遮光下糠椴幼苗的适应性研究
|
摘要
以糠椴(Tilia amurensis)幼苗为材料,运用光合测定系统和叶绿素荧光技术,从光合参数角度探究糠椴幼苗对夏季遮光环境的生态适应性。结果表明,遮光后幼苗的株高、叶面积和叶片生长量均显著增加,生长性状表现良好。在轻度遮光下幼苗的生长更加旺盛,其净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和水分利用效率(WUE)均呈上升趋势;幼苗的初始荧光(Fo)降低,最大荧光(Fm)、最大光化学效率(Fv/Fm)和PSⅡ量子效率(Fv/Fo)均显著增加;能量分配比率参数φDo降低,其余均显著增加;反应中心活性参数DIO/RC降低趋势明显,其余参数均有所增加,遮光提高了幼苗PSⅡ反应中心活性。各参数在处理第21天或第28天变化最明显。结合隶属函数认为糠椴幼苗对轻度遮光环境有良好的生态适应性,适宜的遮光环境可以缓解高光高温对幼苗的胁迫作用,提高幼苗光合能力,并且处于旺盛生长期的幼苗对遮光响应更强烈。
In this study,the Tilia amurensis seedlings were used as test materials,and the photosynthesis system and chlorophyll fluorescence technique were used to explore the ecological adaptability of alfalfa seedlings to summer shading environment from the perspective of photosynthetic parameters.The results showed that the plant height,leaf area and leaf growth of the seedlings after shading increased significantly,and the growth traits performed well. The seedlings grew more vigorously under light shading. The net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr) and water use efficiency(WUE) all showed an upward trend. The initial fluorescence(Fo) of the seedlings decreased,the maximum fluorescence(Fm),maximum photochemical efficiency(Fv/Fm) and PSⅡ quantum efficiency(Fv/Fo) increased significantly,the energy distribution ratio parameter φDo decreased,and the rest increased significantly; The activity parameters of DIO/RC decreased significantly,and other parameters increased. Shading increased the activity of PSⅡ reaction center in seedlings.The parameters changed most significantly on the 21 st or 28 th day of treatment. Combined with the membership function,it is considered that the seedlings have good ecological adaptability to the light shading environment. The suitable shading environment can alleviate the stress of high light and high temperature on the seedlings,improve the photosynthetic capacity of the seedlings,and the seedlings in the vigorous growth period respond more strongly to the shading.
In this study,the Tilia amurensis seedlings were used as test materials,and the photosynthesis system and chlorophyll fluorescence technique were used to explore the ecological adaptability of alfalfa seedlings to summer shading environment from the perspective of photosynthetic parameters.The results showed that the plant height,leaf area and leaf growth of the seedlings after shading increased significantly,and the growth traits performed well. The seedlings grew more vigorously under light shading. The net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr) and water use efficiency(WUE) all showed an upward trend. The initial fluorescence(Fo) of the seedlings decreased,the maximum fluorescence(Fm),maximum photochemical efficiency(Fv/Fm) and PSⅡ quantum efficiency(Fv/Fo) increased significantly,the energy distribution ratio parameter φDo decreased,and the rest increased significantly; The activity parameters of DIO/RC decreased significantly,and other parameters increased. Shading increased the activity of PSⅡ reaction center in seedlings.The parameters changed most significantly on the 21 st or 28 th day of treatment. Combined with the membership function,it is considered that the seedlings have good ecological adaptability to the light shading environment. The suitable shading environment can alleviate the stress of high light and high temperature on the seedlings,improve the photosynthetic capacity of the seedlings,and the seedlings in the vigorous growth period respond more strongly to the shading.
引文
[1] 许大全.光合作用学[M].北京:科学出版社,2013.
[2] Foyer C H,Noctor G.Leaves in the dark see the light[J].Science,2000,284(284):5414-5416.
[3] 许大全.植物光胁迫研究中的几个问题[J].植物生理学通讯,2003,39(5):493-495.
[4] 杨虎彪,刘国道.不同光照强度对幼龄期鹧鸪茶生长的影响[J].热带作物学报,2017,38(11):2056-2059.
