外源H_2O_2对盐碱混合胁迫下裸燕麦幼苗生长和抗性生理的影响
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摘要
为探讨信号分子过氧化氢(H_2O_2)增强裸燕麦盐碱耐性的作用及其生理机制,以裸燕麦品种‘定莜6号’为材料,在日光温室内用珍珠岩培养幼苗至三叶一心期时叶面喷施0. 01 mmol·L~(-1)H_2O_2的同时根部浇灌75mmol·L~(-1)盐碱混合溶液(NaCl∶Na_2SO_4∶NaHCO_3∶Na_2CO_3=12∶8∶9∶1)或添加H_2O_2淬灭剂二甲基硫脲(DMTU),研究对幼苗生长及叶片光合色素含量、活性氧代谢和渗透调节物质积累的影响。结果表明:喷施H_2O_2能够缓解盐碱混合胁迫对裸燕麦幼苗生长的抑制,提高幼苗根长、株高和植株干重及叶片叶绿素a、叶绿素b、总叶绿素、类胡萝卜素含量和超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶活性,降低超氧阴离子、H_2O_2、丙二醛、抗坏血酸、谷胱甘肽和游离氨基酸含量,促进抗氧化物质类黄酮、总酚和原花青素及渗透调节物质可溶性蛋白质、可溶性糖和脯氨酸积累。添加DMTU部分或完全逆转了H_2O_2的上述作用。采用隶属函数综合评价显示,喷施H_2O_2提高了盐碱混合胁迫下裸燕麦幼苗的综合评价值D,添加DMTU完全逆转了H_2O_2对D值的提升作用。表明外源H_2O_2通过参与活性氧代谢和渗透调节物质积累等生理代谢调控缓解盐碱混合胁迫诱导的氧化伤害和生长抑制,从而提高裸燕麦对盐碱胁迫的适应能力。
The experiment was conducted to study whether exogenous hydrogen peroxide(H_2O_2) could improve the saline-alkali tolerance of naked oat(Avena nuda) seedlings and its physiological mechanism. Seedlings of naked oat(‘Dingyou No.6') at three-leaf period cultured with perlite in a solar greenhouse were irrigated with the Hoagland nutrient solution containing 75 mmol · L~(-1) saline-alkali mixed solution(NaCl ∶ Na_2SO_4∶ NaHCO_3∶ Na_2CO_3=12∶8∶9∶1) combination with dimethylthiourea(DMTU,a quencher of H_2O_2),and the leaves were sprayed with0. 01 mmol·L~(-1) H_2O_2. Seedlings were irrigated with Hoagland nutrient solution,and the leaves were sprayed with water served as the control. Seedlings growth,photosynthetic pigment content,active oxygen metabolism,and osmotic regulatory substance accumulation in leaves were measured. The results showed that exogenous H_2O_2 could alleviate the inhibition effects of saline-alkali mixed stress on seedlings growth of naked oat.Spraying H_2O_2 significantly increased root length,plant height,dry weight,the leaf chlorophyll a,chlorophyll b,total chlorophyll and carotenoid contents,and the superoxide dismutase,peroxidase,catalase,ascorbate peroxidase activities of naked oat seedlings under saline-alkali mixed stress,but decreased the contents of superoxide anion,H_2O_2,malondialdehyde,ascorbic acid,glutathione,and free amino acids. Treatments with spraying H_2O_2 also increased the contents of antioxidants(such as flavonoids,total phenols,procyanidins) and osmotic regulatory substance(such as soluble protein,soluble sugars,and proline) in leaves. The above effects of exogenous H_2O_2 were partially or completely reversed by DMTU. A comprehensive evaluation based on subordinate function analyses revealed that foliar spraying with H_2O_2 could improve the saline-alkali resistance of naked oat seedlings,and the promoting effect of H_2O_2 could be completely reversed by DMTU. Therefore,the exogenous H_2O_2 supply can increase saline-alkali adaptability in naked oat seedlings by regulating active oxygen metabolism and osmotic regulatory substance accumulation in leaves of naked oat plants under saline-alkali mixed stress.
