纳米生物活性玻璃的制备及其对人成纤维细胞增殖性能的影响
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摘要
该文采用溶胶–凝胶结合碱性共沉淀法和冷冻干燥技术制备纳米生物活性玻璃(NBG),通过SEM、TEM、激光粒度考察NBG的形貌、分散性和粒径,并通过四唑盐比色法(MTT)研究其浸提液对人成纤维细胞(HDF)增殖性能的影响。结果显示, NBG颗粒粒径小于50 nm,激光粒度仪检测到的平均粒径为491.8 nm,相比于溶胶–凝胶生物活性玻璃(SGBG), NBG平均粒径更小,分散性更好。NBG与SGBG浸提液在5倍、10倍和15倍稀释的浓度下均有利于HDF的增殖,但SGBG在早期稍有利于细胞增殖但作用不明显,而NBG则在后期对细胞增殖作用更显著,因此NBG在创面修复方面更具有应用前景。
Nanoscale bioactive glass(NBG) was prepared using the sol-gel co-precipitation and freeze-drying methods in this study. Their morphology, dispersibility and average size were investigated by using SEM, TEM and laser particle analyzer, and the effects of their extract on the proliferation of human dermal fibroblasts(HDF) were studied by methyl thiazolyl tetrazolim(MTT) assay. The results showed that the NBG particle size was less than 50 nm, while the average particle size of NBG was 491.8 nm detected by Laser particle size analyzer which was less than that of sol-gel bioactive glass(SGBG), and the dispersibility of NBG was better than that of SGBG. Furthermore, MTT assay results showed that both SGBG and NBG extracts could stimulate the proliferation of HDF in appropriate concentration at 5, 10 and 15 times dilution. However, SGBG had better stimulating effect at the early culture time, while the NBG had more obvious effect at the late stage of the culture time. Therefore, NBG is more promising in wound repair.
Nanoscale bioactive glass(NBG) was prepared using the sol-gel co-precipitation and freeze-drying methods in this study. Their morphology, dispersibility and average size were investigated by using SEM, TEM and laser particle analyzer, and the effects of their extract on the proliferation of human dermal fibroblasts(HDF) were studied by methyl thiazolyl tetrazolim(MTT) assay. The results showed that the NBG particle size was less than 50 nm, while the average particle size of NBG was 491.8 nm detected by Laser particle size analyzer which was less than that of sol-gel bioactive glass(SGBG), and the dispersibility of NBG was better than that of SGBG. Furthermore, MTT assay results showed that both SGBG and NBG extracts could stimulate the proliferation of HDF in appropriate concentration at 5, 10 and 15 times dilution. However, SGBG had better stimulating effect at the early culture time, while the NBG had more obvious effect at the late stage of the culture time. Therefore, NBG is more promising in wound repair.
引文
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2 Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res 1971; 5(6):117-41.
3 Hench LL, Paschall HA. Histochemical responses at a biomaterial’s interface. J Biomed Mater Res 1974; 8(3):49-64.
4 Gatti AM, Valdre G, Andersson OH. Analysis of the in vivo reactions of a bioactive glass in soft and hard tissue. Biomaterials1994; 15(3):208-12.
5 Verrier S, Blaker JJ, Maquet V, Hench LL, Boccaccini AR. PDLLA/Bioglass composites for soft-tissue and hard-tissue engineering:an in vitro cell biology assessment. Biomaterials 2004; 25(15):3013-21.
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7 Day RM, Boccaccini AR, Shurey S, Roether JA, Forbes A, Hench LL, et al. Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds. Biomaterials 2004;25(27):5857-66.
8 Keshaw H, Forbes A, Day RM. Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials 2005; 26(19):4171-9.
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13 吕均香,渠鹏程,翟学英.肌肤生治疗麻风溃疡疗效观察.中国麻风皮肤病杂志(LüJunxiang, Qu Pengcheng, Zhai Xueying.Observation on curative effect of skin hygiene on leprosy ulcer.Chin J Lepr Skin Dis)2005; 21(4):319.
14 胡晓燕,王光毅,程大胜,肖仕初,夏照帆.生物活性修复材料—康倍治疗烧伤的疗效观察.中国修复重建外科杂志(Hu Xiaoyan, Wang Guangyi, Cheng Dasheng, Xiao sichu, Xia zhaofan. Study on efficacy and safety of bioactive materialcombest in treating burn. Chin J Reparative Reconstructive Surg)2007; 21(11):1216-8.
15 Moosvi SR, Day RM. Bioactive glass modulation of intestinal epithelial cell restitution. Acta Biomater 2009; 5(1):76-83.
16 Day RM. Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro. Tissue Eng 2005; 11(5/6):768-77.
17 陈晓峰,李玉莉,赵娜如.溶胶-凝胶生物活性玻璃的纳米结构分析研究.硅酸盐通报(Chen xiaofeng, Li Yuli, Zhao Naru.Nano-structure analysis of the sol-gel derived bioactive glasses.B Chin ceram soc)2007; 26(2):247-51.
