表明,最优参数为输出功率25 W、切割速率0.50 mm/s、切割长度1.0 cm。在最佳实验条件下共检出脂肪酸离子17种,其中信号响应强度最大为m/z 327.23,相对含量达到了17.81%,经鉴定为脂肪酸(fatty acid,FA),结构为FA 22∶6,其次分别为m/z 255.23(FA 16∶0,9.81%)和m/z 281.25(FA 18∶1,9.09%);检出磷脂离子10种,质量范围为m/z 736.49～909.55,信号最强离子峰m/z 885.55经鉴定为磷脂酰肌醇(phosphatidylinositol,PI)离子[PI 38∶4-H]-,相对丰度达到了19.30%,其次为磷脂酰乙醇胺(phosphatidylethanolamine,PE)离子m/z 790.54([PE 40∶6-H]-,15.24%)。方法验证结果显示,目标离子的信噪比为35.4～95.3,偏差为4.17×10~(-6)～9.78×10~(-6),日内精密度相对标准偏差为3.7%～5.6%,日间精密度相对标准偏差为5.9%～7.3%。本实验方法灵敏度和分辨率高、检测速度快、结果稳定,为脂质组学研究和鱼类生物信息检测提供了新的技术手段。
Rapid evaporative ionization mass spectrometry(REIMS) was employed for lipidomic profiling of Patagonian toothfish, a kind of low-fat fish. The instrumental parameters were optimized using response surface methodology with a Box-Behnken design as follows: power output 25 W, cutting speed was 0.50 mm/s, and cutting length 1.0 cm. Under these optimized conditions, a total of 17 fatty acid molecular species were detected, among which the signal intensity of m/z 327.23(FA 22:6, 17.81%) was the highest, followed by m/z 255.23(FA 16:0, 9.81%), and m/z 281.25(FA 18:1, 9.09%).A total of 10 phospholipid molecular species were tentatively identified in the m/z range from 736.49 to 909.55. The peak at m/z 885.55 was the most intensive with a relative content of 19.30%, which was identified as phosphatidylinositol(PI)species [PI 38:4–H]–, followed by phosphatidylethanolamine(PE) species at m/z 790.54([PE 40:6–H]–, 15.24%). The method was validated and the results showed that the signal-to-noise ratios of the target ions were in the range of 35.4–95.3,with error values of 4.17 × 10~(-6)–9.78 × 10~(-6). The relative standard deviations(RSDs) of intra-day and inter-day precision were 3.7%–5.6% and 5.9%–7.3%, respectively. The results indicate that this method is sensitive and stable with high resolution. It provides a totally new technology for lipidomic and bioinformatics analysis offish.
Rapid evaporative ionization mass spectrometry(REIMS) was employed for lipidomic profiling of Patagonian toothfish, a kind of low-fat fish. The instrumental parameters were optimized using response surface methodology with a Box-Behnken design as follows: power output 25 W, cutting speed was 0.50 mm/s, and cutting length 1.0 cm. Under these optimized conditions, a total of 17 fatty acid molecular species were detected, among which the signal intensity of m/z 327.23(FA 22:6, 17.81%) was the highest, followed by m/z 255.23(FA 16:0, 9.81%), and m/z 281.25(FA 18:1, 9.09%).A total of 10 phospholipid molecular species were tentatively identified in the m/z range from 736.49 to 909.55. The peak at m/z 885.55 was the most intensive with a relative content of 19.30%, which was identified as phosphatidylinositol(PI)species [PI 38:4–H]–, followed by phosphatidylethanolamine(PE) species at m/z 790.54([PE 40:6–H]–, 15.24%). The method was validated and the results showed that the signal-to-noise ratios of the target ions were in the range of 35.4–95.3,with error values of 4.17 × 10~(-6)–9.78 × 10~(-6). The relative standard deviations(RSDs) of intra-day and inter-day precision were 3.7%–5.6% and 5.9%–7.3%, respectively. The results indicate that this method is sensitive and stable with high resolution. It provides a totally new technology for lipidomic and bioinformatics analysis offish.
引文
[1]缪圣赐,邱卫华,周雨思,等.南极周边海域犬牙鱼资源及其渔业概况[J].渔业信息与战略,2015,30(1):61-65.DOI:10.13233/j.cnki.fishis.2015.01.011.
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[12]SHEN Q,CHEUNG H Y.TiO2/SiO2 core-shell composite-based sample preparation method for selective extraction of phospholipids from shrimp waste followed by hydrophilic interaction chromatography coupled with quadrupole time-of-flight/mass spectrometry analysis[J].Journal of Agricultural and Food Chemistry,2014,62(36):8944-8951.DOI:10.1021/jf503040p.