The experiment was conducted to study whether exogenous hydrogen peroxide(H_2O_2) could improve the saline-alkali tolerance of naked oat(Avena nuda) seedlings and its physiological mechanism. Seedlings of naked oat(‘Dingyou No.6') at three-leaf period cultured with perlite in a solar greenhouse were irrigated with the Hoagland nutrient solution containing 75 mmol · L~(-1) saline-alkali mixed solution(NaCl ∶ Na_2SO_4∶ NaHCO_3∶ Na_2CO_3=12∶8∶9∶1) combination with dimethylthiourea(DMTU,a quencher of H_2O_2),and the leaves were sprayed with0. 01 mmol·L~(-1) H_2O_2. Seedlings were irrigated with Hoagland nutrient solution,and the leaves were sprayed with water served as the control. Seedlings growth,photosynthetic pigment content,active oxygen metabolism,and osmotic regulatory substance accumulation in leaves were measured. The results showed that exogenous H_2O_2 could alleviate the inhibition effects of saline-alkali mixed stress on seedlings growth of naked oat.Spraying H_2O_2 significantly increased root length,plant height,dry weight,the leaf chlorophyll a,chlorophyll b,total chlorophyll and carotenoid contents,and the superoxide dismutase,peroxidase,catalase,ascorbate peroxidase activities of naked oat seedlings under saline-alkali mixed stress,but decreased the contents of superoxide anion,H_2O_2,malondialdehyde,ascorbic acid,glutathione,and free amino acids. Treatments with spraying H_2O_2 also increased the contents of antioxidants(such as flavonoids,total phenols,procyanidins) and osmotic regulatory substance(such as soluble protein,soluble sugars,and proline) in leaves. The above effects of exogenous H_2O_2 were partially or completely reversed by DMTU. A comprehensive evaluation based on subordinate function analyses revealed that foliar spraying with H_2O_2 could improve the saline-alkali resistance of naked oat seedlings,and the promoting effect of H_2O_2 could be completely reversed by DMTU. Therefore,the exogenous H_2O_2 supply can increase saline-alkali adaptability in naked oat seedlings by regulating active oxygen metabolism and osmotic regulatory substance accumulation in leaves of naked oat plants under saline-alkali mixed stress.
引文
1.潘雄波,向丽霞,胡晓辉,等.外源亚精胺对盐碱胁迫下番茄幼苗根系线粒体功能的影响[J].应用生态学报,2016,27(2):491-498.Pan X B,Xiang L X,Hu X H,et al.Effects of exogenous spermidine on mitochondrial function of tomato seedling roots under salinity-alkalinity stress[J].Chinese Journal of Applied Ecology,2016,27(2):491-498.
2.刘建新,王金成,王瑞娟,等.混合盐碱胁迫对燕麦幼苗矿质离子吸收和光合特性的影响[J].干旱地区农业研究,2017,35(1):178-184.Liu J X,Wang J C,Wang R J,et al.Effect of complex saline-alkali stress on the mineral ions absorption and photosynthetic characteristics of oat seedlings[J].Agricultural Research in the Arid Areas,2017,35(1):178-184.
3.闫永庆,刘兴亮,王崑,等.白刺对不同浓度混合盐碱胁迫的生理响应[J].植物生态学报,2010,34(10):1213-1219.Yan Y Q,Liu X L,Wang K,et al.Effect of complex salinealkali stress on physiological parameters of Nitratia tangutorum[J].Chinese Journal of Plant Ecology,2010,34(10):1213-1219.
4.Tanou G,Molassiotis A,Diamantidis G.Hydrogen peroxideand nitric oxide-induced systemic antioxidant prime-like activity under Na Cl-stress and stress-free conditions in citrus plants[J].Journal of Plant Physiology,2009,166(17):1904-1913.
5.郭瑞,周际,杨帆,等.碱胁迫对小麦(Triticum aestivum Linn.)叶片代谢过程的影响[J].中国农业科学,2017,50(2):250-259.Guo R,Zhou J,Yang F,et al.Effects of alkaline stress on metabonomic responses of wheat(Triticum aestivum Linn.)leaves[J].Scientia Agricultura Sinica,2017,50(2):250-259.
