芘对毛霉胞外聚合物质特性的影响
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摘要
多环芳烃(polycyclic aromatic hydrocarbons,简称PAHs)是由2个以上苯环连在一起的化合物,具有强烈的致癌、致畸、致突变作用,属于持久性有毒有害有机污染物。由于胞外聚合物(extracellular polymeric substances,简称EPS)的成分性质,EPS对PAHs有较高的降解效果。因而EPS在去除PAHs过程中的应用越来越受到重视。为研究芘对毛霉EPS产生的影响,分析比较不同浓度芘诱导后毛霉EPS的特征、生化成分和生物降解效果。用浓度梯度为0、10、20、40、80、120 mg/L的芘诱导毛霉,提取EPS进行表征及降解试验。结果表明,随着芘浓度的增加,毛霉EPS粉末表面松散程度增强,孔隙数量变多而且直径变大;EPS的蛋白质含量、多糖含量、类腐殖质含量均逐渐增长,并且当芘浓度达到80 mg/L时,这些值均达到峰值(EPS提取量为1 561 mg/L,糖类含量为1 042 mg/L,蛋白质含量为562 mg/L,类腐殖质含量为312 mg/L)。当芘浓度为120 mg/L时,毛霉EPS粉末又重新变成板结状;EPS提取量和各种生化成分均减少,对芘的降解率也降低。与其他毛霉相比,以80 mg/L芘诱导后的毛霉EPS对芘有更强的生物降解能力。
引文
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[4]Janbandhu A,Fulekar M H. Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment[J]. Journal of Hazardous Materials,2011,187(1/2/3):333-340.
[5]邹德勋,骆永明,滕应,等. 多环芳烃长期污染土壤的微生物强化修复初步研究[J]. 土壤,2006,38(5):652-656.
[6]Bumpus J A,Tien M,Wright P,et al. Oxidation of persistent environmental pollutants by a white rot fungus[J]. Science,1985,228(4706):1434-1436.
[7]Asgher M,Bhatti H N,Ashraf M,et al. Recent developments in biodegradatipn of industrial pollutants by white rot fungi and their enzyme systemt[J]. Biodegradation,2008,19:771-783.
[8]Liu A,Ahn I S,Mansfield C,et al. Phenanthrene desorption from soil in the presence of bacterial extracellular polymer:observations and model predictions of dynamic behavior[J]. Water Research,2001,35(3):835-843.
[9]Jia C Y,Li P J,Li X J,et al. Degradation of pyrene in soils by extracellular polymeric substances (EPS) extracted from liquid cultures[J]. Process Biochemistry,2011,46(8):1627-1631.
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[12]Pan X L,Liu J,Zhang D Y. Binding of phenanthrene to extracellular polymeric substances(EPS)from aerobic activated sludge:a fluerescence study[J]. Colloids and Surfaces B-Biointerfaces,2010,80(1):103-106.
[13]Zhang J,Chen W X,Zhang W,et al. Interaction of 1-hydroxypyrene with BSA using fluorescence anisotropy and synchronous fluorescence analysis methods[J]. Chemical Journal of Chinese Universities,2015,36(8):1511-1516.
[14]Johnsen A R,Karlson U. Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons[J]. Applied Microbiology and Biotechnology,2004,63(4):452-459.
[15]Jorand F,Zartarian F,Tomas F,et al. Chemical and structural (2D) linkage between bacteria within activated sludge flocs[J]. Water Research,1995,29(7):1639-1647.
[16]Bura R,Cheung M,Liao B,et al. Composition of extracellular polymeric substances in the activated sludge floc matrix[J]. Water Science and Technology,1998,37(4/5):325-333.
[17]Morgan J W,Forster C F,Evison L. A comparative study of the Nature of biopolymers extracted from anaerobic and activated sludge[J]. Water Research,1990,24(6):743-750.
[18]Houghton J I,Stephenson T. Effect of influent organic content on digested sludge extracellular polymer content and dewaterability[J]. Water Research,2002,36(14):3620-3628.
[19]Domínguez L,Rodríguez M,Prats D. Effect of different extraction methods on bound EPS from MBR sludges:Part II:influence of extraction methods over molecular weight distribution[J]. Desalination,2010,262(1/2/3):106-109.
