不同生物膜载体下MBBR中微生物群落变化特征
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摘要
以沸石粉和聚氨酯海绵为原料制备新型生物膜载体,对比分析了投加新型载体和传统聚氨酯海绵载体的移动床生物膜反应器(MBBR)的微生物群落结构及功能性脱氮菌属的演替变化特征。结果表明,微生物在新型载体和传统聚氨酯海绵载体上的生长速率分别为0. 009 5和0. 006 5 g/(g载体·d)。通过对微生物群落的分析,两种生物膜载体上的菌群存在较大的差异性,但Proteobacteria和Bacteroidetes在整个试验过程中是最占优势的两种菌。Shannon、ACE和Chao1等指数表明新型载体上微生物多样性更加丰富稳定,并且存在较多的反硝化功能性菌属(Sphaerotilus、Flavobacterium和Thermomonas等),从而提高了MBBR工艺的脱氮性能。
New biofilm carrier was prepared using zeolite powders and polyurethane sponges as raw materials. The succession changes in microbial community and functional denitrification bacteria in the moving bed biofilm reactor( MBBR) with new biofilm carriers and traditional polyurethane sponge carriers were compared and analyzed. The results showed that the growth rates of microorganisms on the new biofilm carriers and the traditional polyurethane sponge carriers were 0. 009 5 g/( g·d) and 0. 006 5 g/( g·d),respectively. The analysis of microbial community showed great differences between the two types of biofilm carriers; however,Proteobacteria and Bacteroidetes were the two dominant bacteria in the whole experiment process. Shannon,ACE,and Chao1 indices showed that the microbial diversity on the new biofilm carrier was more abundant and stable,and there were more denitrification functional bacteria( Sphaerotilus,Flavobacterium,Thermomonas,etc.) on the new biofilm carrier,which improved the nitrogen removal performance in the MBBR process.
New biofilm carrier was prepared using zeolite powders and polyurethane sponges as raw materials. The succession changes in microbial community and functional denitrification bacteria in the moving bed biofilm reactor( MBBR) with new biofilm carriers and traditional polyurethane sponge carriers were compared and analyzed. The results showed that the growth rates of microorganisms on the new biofilm carriers and the traditional polyurethane sponge carriers were 0. 009 5 g/( g·d) and 0. 006 5 g/( g·d),respectively. The analysis of microbial community showed great differences between the two types of biofilm carriers; however,Proteobacteria and Bacteroidetes were the two dominant bacteria in the whole experiment process. Shannon,ACE,and Chao1 indices showed that the microbial diversity on the new biofilm carrier was more abundant and stable,and there were more denitrification functional bacteria( Sphaerotilus,Flavobacterium,Thermomonas,etc.) on the new biofilm carrier,which improved the nitrogen removal performance in the MBBR process.
引文
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[10] Luo Y L,Guo W S,Ngo H H,et al. Removal and fate of micropollutants in a sponge-based moving bed bioreactor[J]. Bioresour Technol,2014,159:311-319.
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[13] Wang X D,Bi X J,Hem L J,et al. Microbial community composition of a multi-stage moving bed biofilm reactor and its interaction with kinetic model parameters estimation[J]. J Environ Manage,2018,218:340-347.
[14] Atabek A,Camesano T A. Atomic force microscopy study of the effect of lipopolysaccharides and extracellular polymers on adhesion of Pseudomonas aeruginosa[J]. J Bacteriol,2007,189(23):8503-8509.
[15] Tang B,Chen Q Y,Bin L Y,et al. Insight into the microbial community and its succession of a coupling anaerobicaerobic biofilm on semi-suspended bio-carriers[J].Bioresour Technol,2017,247:591-598.
[16] Huang C,Li Z L,Chen F,et al. Microbial community structure and function in response to the shift of sulfide/nitrate loading ratio during the denitrifying sulfide removal process[J]. Bioresour Technol,2015,197:227-234.
[2]张鹏,袁辉洲,柯水洲. MBBR法处理城市污水去除污染物的特性研究[J].水处理技术,2009,35(10):91-96.Zhang Peng,Yuan Huizhou,Ke Shuizhou. Study on the characters of treating urban sewage to remove pollutants with MBBR process[J]. Technology of Water Treatment,2009,35(10):91-96(in Chinese).