6.李辛,赵文智.荒漠区植物雾冰藜光合特性对混合盐碱胁迫的响应[J].生态学报,2018,38(4):1183-1193.Li X,Zhao W Z.Response of various salt-alkaline mixed stresses on the photosynthetic characteristics of Bassia dasyphylla in a desert region[J].Acta Ecologica Sinica,2018,38(4):1183-1193.
7.闫永庆,王文杰,朱虹,等.混合盐碱胁迫对青山杨渗透调节物质及活性氧代谢的影响[J].应用生态学报,2009,20(9):2085-2091.Yan Y Q,Wang W J,Zhu H,et al.Effects of salt-alkali stress on osmoregulation substance and active oxygen metabolism of Qingshan poplar(Populus pseudo-cathayana×P.deltoides)[J].Chinese Journal of Applied Ecology,2009,20(9):2085-2091.
8.Jiang J L,Su M,Wang L Y,et al.Exogenous hydrogen peroxide reversibly inhibits root gravitropism and induces horizontal curvature of primary root during grass pea germination[J].Plant Physiology and Biochemistry,2012,53:84-93.
9.庞学群,潘少丽,王海波,等.过氧化氢在香蕉果实采后耐冷性诱导中的作用[J].园艺学报,2007,34(6):1373-1378.Pang X Q,Pan S L,Wang H B,et al.Role of hydrogen peroxide on chilling resistance of postharvest banana fruits[J].Acta Horticulturae Sinica,2007,34(6):1373-1378.
10.李希东,侯丽霞,刘新,等.H2O2与葡萄Vv IPK2基因表达及其低温胁迫响应的关系[J].园艺学报,2011,38(6):1052-1062.Li X D,Hou L X,Liu X,et al.Involvement of H2O2in regulating the expression of Vv IPK2 in response to low temperature stress in leaves of Vitis[J].Acta Horticulturae Sinica,2011,38(6):1052-1062.
11.Wang Y,Li J L,Wang J Z,et al.Exogenous H2O2improves the chilling tolerance of manilagrass and mascarenegrass by activating the antioxidative system[J].Plant Growth Regulation,2010,61(2):195-204.
12.安国勇,李保珠,武桂丽,等.H2O2作为根源信号介导盐胁迫诱导的蚕豆气孔关闭反应[J].植物生理学报,2012,48(3):265-271.An G Y,Li B Z,Wu G L,et al.H2O2could act as root source signal to mediate stomatal closure induced by salt stress of Vicia faba L.[J].Plant Physiology Journal,2012,48(3):265-271.
13.De Azevedo Neto A D,Prisco J T,Enéas-Filho J,et al.Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162(10):1114-1122.
14.Wahid A,Perveen M,Gelani S,et al.Pretreatment of seed with H2O2improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins[J].Journal of Plant Physiology,2007,164(3):283-294.
15.刘忠静,郭延奎,林少航,等.外源过氧化氢对干旱胁迫下温室黄瓜叶绿体超微结构和抗氧化酶的影响[J].园艺学报,2009,36(8):1140-1146.Liu Z J,Guo Y K,Lin S H,et al.Effects of exogenous hydrogen peroxide on ultrastructure of chloroplasts and activities of antioxidant enzymes in greenhouse-ecotype cucumber under drought stress[J].Acta Horticulturae Sinica,2009,36(8):1140-1146.
16.Uchida A,Jagendorf A T,Hibino T,et al.Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J].Plant Science,2002,163(3):515-523.
17.白晓娟,刘丽娟,张春华,等.H2O2预处理对不同水稻品种Cd耐性的影响[J].中国水稻科学,2010,24(4):391-397.Bai X J,Liu L J,Zhang C H,et al.Effect of H2O2pretreatment on Cd tolerance of different rice cultivars[J].Chinese Journal of Rice Science,2010,24(4):391-397.