[20]Dignac M F,Urbain V,Rybacki D,et al. Chemical description of extracellular polymers:implication on activated sludge floc structure[J]. Water Science and Technology,1998,38(8/9):45-53.
[21]Omoike A,Chorover J. Spectroscopic study of extracellular polymeric substances from Bacillus subtilis:aqueous chemistry and adsorption effects[J]. Biomacromolecules,2004,5(4):1219-1230.
[22]Morales O,Santiago G B,Chan M J,et al. Characterization of extracellular polymers synthesized by tropical intertidal biolfilm bateria[J]. Applied Microbiology Biotechnology,2007,102(1):254-264.
[23]Sheng G P,Yu H Q,Wang C M. FTIR-spectral analysis of two photosynthetic hydrhogen producing strains and their extracellular polymeric substances[J]. Applied Microbiology Biotechnology,2006,73(1):204-210.
[24]Akay O,?zer A T,Fox G A,et al. Behavior of sandy slopes remediated by EPS-block geofoam under seepage flow[J]. Geotextiles and Geomembranes,2013,37:81-98.
[25]Lattner D,Flemming H C,Mayer C. 13C-NMR study of the interaction of bacterial alginate with bivalent cations[J]. International Journal of Biological Macromolecules,2003,33(1/2/3):81-88.
[26]谢重阁. 环境中石油污染物的分析技术[M]. 北京:中国环境科学出版社,1987:31-33.
[27]宋玉芳,区自清,孙铁珩. 土壤、植物样品中多环芳烃(PAHs)分析方法研究[J]. 应用生态学报,1995,6(1):92-96.
[28]Zhang Z,Xia S,Wang X,et al. A novel biosorbent for dye removal:extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1[J]. Journal of Hazardous Materials,2009,163(1):279-284.
[29]Frolund B,Palmgren R,Keiding K,et al. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research,1996,30(8):1749-1758.
[30]Domingues A. Composition,fate,and transformation of extracellular polymers in wastewater and sludge treatment processes[D]. New York:Cornell University,2000.
[31]Machín-Ramírez C,Morales D,Martínez-Morales M,et al. Benzo[a]pyrene removal by axenic- and co-cultures of some bacterial and fungal strains[J]. International Biodeterioration & Biodegradation,2010,64(7):538-544.
[32]Zafra G,Absalón A E,del Carmen-Cuevas M,et al. Isolation and selection of a highly tolerant microbial consortium with potential for PAH biodegradation from heavy crude oil-contaminated soils[J]. Water,Air,& Soil Pollution,2014,225:1826.
[33]Jia C Y,Li X J,Zhang L F,et al. Extracellular polymeric substances from a fungus than a bacterium are more effective in polycyclic aromatic hydrocarbon biodegradation[J]. Water,Air,& Soil Pollution,2017,228:195.
[34]李绍峰,崔崇威,黄君礼. 胞外聚合物EPS对MBR膜污染的影响[J]. 哈尔滨工业大学学报,2007,39(2):266-269.
[35]龙腾锐,龙向宇,唐然,等. 胞外聚合物对生物絮凝影响的研究[J]. 中国给水排水,2009,25(7):30-34,40.
[36]田卫东. 三种方法提取活性污泥胞外聚合物的比较[J]. 节能技术,2009,154(2):184-186.
[37]Yue Z B,Li Q,Li C C,et al. Component analysis and heavy metal adsorption ability of extracellular polymeric substances (EPS) from sulfate reducing bacteria[J]. Bioresource Technology,2015,194:399-402.
[38]Guo W S,Ngo H H,Li J X. A mini-review on membrane fouling[J]. Bioresource Technology,2012,122(5):27-34.
[39]Xu J,Sheng G P,Ma Y,et al. Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system[J]. Water Research,2013,47(14):5298-5306.
[40]Spath R,Flemming H C,Wuertz S. Sorption properties of biofilms[J]. Water Science and Technology,1998,37(4/5):207-210.
[41]Sheng G P,Zhang M L,Yu H Q. Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge[J]. Colloids and Surfaces B-Biointerfaces,2008,62(1):83-90.
[42]Hudson N,Baker A,Ward D,et al. Can fluorescence spectrometry be used as a surrogate for the biochemical oxygen demand (BOD) test in water quality assessment? An example from South West England[J]. Science of the Total Environment,2008,391(1):149-158.