18 Pawlik, J, Ziabka M, Lach R, Laczka M, Katarzyna CK. Tailoring the porosity, mechanical and bioactive properties of sol-gel bioactive glasses, hydroxyapatite and titanium dioxide porous composites. J Mech Behav Biomed 2018; 87:40-9.
19 Chen XF, Lei B, Wang YJ, Zhao NR. Morphological control and in vitro, bioactivity of nanoscale bioactive glasses. J Non-Cryst Solids 2009; 355(13):791-6.
20 Liu B, You Y, Zhang H, Wu H. Synthesis of ZnO nano-powders via a novel PVA-assisted freeze-drying process. Rsc Adv 2016;6(111):110349-55.
21 Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 2011; 32(11):2757-74.
2 Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res 1971; 5(6):117-41.
3 Hench LL, Paschall HA. Histochemical responses at a biomaterial’s interface. J Biomed Mater Res 1974; 8(3):49-64.
4 Gatti AM, Valdre G, Andersson OH. Analysis of the in vivo reactions of a bioactive glass in soft and hard tissue. Biomaterials1994; 15(3):208-12.
5 Verrier S, Blaker JJ, Maquet V, Hench LL, Boccaccini AR. PDLLA/Bioglass composites for soft-tissue and hard-tissue engineering:an in vitro cell biology assessment. Biomaterials 2004; 25(15):3013-21.
6 Zhang K, Ma Y, Francis LF. Porous polymer/bioactive glass composites for soft-to-hard tissue interfaces. J Biomed Mater Res2002; 61(4):551-63.
7 Day RM, Boccaccini AR, Shurey S, Roether JA, Forbes A, Hench LL, et al. Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds. Biomaterials 2004;25(27):5857-66.
8 Keshaw H, Forbes A, Day RM. Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials 2005; 26(19):4171-9.
9 Leach KJ, Kaigler D, Wang Z, Krebsbach PH, Mooney DJ.Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. Biomaterials 2006; 27(17):3249-55.
10 Xynos ID, Edgar AJ, Buttery LD, Hench LL, Polak JM. Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochem Biophys Res Commun 2000; 276(2):461-5.
11 Eberhard J, Reimers N, Dommish H, Hacker J, Freitag S, Acil Y, Alber HK, et al. The effect of the topical administration of bioactive glass on in?ammatory markers of human experimental gingivitis. Biomaterials 2005; 26(13):1545-51.
12 罗自通.德莫林治疗糖尿病足部溃疡创面疗效观察.重庆医学(Luo Zitong. Observation on curative effect of demulin on diabetic foot ulcer. Chongqing Med J)2006; 35(9):770.
13 吕均香,渠鹏程,翟学英.肌肤生治疗麻风溃疡疗效观察.中国麻风皮肤病杂志(LüJunxiang, Qu Pengcheng, Zhai Xueying.Observation on curative effect of skin hygiene on leprosy ulcer.Chin J Lepr Skin Dis)2005; 21(4):319.
14 胡晓燕,王光毅,程大胜,肖仕初,夏照帆.生物活性修复材料—康倍治疗烧伤的疗效观察.中国修复重建外科杂志(Hu Xiaoyan, Wang Guangyi, Cheng Dasheng, Xiao sichu, Xia zhaofan. Study on efficacy and safety of bioactive materialcombest in treating burn. Chin J Reparative Reconstructive Surg)2007; 21(11):1216-8.
15 Moosvi SR, Day RM. Bioactive glass modulation of intestinal epithelial cell restitution. Acta Biomater 2009; 5(1):76-83.
16 Day RM. Bioactive glass stimulates the secretion of angiogenic growth factors and angiogenesis in vitro. Tissue Eng 2005; 11(5/6):768-77.
17 陈晓峰,李玉莉,赵娜如.溶胶-凝胶生物活性玻璃的纳米结构分析研究.硅酸盐通报(Chen xiaofeng, Li Yuli, Zhao Naru.Nano-structure analysis of the sol-gel derived bioactive glasses.B Chin ceram soc)2007; 26(2):247-51.
18 Pawlik, J, Ziabka M, Lach R, Laczka M, Katarzyna CK. Tailoring the porosity, mechanical and bioactive properties of sol-gel bioactive glasses, hydroxyapatite and titanium dioxide porous composites. J Mech Behav Biomed 2018; 87:40-9.
19 Chen XF, Lei B, Wang YJ, Zhao NR. Morphological control and in vitro, bioactivity of nanoscale bioactive glasses. J Non-Cryst Solids 2009; 355(13):791-6.
20 Liu B, You Y, Zhang H, Wu H. Synthesis of ZnO nano-powders via a novel PVA-assisted freeze-drying process. Rsc Adv 2016;6(111):110349-55.
21 Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 2011; 32(11):2757-74.
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