18.刘建新,王金成,刘秀丽,等.外源H2O2对混合盐碱胁迫下燕麦幼苗叶片脯氨酸积累和代谢途径的影响[J].植物研究,2016,36(3):427-433.Liu J X,Wang J C,Liu X L,et al.Effect of exogenous H2O2on proline accumulation and metabolic pathway in leaves of oat seedlings under complex saline-alkali stress[J].Bulletin of Botanical Research,2016,36(3):427-433.
19.刘建新,欧晓彬,王金成,等.过氧化氢提高燕麦幼苗耐碱性的活性氧代谢和渗透调节[J].草业学报,2018,27(2):97-104.Liu J X,Ou X B,Wang J C,et al.Exogenous H2O2improves alkali resistance of oat seedlings by regulating active oxygen metabolism and osmolyte accumulation[J].Acta Prataculturae Sinica,2018,27(2):97-104.
20.Drzikova B,Dongowski G,Gebhardt E.Dietary fibre-rich oat-based products affect serum lipids,microbiota,formation of short-chain fatty acids and steroids in rats[J].British Journal of Nutrition,2005,94(6):1012-1025.
21.林叶春,曾昭海,任长忠,等.局部根区灌溉对裸燕麦光合特征曲线及叶绿素荧光特性的影响[J].作物学报,2012,38(6):1062-1070.Lin Y C,Zeng Z H,Ren C Z,et al.Effects of partial root zone irrigation on leaf photosynthetic curves and chlorophyll fluorescence parameters in naked oat[J].Acta Agronomica Sinica,2012,38(6):1062-1070.
22.贺学勤,张聪超.DMTU对瓶插月季花瓣中抗氧化酶和蔗糖代谢酶活性的影响[J].作物杂志,2013,(2):91-95.He X Q,Zhang C C.Effects of DMTU on activities of antioxidant enzymes and sucrose metabolizing enzymes of Rosa hydrida L.in Vase[J].Crops,2013,(2):91-95.
23.高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:16,74-77,199-222.Gao J F.Experimental guidance for plant physiology[M].Beijing:Higher Education Press,2006:16,74-77,199-222.
24.Sergiev I,Alexieva V,Karanov E.Effect of spermine,atrazine and combination between them on some endogenous protective systems and stress markers in plants[J].Comptes Rendus de I’Academie Bulgare des Sciences,1997,51:121-124.
25.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:184-197,258-261.Li H S.Principles and techniques of plant physiological biochemical Experiment[M].Beijing:Higher Education Press,2000:184-197,258-261.
26.陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:68-77.Chen J X,Wang X F.Plant physiology experiment guidance[M].Guangzhou:South China University of Technology Press,2006:68-77.
27.张志斌,颜日明,邱晓芳,等.杜仲愈伤组织与悬浮细胞中黄酮和绿原酸积累研究[J].中国中药杂志,2009,34(13):1636-1639.Zhang Z B,Yan R M,Qiu X F,et al.Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides[J].China Journal of Chinese Meteria Medica,2009,34(13):1636-1639.
28.Singleton V L,Orthofer R,Lamuela-Raventós R M.Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent[J].Methods in Enzymology,1999,299:152-178.
29.Vivas N,Nonier M F,De Gaulejac N V,et al.Differentiation of proanthocyanidin tannins from seeds,skins and stems of grapes(Vitis vinifera)and heartwood of Quebracho(Schinopsis balansae)by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and thioacidolysis/liquid chromatography/electrospray ionization mass spectrometry[J].Analytica Chimica Acta,2004,513(1):247-256.
30.Fedina I S,Nedeva D,i9ek N.Pre-treatment with H2O2induces salt tolerance in Barley seedlings[J].Biologia Plantarum,2009,53(2):321-324.
31.刘建新,王金成,刘秀丽,等.碱胁迫下燕麦幼苗对外源过氧化氢的生理响应[J].麦类作物学报,2017,37(10):1383-1389.Liu J X,Wang J C,Liu X L,et al.Effect of exogenous hydrogen peroxide on physiological responses of Avena nuda L.seedlings under alkali stress[J].Journal of Triticeae Crops,2017,37(10):1383-1389.