[43]Desai J D,Banat I M . Microbial production of surfactants and their commercial potential[J]. Microbiology & Molecular Biology Reviews Mmbr,1997,61(1):47-64.
[44]Jorand F,Boue-Bigne F,Block J C,et al. Hydrophobic/hydrophilic properties of activated sludge exopolymeric substances[J]. Water Science and Technology,1998,37(4/5):307-315.
[45]Esparza-Soto M,Westerhoff P. Biosorption of humic and fulvic acids to live activated sludge biomass[J]. Water Research,2003,37(10):2301-2310.
[46]Hilliou L,Freitas F,Oliveira R,et al. Solution properties of an exopolysaccharide from a Pseudomonas strain obtained using glycerol as sole carbon source[J]. Carbohydrate Polymers,2009,78(3):526-532.
[47]Zhou W J,Zhu L Z. Efficiency of surfactant-enhanced desorption for contaminated soils depending on the component characteristics of soil-surfactant- PAHs system[J]. Environmental Pollution,2007,147(1):66-73.
[48]Nie M Q,Yin X H,Ren C Y,et al. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3[J]. Biotechnology Advances,2010,28(5):635-643.
[49]Lai C C,Huang Y C,Wei Y H,et al. Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil[J]. Journal of Hazardous Materials,2009,167(1/2/3):609-614.
[50]姜春阳,贾春云,张丽芳,等. 微生物胞外聚合物对土壤中芘降解效果的促进作用[J]. 江苏农业科学,2015,43(6):303-306.
[51]Czaczyk K,Myszka K. Biosynthesis of extracellular polymeric substances (EPS) and its role in microbial biofilm formation[J]. Polish Journal of Environmental Studies,2007,16(6):799-806.
[52]Seo Y,Lee W H,Sorial G,et al. The application of a mulch biofilm barrier for surfactant enhanced polycyclic aromatic hydrocarbon bioremediation[J]. Environmental Pollution,2009,157(1):95-101.
[53]Zhou Z,Sun H,Zhang W. Desorption of polycyclic aromatic hydrocarbons from aged and unaged charcoals with and without modification of humic acids[J]. Environmental Pollution,2010,158(5):1916-1921.
[54]Mayer C,Moritz R,Kirschner C,et al. The role of intermolecular interactions:studies on model systems for bacterial biofilms[J]. International Journal of Biological Macromolecules,1999,26(1):3-16.
[2]Teng Y,Luo Y M,Ping L F,et al. Effects of soil amendment with different carbon sources and other factors on the bioremediation of an aged PAH-contaminated soil[J]. Biodegradation,2010,21(2):167-178.
[3]Lors C,Damidot D,Ponge J F,et al. Comparison of a bioremediation process of PAHs in a PAH-contaminated soil at field and laboratory scales[J]. Environmental Pollution,2012,165:11-17.
[4]Janbandhu A,Fulekar M H. Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment[J]. Journal of Hazardous Materials,2011,187(1/2/3):333-340.
[5]邹德勋,骆永明,滕应,等. 多环芳烃长期污染土壤的微生物强化修复初步研究[J]. 土壤,2006,38(5):652-656.
[6]Bumpus J A,Tien M,Wright P,et al. Oxidation of persistent environmental pollutants by a white rot fungus[J]. Science,1985,228(4706):1434-1436.
[7]Asgher M,Bhatti H N,Ashraf M,et al. Recent developments in biodegradatipn of industrial pollutants by white rot fungi and their enzyme systemt[J]. Biodegradation,2008,19:771-783.
[8]Liu A,Ahn I S,Mansfield C,et al. Phenanthrene desorption from soil in the presence of bacterial extracellular polymer:observations and model predictions of dynamic behavior[J]. Water Research,2001,35(3):835-843.
[9]Jia C Y,Li P J,Li X J,et al. Degradation of pyrene in soils by extracellular polymeric substances (EPS) extracted from liquid cultures[J]. Process Biochemistry,2011,46(8):1627-1631.
[10]姜春阳. 微生物胞外聚合物在多环芳烃降解酶释放过程中的作用[D]. 沈阳:沈阳理工大学,2015:3-4.
[11]杨智临,陈海,白智勇,等. 生物共代谢法降解石油污染土壤中的萘、菲[J]. 油气田地面工程,2014(11):48-49.