[3]王学江,夏四清,陈玲,等. DO对MBBR同步硝化反硝化生物脱氮影响研究[J].同济大学学报:自然科学版,2006,34(4):514-517,538.Wang Xuejiang,Xia Siqing,Chen Ling,et al. Effect of DO on smiultaneous nitrification and denitrification in MBBR[J]. Journal of Tongji University:Natural Science,2006,34(4):514-517,538(in Chinese).
[4]张新波,宋姿,祁丽,等.聚氨酯海绵载体填充率对MBBR脱氮的影响[J].中国给水排水,2017,33(15):78-81.Zhang Xinbo,Song Zi,Qi Li,et al. Effect of filling fraction on nitrogen removal of polyurethane spongebased moving bed biofilm reactor[J]. China Water&Wastewater,2017,33(15):78-81(in Chinese).
[5]孟涛,刘杰,杨超,等. MBBR工艺用于青岛李村河污水处理厂升级改造[J].中国给水排水,2013,29(2):59-61.Meng Tao,Liu Jie,Yang Chao,et al. Application of MBBR process in upgrading and reconstruction of Licunhe WWTP in Qingdao City[J]. China Water&Wastewater,2013,29(2):59-61(in Chinese).
[6]王翥田,叶亮,张新彦,等. MBBR工艺用于无锡芦村污水处理厂的升级改造[J].中国给水排水,2010,26(2):71-73.Wang Zhutian, Ye Liang, Zhang Xinyan, et al.Application of MBBR process to upgrading and reconstruction of WWTP[J]. China Water&Wastewater,2010,26(2):71-73(in Chinese).
[7] Zhang X B,Song Z,Guo W S,et al. Behavior of nitrogen removal in an aerobic sponge based moving bed biofilm reactor[J]. Bioresour Technol,2017,245(Pt A):1282-1285.
[8]方茜,张朝升,张立秋,等.曝气量对同时硝化/反硝化除磷工艺效能的影响[J].中国给水排水,2014,30(21):14-18.Fang Qian,Zhang Chaosheng,Zhang Liqiu,et al.Influence of aeration rate on simultaneous nitrification/denitrification phosphorus removal[J]. China Water&Wastewater,2014,30(21):14-18(in Chinese).
[9] Zhang X B,Chen X,Zhang C Q,et al. Effect of filling fraction on the performance of sponge-based moving bed biofilm reactor[J]. Bioresour Technol,2016,219:762-767.
[10] Luo Y L,Guo W S,Ngo H H,et al. Removal and fate of micropollutants in a sponge-based moving bed bioreactor[J]. Bioresour Technol,2014,159:311-319.
[11] Guo W S,Ngo H H,Dharmawan F,et al. Roles of polyurethane foam in aerobic moving and fixed bed bioreactors[J]. Bioresour Technol,2010,101(5):1435-1439.
[12] Wang L F,Li Y,Wang L,et al. Responses of biofilm microorganisms from moving bed biofilm reactor to antibiotics exposure:Protective role of extracellular polymeric substances[J]. Bioresour Technol,2018,254:268-277.
[13] Wang X D,Bi X J,Hem L J,et al. Microbial community composition of a multi-stage moving bed biofilm reactor and its interaction with kinetic model parameters estimation[J]. J Environ Manage,2018,218:340-347.
[14] Atabek A,Camesano T A. Atomic force microscopy study of the effect of lipopolysaccharides and extracellular polymers on adhesion of Pseudomonas aeruginosa[J]. J Bacteriol,2007,189(23):8503-8509.
[15] Tang B,Chen Q Y,Bin L Y,et al. Insight into the microbial community and its succession of a coupling anaerobicaerobic biofilm on semi-suspended bio-carriers[J].Bioresour Technol,2017,247:591-598.
[16] Huang C,Li Z L,Chen F,et al. Microbial community structure and function in response to the shift of sulfide/nitrate loading ratio during the denitrifying sulfide removal process[J]. Bioresour Technol,2015,197:227-234.
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