32.刘建新,王金成,王瑞娟,等.燕麦幼苗对盐胁迫的响应及过氧化氢对响应的调节[J].生态学杂志,2015,34(9):2506-2511.Liu J X,Wang J C,Wang R J,et al.Response of Avena nuda L.seedlings to salt stress and the modulation of hydrogen peroxide[J].Chinese Journal of Ecology,2015,34(9):2506-2511.
33.Gong B,Wen D,Vandenlangenberg K,et al.Comparative effects of Na Cl and Na HCO3stress on photosynthetic parameters,nutrient metabolism,and the antioxidant system in tomato leaves[J].Scientia Horticulturae,2013,157:1-12.
34.Nakabayashi R,Mori T,Saito K.Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana[J].Plant Signaling&Behavior,2014,9(8):e29518.
35.陈颖,杨华,乐利,等.过氧化氢对银杏悬浮细胞黄酮含量及抗氧化酶活性的影响[J].中南林业科技大学学报,2014,34(12):40-45,55.Chen Y,Yang H,Yue L,et al.Effects of exogenous hydrogen peroxide on flavonoid content and antioxidant enzyme activities in suspension cells of Ginkgo biloba L.[J].Journal of Central South University of Forestry&Technology,2014,34(12):40-45,55.
36.马廷蕊,张金文,梁慧光,等.外源NO和H2O2诱导不同颜色马铃薯花青素积累[J].中国蔬菜,2012,1(16):63-67.Ma T R,Zhang J W,Liang H G,et al.Exogenous NO and H2O2induced anthocyanin accumulation of different colors potatos[J].China Vegetables,2012,1(16):63-67.
37.张波,张怀刚.外源H2O2对小麦幼苗耐盐性的调节作用[J].西北植物学报,2007,27(12):2491-2495.Zhang B,Zhang H G.Regulation of exogenous hydrogen peroxide on wheat seedling salinity tolerance[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(12):2491-2495.
38.田武英,焦青松,石岱龙,等.胞外ATP通过H2O2对菜豆叶片光系统Ⅱ光化学特性的系统性影响[J].植物研究,2016,36(3):368-373.Tian W Y,Jiao Q S,Shi D L,et al.Extracellular ATP exert systemic effects on photosystemⅡphotochemistry of kidney bean(Phaseolus vulgaris)leaf through a H2O2signalling pathway[J].Bulletin of Botanical Research,2016,36(3):368-373.
39.柳永强,杨谋,马廷蕊,等.外源NO和H2O2诱导马铃薯对盐碱土壤的适应[J].土壤通报,2012,43(6):1380-1384.Liu Y Q,Yang M,Ma T R,et al.Exogenous NO and H2O2induced potato adaptation to saline-alkali soil[J].Chinese Journal of Soil Science,2012,43(6):1380-1384.
40.惠竹梅,王智真,胡勇,等.24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响[J].中国农业科学,2013,46(5):1005-1013.Hui Z M,Wang Z Z,Hu Y,et al.Effects of 24-epibrassinolide on the antioxidant system and osmotic adjustment substance in grape seedlings(V.vinifera L.)under chilling stress[J].Scientia Agricultura Sinica,2013,46(5):1005-1013.
2.刘建新,王金成,王瑞娟,等.混合盐碱胁迫对燕麦幼苗矿质离子吸收和光合特性的影响[J].干旱地区农业研究,2017,35(1):178-184.Liu J X,Wang J C,Wang R J,et al.Effect of complex saline-alkali stress on the mineral ions absorption and photosynthetic characteristics of oat seedlings[J].Agricultural Research in the Arid Areas,2017,35(1):178-184.
3.闫永庆,刘兴亮,王崑,等.白刺对不同浓度混合盐碱胁迫的生理响应[J].植物生态学报,2010,34(10):1213-1219.Yan Y Q,Liu X L,Wang K,et al.Effect of complex salinealkali stress on physiological parameters of Nitratia tangutorum[J].Chinese Journal of Plant Ecology,2010,34(10):1213-1219.
4.Tanou G,Molassiotis A,Diamantidis G.Hydrogen peroxideand nitric oxide-induced systemic antioxidant prime-like activity under Na Cl-stress and stress-free conditions in citrus plants[J].Journal of Plant Physiology,2009,166(17):1904-1913.
5.郭瑞,周际,杨帆,等.碱胁迫对小麦(Triticum aestivum Linn.)叶片代谢过程的影响[J].中国农业科学,2017,50(2):250-259.Guo R,Zhou J,Yang F,et al.Effects of alkaline stress on metabonomic responses of wheat(Triticum aestivum Linn.)leaves[J].Scientia Agricultura Sinica,2017,50(2):250-259.
6.李辛,赵文智.荒漠区植物雾冰藜光合特性对混合盐碱胁迫的响应[J].生态学报,2018,38(4):1183-1193.Li X,Zhao W Z.Response of various salt-alkaline mixed stresses on the photosynthetic characteristics of Bassia dasyphylla in a desert region[J].Acta Ecologica Sinica,2018,38(4):1183-1193.
7.闫永庆,王文杰,朱虹,等.混合盐碱胁迫对青山杨渗透调节物质及活性氧代谢的影响[J].应用生态学报,2009,20(9):2085-2091.Yan Y Q,Wang W J,Zhu H,et al.Effects of salt-alkali stress on osmoregulation substance and active oxygen metabolism of Qingshan poplar(Populus pseudo-cathayana×P.deltoides)[J].Chinese Journal of Applied Ecology,2009,20(9):2085-2091.
8.Jiang J L,Su M,Wang L Y,et al.Exogenous hydrogen peroxide reversibly inhibits root gravitropism and induces horizontal curvature of primary root during grass pea germination[J].Plant Physiology and Biochemistry,2012,53:84-93.
9.庞学群,潘少丽,王海波,等.过氧化氢在香蕉果实采后耐冷性诱导中的作用[J].园艺学报,2007,34(6):1373-1378.Pang X Q,Pan S L,Wang H B,et al.Role of hydrogen peroxide on chilling resistance of postharvest banana fruits[J].Acta Horticulturae Sinica,2007,34(6):1373-1378.
10.李希东,侯丽霞,刘新,等.H2O2与葡萄Vv IPK2基因表达及其低温胁迫响应的关系[J].园艺学报,2011,38(6):1052-1062.Li X D,Hou L X,Liu X,et al.Involvement of H2O2in regulating the expression of Vv IPK2 in response to low temperature stress in leaves of Vitis[J].Acta Horticulturae Sinica,2011,38(6):1052-1062.
11.Wang Y,Li J L,Wang J Z,et al.Exogenous H2O2improves the chilling tolerance of manilagrass and mascarenegrass by activating the antioxidative system[J].Plant Growth Regulation,2010,61(2):195-204.
12.安国勇,李保珠,武桂丽,等.H2O2作为根源信号介导盐胁迫诱导的蚕豆气孔关闭反应[J].植物生理学报,2012,48(3):265-271.An G Y,Li B Z,Wu G L,et al.H2O2could act as root source signal to mediate stomatal closure induced by salt stress of Vicia faba L.[J].Plant Physiology Journal,2012,48(3):265-271.
13.De Azevedo Neto A D,Prisco J T,Enéas-Filho J,et al.Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162(10):1114-1122.
14.Wahid A,Perveen M,Gelani S,et al.Pretreatment of seed with H2O2improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins[J].Journal of Plant Physiology,2007,164(3):283-294.
15.刘忠静,郭延奎,林少航,等.外源过氧化氢对干旱胁迫下温室黄瓜叶绿体超微结构和抗氧化酶的影响[J].园艺学报,2009,36(8):1140-1146.Liu Z J,Guo Y K,Lin S H,et al.Effects of exogenous hydrogen peroxide on ultrastructure of chloroplasts and activities of antioxidant enzymes in greenhouse-ecotype cucumber under drought stress[J].Acta Horticulturae Sinica,2009,36(8):1140-1146.
16.Uchida A,Jagendorf A T,Hibino T,et al.Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J].Plant Science,2002,163(3):515-523.
17.白晓娟,刘丽娟,张春华,等.H2O2预处理对不同水稻品种Cd耐性的影响[J].中国水稻科学,2010,24(4):391-397.Bai X J,Liu L J,Zhang C H,et al.Effect of H2O2pretreatment on Cd tolerance of different rice cultivars[J].Chinese Journal of Rice Science,2010,24(4):391-397.
18.刘建新,王金成,刘秀丽,等.外源H2O2对混合盐碱胁迫下燕麦幼苗叶片脯氨酸积累和代谢途径的影响[J].植物研究,2016,36(3):427-433.Liu J X,Wang J C,Liu X L,et al.Effect of exogenous H2O2on proline accumulation and metabolic pathway in leaves of oat seedlings under complex saline-alkali stress[J].Bulletin of Botanical Research,2016,36(3):427-433.
19.刘建新,欧晓彬,王金成,等.过氧化氢提高燕麦幼苗耐碱性的活性氧代谢和渗透调节[J].草业学报,2018,27(2):97-104.Liu J X,Ou X B,Wang J C,et al.Exogenous H2O2improves alkali resistance of oat seedlings by regulating active oxygen metabolism and osmolyte accumulation[J].Acta Prataculturae Sinica,2018,27(2):97-104.
20.Drzikova B,Dongowski G,Gebhardt E.Dietary fibre-rich oat-based products affect serum lipids,microbiota,formation of short-chain fatty acids and steroids in rats[J].British Journal of Nutrition,2005,94(6):1012-1025.
21.林叶春,曾昭海,任长忠,等.局部根区灌溉对裸燕麦光合特征曲线及叶绿素荧光特性的影响[J].作物学报,2012,38(6):1062-1070.Lin Y C,Zeng Z H,Ren C Z,et al.Effects of partial root zone irrigation on leaf photosynthetic curves and chlorophyll fluorescence parameters in naked oat[J].Acta Agronomica Sinica,2012,38(6):1062-1070.
22.贺学勤,张聪超.DMTU对瓶插月季花瓣中抗氧化酶和蔗糖代谢酶活性的影响[J].作物杂志,2013,(2):91-95.He X Q,Zhang C C.Effects of DMTU on activities of antioxidant enzymes and sucrose metabolizing enzymes of Rosa hydrida L.in Vase[J].Crops,2013,(2):91-95.
23.高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:16,74-77,199-222.Gao J F.Experimental guidance for plant physiology[M].Beijing:Higher Education Press,2006:16,74-77,199-222.
24.Sergiev I,Alexieva V,Karanov E.Effect of spermine,atrazine and combination between them on some endogenous protective systems and stress markers in plants[J].Comptes Rendus de I’Academie Bulgare des Sciences,1997,51:121-124.
25.李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:184-197,258-261.Li H S.Principles and techniques of plant physiological biochemical Experiment[M].Beijing:Higher Education Press,2000:184-197,258-261.
26.陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2002:68-77.Chen J X,Wang X F.Plant physiology experiment guidance[M].Guangzhou:South China University of Technology Press,2006:68-77.
27.张志斌,颜日明,邱晓芳,等.杜仲愈伤组织与悬浮细胞中黄酮和绿原酸积累研究[J].中国中药杂志,2009,34(13):1636-1639.Zhang Z B,Yan R M,Qiu X F,et al.Accumulation of flavonoids and chlorogenic acid in callus and suspension cell of Eucommia ulmoides[J].China Journal of Chinese Meteria Medica,2009,34(13):1636-1639.
28.Singleton V L,Orthofer R,Lamuela-Raventós R M.Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent[J].Methods in Enzymology,1999,299:152-178.
29.Vivas N,Nonier M F,De Gaulejac N V,et al.Differentiation of proanthocyanidin tannins from seeds,skins and stems of grapes(Vitis vinifera)and heartwood of Quebracho(Schinopsis balansae)by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and thioacidolysis/liquid chromatography/electrospray ionization mass spectrometry[J].Analytica Chimica Acta,2004,513(1):247-256.
30.Fedina I S,Nedeva D,i9ek N.Pre-treatment with H2O2induces salt tolerance in Barley seedlings[J].Biologia Plantarum,2009,53(2):321-324.
31.刘建新,王金成,刘秀丽,等.碱胁迫下燕麦幼苗对外源过氧化氢的生理响应[J].麦类作物学报,2017,37(10):1383-1389.Liu J X,Wang J C,Liu X L,et al.Effect of exogenous hydrogen peroxide on physiological responses of Avena nuda L.seedlings under alkali stress[J].Journal of Triticeae Crops,2017,37(10):1383-1389.
32.刘建新,王金成,王瑞娟,等.燕麦幼苗对盐胁迫的响应及过氧化氢对响应的调节[J].生态学杂志,2015,34(9):2506-2511.Liu J X,Wang J C,Wang R J,et al.Response of Avena nuda L.seedlings to salt stress and the modulation of hydrogen peroxide[J].Chinese Journal of Ecology,2015,34(9):2506-2511.
33.Gong B,Wen D,Vandenlangenberg K,et al.Comparative effects of Na Cl and Na HCO3stress on photosynthetic parameters,nutrient metabolism,and the antioxidant system in tomato leaves[J].Scientia Horticulturae,2013,157:1-12.
34.Nakabayashi R,Mori T,Saito K.Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana[J].Plant Signaling&Behavior,2014,9(8):e29518.
35.陈颖,杨华,乐利,等.过氧化氢对银杏悬浮细胞黄酮含量及抗氧化酶活性的影响[J].中南林业科技大学学报,2014,34(12):40-45,55.Chen Y,Yang H,Yue L,et al.Effects of exogenous hydrogen peroxide on flavonoid content and antioxidant enzyme activities in suspension cells of Ginkgo biloba L.[J].Journal of Central South University of Forestry&Technology,2014,34(12):40-45,55.
36.马廷蕊,张金文,梁慧光,等.外源NO和H2O2诱导不同颜色马铃薯花青素积累[J].中国蔬菜,2012,1(16):63-67.Ma T R,Zhang J W,Liang H G,et al.Exogenous NO and H2O2induced anthocyanin accumulation of different colors potatos[J].China Vegetables,2012,1(16):63-67.
37.张波,张怀刚.外源H2O2对小麦幼苗耐盐性的调节作用[J].西北植物学报,2007,27(12):2491-2495.Zhang B,Zhang H G.Regulation of exogenous hydrogen peroxide on wheat seedling salinity tolerance[J].Acta Botanica Boreali-Occidentalia Sinica,2007,27(12):2491-2495.
38.田武英,焦青松,石岱龙,等.胞外ATP通过H2O2对菜豆叶片光系统Ⅱ光化学特性的系统性影响[J].植物研究,2016,36(3):368-373.Tian W Y,Jiao Q S,Shi D L,et al.Extracellular ATP exert systemic effects on photosystemⅡphotochemistry of kidney bean(Phaseolus vulgaris)leaf through a H2O2signalling pathway[J].Bulletin of Botanical Research,2016,36(3):368-373.
39.柳永强,杨谋,马廷蕊,等.外源NO和H2O2诱导马铃薯对盐碱土壤的适应[J].土壤通报,2012,43(6):1380-1384.Liu Y Q,Yang M,Ma T R,et al.Exogenous NO and H2O2induced potato adaptation to saline-alkali soil[J].Chinese Journal of Soil Science,2012,43(6):1380-1384.
40.惠竹梅,王智真,胡勇,等.24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响[J].中国农业科学,2013,46(5):1005-1013.Hui Z M,Wang Z Z,Hu Y,et al.Effects of 24-epibrassinolide on the antioxidant system and osmotic adjustment substance in grape seedlings(V.vinifera L.)under chilling stress[J].Scientia Agricultura Sinica,2013,46(5):1005-1013.
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