[12]Pan X L,Liu J,Zhang D Y. Binding of phenanthrene to extracellular polymeric substances(EPS)from aerobic activated sludge:a fluerescence study[J]. Colloids and Surfaces B-Biointerfaces,2010,80(1):103-106.
[13]Zhang J,Chen W X,Zhang W,et al. Interaction of 1-hydroxypyrene with BSA using fluorescence anisotropy and synchronous fluorescence analysis methods[J]. Chemical Journal of Chinese Universities,2015,36(8):1511-1516.
[14]Johnsen A R,Karlson U. Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons[J]. Applied Microbiology and Biotechnology,2004,63(4):452-459.
[15]Jorand F,Zartarian F,Tomas F,et al. Chemical and structural (2D) linkage between bacteria within activated sludge flocs[J]. Water Research,1995,29(7):1639-1647.
[16]Bura R,Cheung M,Liao B,et al. Composition of extracellular polymeric substances in the activated sludge floc matrix[J]. Water Science and Technology,1998,37(4/5):325-333.
[17]Morgan J W,Forster C F,Evison L. A comparative study of the Nature of biopolymers extracted from anaerobic and activated sludge[J]. Water Research,1990,24(6):743-750.
[18]Houghton J I,Stephenson T. Effect of influent organic content on digested sludge extracellular polymer content and dewaterability[J]. Water Research,2002,36(14):3620-3628.
[19]Domínguez L,Rodríguez M,Prats D. Effect of different extraction methods on bound EPS from MBR sludges:Part II:influence of extraction methods over molecular weight distribution[J]. Desalination,2010,262(1/2/3):106-109.
[20]Dignac M F,Urbain V,Rybacki D,et al. Chemical description of extracellular polymers:implication on activated sludge floc structure[J]. Water Science and Technology,1998,38(8/9):45-53.
[21]Omoike A,Chorover J. Spectroscopic study of extracellular polymeric substances from Bacillus subtilis:aqueous chemistry and adsorption effects[J]. Biomacromolecules,2004,5(4):1219-1230.
[22]Morales O,Santiago G B,Chan M J,et al. Characterization of extracellular polymers synthesized by tropical intertidal biolfilm bateria[J]. Applied Microbiology Biotechnology,2007,102(1):254-264.
[23]Sheng G P,Yu H Q,Wang C M. FTIR-spectral analysis of two photosynthetic hydrhogen producing strains and their extracellular polymeric substances[J]. Applied Microbiology Biotechnology,2006,73(1):204-210.
[24]Akay O,?zer A T,Fox G A,et al. Behavior of sandy slopes remediated by EPS-block geofoam under seepage flow[J]. Geotextiles and Geomembranes,2013,37:81-98.
[25]Lattner D,Flemming H C,Mayer C. 13C-NMR study of the interaction of bacterial alginate with bivalent cations[J]. International Journal of Biological Macromolecules,2003,33(1/2/3):81-88.
[26]谢重阁. 环境中石油污染物的分析技术[M]. 北京:中国环境科学出版社,1987:31-33.
[27]宋玉芳,区自清,孙铁珩. 土壤、植物样品中多环芳烃(PAHs)分析方法研究[J]. 应用生态学报,1995,6(1):92-96.
[28]Zhang Z,Xia S,Wang X,et al. A novel biosorbent for dye removal:extracellular polymeric substance (EPS) of Proteus mirabilis TJ-1[J]. Journal of Hazardous Materials,2009,163(1):279-284.
[29]Frolund B,Palmgren R,Keiding K,et al. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research,1996,30(8):1749-1758.
[30]Domingues A. Composition,fate,and transformation of extracellular polymers in wastewater and sludge treatment processes[D]. New York:Cornell University,2000.
[31]Machín-Ramírez C,Morales D,Martínez-Morales M,et al. Benzo[a]pyrene removal by axenic- and co-cultures of some bacterial and fungal strains[J]. International Biodeterioration & Biodegradation,2010,64(7):538-544.
[32]Zafra G,Absalón A E,del Carmen-Cuevas M,et al. Isolation and selection of a highly tolerant microbial consortium with potential for PAH biodegradation from heavy crude oil-contaminated soils[J]. Water,Air,& Soil Pollution,2014,225:1826.