[5] 刘庆超,刘庆华,马宗骧,等.三桠乌药耐阴性研究[J].草业学报,2013,22(6):93-99.
[6] 李冬林,金雅琴,崔梦凡,等.遮光对连香树幼苗光合特性及其叶片解剖结构的影响[J].西北植物学报,2019,39(6):1053-1063.
[7] Scholes J D,Press M C,Zipperlen S W.Differences in light energy utilisation and dissipation between dipterocarp rain forest tree seedlings[J].Oecologia,1997,109(1):41-48.
[8] 缴丽莉,路丙社,周如久,等.遮光对青榨槭光合速率及叶绿素荧光参数的影响[J].园艺学报,2007,34(1):173-178.
[9] 李东胜,白庆红,李永杰,等.光照条件对蒙古栎幼苗生长特性和光合特征的影响[J].生态学杂志,2017,36(10):2744-2750.
[10] 马瑶,王红梅,穆立蔷,等.紫椴与糠椴枝叶解剖构造比较分析[J].齐齐哈尔大学学报,2006,22(6):93-96.
[11] 刘云强,杨建民,彭伟秀,等.两种锻树嫩枝扦插生根的解剖学研究田[J].河北农业大学学报,2004,27(2):33-37.
[12] 冯永谦,罗义成,李凤玉.锻树蜜高产的几项措施田[J].蜜蜂杂志,1999(6):5-6.
[13] 鲁敏,李伟,李小松.绿化树种对大气氯污染的反应田[J].山东建筑工程学院学报,2004,19(3):38-42.
[14] 陈明和,张景根,腾国旭.遮荫对紫椴苗木生长及光合特性影响研究[J].吉林林业科技,2008,37(5):4-7.
[15] 孙欣欣.遮荫对胡桃楸和紫椴苗木形态和生理的影响[D].哈尔滨:东北林业大学,2013.
[16] 王建华,任士福,史宝胜,等.遮光对连翘光合特性和叶绿素荧光参数的影响[J].生态学报,2011,31(7):1811-1817.
[17] 陈芳,唐秀光,董倩,等.金叶白蜡色素含量与叶色参数的年变化规律研究[J].河北农业大学学报,2012,35(5):20-24.
[18] 孟庆立,关周博,冯佰利,等.谷子抗旱相关性状的主成分与模糊聚类分析[J].中国农业科学,2009,42(8):2667-2675.
[19] 刘从,田甜,李珊,等.中国木本植物幼苗生长对光照强度的响应[J].生态学报,2018,38(2):518-527.
[20] 洪明,郭泉水,聂必红,等.三峡库区消落带狗牙根种群对水陆生境变化的响应[J].应用生态学报,2011,22(11):2829-2835.
[21] 倪建中,王伟,郁书君,等.不同种源木棉生长及光合特性研究[J].南京林业大学学报(自然科学版),2015,39(6):185-189.
[22] Cai J G,Wei M Q,Zhang Y,et al.Effects of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Hydrangea macrophylla[J].Chinese Journal of Plant Ecology,2017,41(5):570-576.
[23] Graham D Farquhar,Thomas D Sharkey.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(33):317-345.
[24] Zhang D,Zhang Q S,Yang X Q,et al.The alternation between PSⅡ and PSⅠ in ivy (Hedera nepalensis) demonstrated by in vivo chlorophyll a fluorescence and modulated 820 nm reflection[J].Plant Physiology and Biochemistry,2016,108:499-506.
[25] 时向东,汪文杰,王卫武,等.遮荫下氮肥用量对雪茄外包皮烟叶光合特性的调控效应[J].植物营养与肥料学报,2007,13(2):299-304.
[26] 魏胜利.六种药用植物的耐阴性研究[D].保定:河北农业大学,2000.
[27] 王继永,王文全,刘勇.林药间作系统中药用植物光合生理适应性规律研究[J].林业科学研究,2003,16(2):129-134.
[28] 贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
[2] Foyer C H,Noctor G.Leaves in the dark see the light[J].Science,2000,284(284):5414-5416.
[3] 许大全.植物光胁迫研究中的几个问题[J].植物生理学通讯,2003,39(5):493-495.