[13]STRITTMATTER N,REBEC M,JONES E A,et al.Characterization and identification of clinically relevant microorganisms using rapid evaporative ionization mass spectrometry[J].Analytical Chemistry,2014,86(13):6555-6562.DOI:10.1021/ac501075f.
[14]SCH?FER K C,DéNES J,ALBRECHT K,et al.In vivo,in situ tissue analysis using rapid evaporative ionization mass spectrometry[J].Angewandte Chemie,2010,48(44):8240-8242.DOI:10.1002/anie.200902546.
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[16]LIN Y N,WANG H X,RAO W,et al.Rapid evaporative ionization mass spectrometry based lipidomics tracking of grass carp(Ctenopharyngodon idellus)during in vitro multiple-stage digestion[J].Journal of Agricultural and Food Chemistry,2018,66(24):6246-6253.DOI:10.1021/acs.jafc.8b01644.
[17]VERPLANKEN K,STEAD S,JANDOVA R,et al.Rapid evaporative ionization mass spectrometry for high-throughput screening in food analysis:the case of boar taint[J].Talanta,2017,169:30-36.DOI:10.1016/j.talanta.2017.03.056.
[18]BALOG J,SASI-SZABO,L,KINROSS J,et al.Intraoperative tissue identification using rapid evaporative ionization mass spectrometry[J].Science Translational Medicine,2013,194:153-154.DOI:10.1126/scitranslmed.3005623.
[19]缪圣赐,邱卫华,周雨思,等.南极周边海域犬牙鱼资源及其渔业概况[J].渔业信息与战略,2015,30(1):61-65.DOI:10.13233/j.cnki.fishis.2015.01.011.
[20]SHEN Q,WANG Y,GONG L,et al.Shotgun lipidomics strategy for fast analysis of phospholipids in fisheries waste and its potential in species differentiation[J].Journal of Agricultural and Food Chemistry,2012,60(37):9384-9393.DOI:10.1021/jf303181s.
[21]XU P P,WANG Y,CHEN J J,et al.Lipidomic profiling of juvenile yellow head catfish(Pelteobagrus fulvidraco)in response to fucoidan diet[J].Aquaculture International,2017,25(3):1123-1143.DOI:10.1007/s1049.
[22]LIN Y,WANG H,RAO W,et al.Structural characteristics of dietary fiber(Vigna radiata L.Hull)and its inhibitory effect on phospholipid digestion revealed by novel rapid evaporative ionization mass spectrometry based lipidomics[J].Food Control,2019,98:74-81.DOI:10.1016/j.foodcont.2018.11.016.
[23]BALOG J,PERENYI D,GUALLARHOYAS C,et al.Identification of the species of origin for meat products by rapid evaporative ionization mass spectrometry[J].Journal of Agricultural and Food Chemistry,2016,64(23):4793-4800.DOI:10.1021/acs.jafc.6b01041.
[24]柯庆青,李诗言,周凡,等.基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素[J].食品科学,2017,38(24):241-246.DOI:10.7506/spkx1002-6630-201724039.
[2]BRIGDEN K E,MARSHALL C T,SCOTT B E,et al.Interannual variability in reproductive traits of the Patagonian toothfish Dissostichus eleginoides around the sub-Antarctic island of South Georgia[J].Journal of Fish Biology,2017,91(1):278-301.DOI:10.1111/jfb.13344.
[3]唐建业.南极海洋生物资源养护委员会与中国:第30届年会[J].渔业信息与战略,2012,27(3):194-202.DOI:10.3969/j.issn.1004-8340.2012.03.004.
[4]虞宝存,朱文斌,陈峰,等.犬牙南极鱼类渔业资源利用现状[J].河北渔业,2013(7):12-14.DOI:10.3969/j.issn.1004-6755.2013.07.005.
[5]陈宇欢,李静,范亚苇,等.脂质组学及其在营养与健康研究中的应用研究进展[J].食品科学,2014,35(15):272-276.DOI:10.7506/spkx1002-6630-201415055.
[6]SHEN Q,DAI Z Y,HUANG Y W,et al.Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry[J].Food Chemistry,2016,205:89-96.DOI:10.1016/j.foodchem.2016.02.151.
[7]LI J,VOSEGAARD T,GUO Z,et al.Applications of nuclear magnetic resonance in lipid analyses:an emerging powerful tool for lipidomics studies[J].Progress in Lipid Research,2017,68:37-56.DOI:10.1016/j.plipres.2017.09.003.
[8]QUEHENBERGER O,ARMANDO A M,DENNIS E A.High sensitivity quantitative lipidomics analysis of fatty acids in biological samples by gas chromatography-mass spectrometry[J].BBA-Molecular and Cell Biology of Lipids,2011,1811(11):648-656.DOI:10.1016/j.bbalip.2011.07.006.
[9]JUROWSKI K,KOCHAN K,WALCZAK J,et al.Analytical techniques in lipidomics:state of the art[J].Critical Reviews in Analytical Chemistry,2017,47(5):418-437.DOI:10.1080/10408347.2017.1310613.
[10]SHEN Q,MEI Y,LI L,et al.Graphene/TiO2,nanocomposite based solid-phase extraction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for lipidomic profiling of avocado(Persea americana,Mill.)[J].Analytica Chimica Acta,2014,852:153-161.DOI:10.1016/j.aca.2014.09.022.
[11]JIN R Y,LI L Q,FENG J L,et al.Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis[J].Food Chemistry,2017,216:347-354.DOI:10.1016/j.foodchem.2016.08.074.
[12]SHEN Q,CHEUNG H Y.TiO2/SiO2 core-shell composite-based sample preparation method for selective extraction of phospholipids from shrimp waste followed by hydrophilic interaction chromatography coupled with quadrupole time-of-flight/mass spectrometry analysis[J].Journal of Agricultural and Food Chemistry,2014,62(36):8944-8951.DOI:10.1021/jf503040p.
[13]STRITTMATTER N,REBEC M,JONES E A,et al.Characterization and identification of clinically relevant microorganisms using rapid evaporative ionization mass spectrometry[J].Analytical Chemistry,2014,86(13):6555-6562.DOI:10.1021/ac501075f.
[14]SCH?FER K C,DéNES J,ALBRECHT K,et al.In vivo,in situ tissue analysis using rapid evaporative ionization mass spectrometry[J].Angewandte Chemie,2010,48(44):8240-8242.DOI:10.1002/anie.200902546.
[15]STRITTMATTER N,LOVRICS A,SESSLER J,et al.Shotgun lipidomic profiling of the NCI60 cell line panel using rapid evaporative ionization mass spectrometry[J].Analytical Chemistry,2016,88(15):7507-7514.DOI:10.1021/acs.analchem.6b00187.
[16]LIN Y N,WANG H X,RAO W,et al.Rapid evaporative ionization mass spectrometry based lipidomics tracking of grass carp(Ctenopharyngodon idellus)during in vitro multiple-stage digestion[J].Journal of Agricultural and Food Chemistry,2018,66(24):6246-6253.DOI:10.1021/acs.jafc.8b01644.
[17]VERPLANKEN K,STEAD S,JANDOVA R,et al.Rapid evaporative ionization mass spectrometry for high-throughput screening in food analysis:the case of boar taint[J].Talanta,2017,169:30-36.DOI:10.1016/j.talanta.2017.03.056.
[18]BALOG J,SASI-SZABO,L,KINROSS J,et al.Intraoperative tissue identification using rapid evaporative ionization mass spectrometry[J].Science Translational Medicine,2013,194:153-154.DOI:10.1126/scitranslmed.3005623.
[19]缪圣赐,邱卫华,周雨思,等.南极周边海域犬牙鱼资源及其渔业概况[J].渔业信息与战略,2015,30(1):61-65.DOI:10.13233/j.cnki.fishis.2015.01.011.
[20]SHEN Q,WANG Y,GONG L,et al.Shotgun lipidomics strategy for fast analysis of phospholipids in fisheries waste and its potential in species differentiation[J].Journal of Agricultural and Food Chemistry,2012,60(37):9384-9393.DOI:10.1021/jf303181s.
[21]XU P P,WANG Y,CHEN J J,et al.Lipidomic profiling of juvenile yellow head catfish(Pelteobagrus fulvidraco)in response to fucoidan diet[J].Aquaculture International,2017,25(3):1123-1143.DOI:10.1007/s1049.
[22]LIN Y,WANG H,RAO W,et al.Structural characteristics of dietary fiber(Vigna radiata L.Hull)and its inhibitory effect on phospholipid digestion revealed by novel rapid evaporative ionization mass spectrometry based lipidomics[J].Food Control,2019,98:74-81.DOI:10.1016/j.foodcont.2018.11.016.
[23]BALOG J,PERENYI D,GUALLARHOYAS C,et al.Identification of the species of origin for meat products by rapid evaporative ionization mass spectrometry[J].Journal of Agricultural and Food Chemistry,2016,64(23):4793-4800.DOI:10.1021/acs.jafc.6b01041.
[24]柯庆青,李诗言,周凡,等.基于通过型固相萃取-高效液相色谱-串联质谱法同时测定水产品中的氯霉素和红霉素[J].食品科学,2017,38(24):241-246.DOI:10.7506/spkx1002-6630-201724039.
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