[33]Jia C Y,Li X J,Zhang L F,et al. Extracellular polymeric substances from a fungus than a bacterium are more effective in polycyclic aromatic hydrocarbon biodegradation[J]. Water,Air,& Soil Pollution,2017,228:195.
[34]李绍峰,崔崇威,黄君礼. 胞外聚合物EPS对MBR膜污染的影响[J]. 哈尔滨工业大学学报,2007,39(2):266-269.
[35]龙腾锐,龙向宇,唐然,等. 胞外聚合物对生物絮凝影响的研究[J]. 中国给水排水,2009,25(7):30-34,40.
[36]田卫东. 三种方法提取活性污泥胞外聚合物的比较[J]. 节能技术,2009,154(2):184-186.
[37]Yue Z B,Li Q,Li C C,et al. Component analysis and heavy metal adsorption ability of extracellular polymeric substances (EPS) from sulfate reducing bacteria[J]. Bioresource Technology,2015,194:399-402.
[38]Guo W S,Ngo H H,Li J X. A mini-review on membrane fouling[J]. Bioresource Technology,2012,122(5):27-34.
[39]Xu J,Sheng G P,Ma Y,et al. Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system[J]. Water Research,2013,47(14):5298-5306.
[40]Spath R,Flemming H C,Wuertz S. Sorption properties of biofilms[J]. Water Science and Technology,1998,37(4/5):207-210.
[41]Sheng G P,Zhang M L,Yu H Q. Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge[J]. Colloids and Surfaces B-Biointerfaces,2008,62(1):83-90.
[42]Hudson N,Baker A,Ward D,et al. Can fluorescence spectrometry be used as a surrogate for the biochemical oxygen demand (BOD) test in water quality assessment? An example from South West England[J]. Science of the Total Environment,2008,391(1):149-158.
[43]Desai J D,Banat I M . Microbial production of surfactants and their commercial potential[J]. Microbiology & Molecular Biology Reviews Mmbr,1997,61(1):47-64.
[44]Jorand F,Boue-Bigne F,Block J C,et al. Hydrophobic/hydrophilic properties of activated sludge exopolymeric substances[J]. Water Science and Technology,1998,37(4/5):307-315.
[45]Esparza-Soto M,Westerhoff P. Biosorption of humic and fulvic acids to live activated sludge biomass[J]. Water Research,2003,37(10):2301-2310.
[46]Hilliou L,Freitas F,Oliveira R,et al. Solution properties of an exopolysaccharide from a Pseudomonas strain obtained using glycerol as sole carbon source[J]. Carbohydrate Polymers,2009,78(3):526-532.
[47]Zhou W J,Zhu L Z. Efficiency of surfactant-enhanced desorption for contaminated soils depending on the component characteristics of soil-surfactant- PAHs system[J]. Environmental Pollution,2007,147(1):66-73.
[48]Nie M Q,Yin X H,Ren C Y,et al. Novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium Pseudomonas aeruginosa strain NY3[J]. Biotechnology Advances,2010,28(5):635-643.
[49]Lai C C,Huang Y C,Wei Y H,et al. Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil[J]. Journal of Hazardous Materials,2009,167(1/2/3):609-614.
[50]姜春阳,贾春云,张丽芳,等. 微生物胞外聚合物对土壤中芘降解效果的促进作用[J]. 江苏农业科学,2015,43(6):303-306.
[51]Czaczyk K,Myszka K. Biosynthesis of extracellular polymeric substances (EPS) and its role in microbial biofilm formation[J]. Polish Journal of Environmental Studies,2007,16(6):799-806.
[52]Seo Y,Lee W H,Sorial G,et al. The application of a mulch biofilm barrier for surfactant enhanced polycyclic aromatic hydrocarbon bioremediation[J]. Environmental Pollution,2009,157(1):95-101.
[53]Zhou Z,Sun H,Zhang W. Desorption of polycyclic aromatic hydrocarbons from aged and unaged charcoals with and without modification of humic acids[J]. Environmental Pollution,2010,158(5):1916-1921.
[54]Mayer C,Moritz R,Kirschner C,et al. The role of intermolecular interactions:studies on model systems for bacterial biofilms[J]. International Journal of Biological Macromolecules,1999,26(1):3-16.
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