[4] 杨虎彪,刘国道.不同光照强度对幼龄期鹧鸪茶生长的影响[J].热带作物学报,2017,38(11):2056-2059.
[5] 刘庆超,刘庆华,马宗骧,等.三桠乌药耐阴性研究[J].草业学报,2013,22(6):93-99.
[6] 李冬林,金雅琴,崔梦凡,等.遮光对连香树幼苗光合特性及其叶片解剖结构的影响[J].西北植物学报,2019,39(6):1053-1063.
[7] Scholes J D,Press M C,Zipperlen S W.Differences in light energy utilisation and dissipation between dipterocarp rain forest tree seedlings[J].Oecologia,1997,109(1):41-48.
[8] 缴丽莉,路丙社,周如久,等.遮光对青榨槭光合速率及叶绿素荧光参数的影响[J].园艺学报,2007,34(1):173-178.
[9] 李东胜,白庆红,李永杰,等.光照条件对蒙古栎幼苗生长特性和光合特征的影响[J].生态学杂志,2017,36(10):2744-2750.
[10] 马瑶,王红梅,穆立蔷,等.紫椴与糠椴枝叶解剖构造比较分析[J].齐齐哈尔大学学报,2006,22(6):93-96.
[11] 刘云强,杨建民,彭伟秀,等.两种锻树嫩枝扦插生根的解剖学研究田[J].河北农业大学学报,2004,27(2):33-37.
[12] 冯永谦,罗义成,李凤玉.锻树蜜高产的几项措施田[J].蜜蜂杂志,1999(6):5-6.
[13] 鲁敏,李伟,李小松.绿化树种对大气氯污染的反应田[J].山东建筑工程学院学报,2004,19(3):38-42.
[14] 陈明和,张景根,腾国旭.遮荫对紫椴苗木生长及光合特性影响研究[J].吉林林业科技,2008,37(5):4-7.
[15] 孙欣欣.遮荫对胡桃楸和紫椴苗木形态和生理的影响[D].哈尔滨:东北林业大学,2013.
[16] 王建华,任士福,史宝胜,等.遮光对连翘光合特性和叶绿素荧光参数的影响[J].生态学报,2011,31(7):1811-1817.
[17] 陈芳,唐秀光,董倩,等.金叶白蜡色素含量与叶色参数的年变化规律研究[J].河北农业大学学报,2012,35(5):20-24.
[18] 孟庆立,关周博,冯佰利,等.谷子抗旱相关性状的主成分与模糊聚类分析[J].中国农业科学,2009,42(8):2667-2675.
[19] 刘从,田甜,李珊,等.中国木本植物幼苗生长对光照强度的响应[J].生态学报,2018,38(2):518-527.
[20] 洪明,郭泉水,聂必红,等.三峡库区消落带狗牙根种群对水陆生境变化的响应[J].应用生态学报,2011,22(11):2829-2835.
[21] 倪建中,王伟,郁书君,等.不同种源木棉生长及光合特性研究[J].南京林业大学学报(自然科学版),2015,39(6):185-189.
[22] Cai J G,Wei M Q,Zhang Y,et al.Effects of shading on photosynthetic characteristics and chlorophyll fluorescence parameters in leaves of Hydrangea macrophylla[J].Chinese Journal of Plant Ecology,2017,41(5):570-576.
[23] Graham D Farquhar,Thomas D Sharkey.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(33):317-345.
[24] Zhang D,Zhang Q S,Yang X Q,et al.The alternation between PSⅡ and PSⅠ in ivy (Hedera nepalensis) demonstrated by in vivo chlorophyll a fluorescence and modulated 820 nm reflection[J].Plant Physiology and Biochemistry,2016,108:499-506.
[25] 时向东,汪文杰,王卫武,等.遮荫下氮肥用量对雪茄外包皮烟叶光合特性的调控效应[J].植物营养与肥料学报,2007,13(2):299-304.
[26] 魏胜利.六种药用植物的耐阴性研究[D].保定:河北农业大学,2000.
[27] 王继永,王文全,刘勇.林药间作系统中药用植物光合生理适应性规律研究[J].林业科学研究,2003,16(2):129-134.
[28] 贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |