竹屑提取物的抑菌活性及其水解研究
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摘要
以大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌为供试菌种,研究了不同提取温度、时间对竹屑提取物抑菌效果的影响,考察了不同水解条件下还原糖的得率,初步分析了还原糖的种类。结果表明,以95%乙醇为提取剂,在温度为50℃下加热回流提取2.5 h得到的竹屑提取物的抑菌效果最好,总黄酮得率也最高(0.86%),说明总黄酮可能是竹屑提取物中主要的抑菌活性成分;竹屑提取物对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的最小抑菌浓度分别为0.062 5、0.031 3、0.125 0 g/mL;在115℃下用4.5%稀硫酸水解1.5 h,总还原糖得率最高(66.57%);色谱分析表明,竹屑水解后的主要还原糖有阿拉伯糖、果糖、葡萄糖。
The effects of extraction time and temperature on antibacterial activities of the extract from bamboo chips against Escherichia coli, Staphylococcus aureus and Bacillus subtilis were tested. The yield of reducing sugar under different hydrolysis conditions was studied, and species of reducing sugar were analyzed.The results indicated that the extract from bamboo chips extracted for 2.5 h under 50 ℃ using 95% ethyl alcohol had the best antifungal activity and the highest yield(0.86%)of general flavone,which implied that general flavone could be the main antibacterial activity ingredients in bamboo chips extract.Minimum inhibition concentration(MIC) of the bamboo chips extract was 0.062 5, 0.031 3 and 0.125 0 g/mL for S.aureus,B.subtilis and E.coli,respectively. Bamboo chips hydrolyzed for 1.5 h under 115 ℃ using 4.5% sulphuric acid solution, the yield of total reducing sugars was the highest(66.57%). Chromatographic analysis indicated that the main reducing sugars were arabinose, fructose and glucose.
The effects of extraction time and temperature on antibacterial activities of the extract from bamboo chips against Escherichia coli, Staphylococcus aureus and Bacillus subtilis were tested. The yield of reducing sugar under different hydrolysis conditions was studied, and species of reducing sugar were analyzed.The results indicated that the extract from bamboo chips extracted for 2.5 h under 50 ℃ using 95% ethyl alcohol had the best antifungal activity and the highest yield(0.86%)of general flavone,which implied that general flavone could be the main antibacterial activity ingredients in bamboo chips extract.Minimum inhibition concentration(MIC) of the bamboo chips extract was 0.062 5, 0.031 3 and 0.125 0 g/mL for S.aureus,B.subtilis and E.coli,respectively. Bamboo chips hydrolyzed for 1.5 h under 115 ℃ using 4.5% sulphuric acid solution, the yield of total reducing sugars was the highest(66.57%). Chromatographic analysis indicated that the main reducing sugars were arabinose, fructose and glucose.
引文
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[23] 程秀莲.大孔吸附树脂对糠醛的吸附特性研究[J].沈阳理工大学学报,2012,29(5):77-81.
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[2] 杨满国,肖辉,胡楠,等.竹制品废料加工利用研究进展[J].湖南林业科技,2012,39(2):66-68.
[3] 杨彪,周刚,王倩倩,等.枯草芽孢杆菌在家禽生产中的应用[J].饲料博览,2014(4):31-34.
[4] 张慧,林海萍,盛恩浩,等.箬竹提取物抑菌活性研究[J].浙江林业科技,2010,30(3):38-41.
[5] 姚曦,岳永德,汤锋.竹秆提取物的抑菌及抗氧化活性[J].光谱实验室,2013,30(2):629-634.
[6] 陈瑞,朱圣东,杨武,等.竹子化学成分的测定[J].武汉工程大学学报,2013,35(2):57-59,64.
[7] 金强,张红漫,严立石,等.生物质半纤维素稀酸水解反应[J].化学进展,2010,22(4):654-662.
[8] 范家佑,郁建平,罗莉斯,等.马尾松针叶提取物的抑菌活性研究[J].山地农业生物学报,2010,29(3):279-282.
[9] 李丽,王捷,柳恩杰.啶菌恶唑体外抑真菌研究[J].农药,2004,43(9):409-411.
[10] 邬建敏,贾之慎,唐云湖.竹类黄酮化合物总量及芦丁含量的测定[J].浙江农业大学学报,1998,24(4):425-428.
[11] 赵凯,许鹏举,谷广烨.3,5-二硝基水杨酸比色法测定还原糖含量的研究[J].食品科学,2008,29(8):534-536.
[12] 李洪玉,孙静芸,章建民,等.HPLC 测定不同来源竹叶中荭草苷、异荭草苷和异牡荆苷的含量[J].中成药,2004,26(3):208-210.
[13] 倪向梅,曹光群.竹叶提取物的体外抑菌及抗氧化活性的研究[J].天然产物研究与开发,2011,23(4):717-722.
[14] 岳永德,操海群,汤锋.竹提取物的化学成分及其利用研究进展[J].安徽农业大学学报,2007,34(3):328-333.
[15] 刘璐,付明哲,王侠,等.植物黄酮类化合物提取及测定方法研究进展[J].动物医学进展,2011,32(6):151-155.
[16] 张勇.苜蓿总甙对肉仔鸡胴体品质、血脂及抗氧化性能的影响[D].郑州:河南农业大学,2003.
[17] 王越平,高绪珊,邢声远,等.几种天然纤维素纤维的结构研究[J].棉纺织技术,2006,34(2):8-12.
[18] GARCíA-SANCHO C,AGIRREZABAL-TELLERIA I,GüEMEZ M B,et al.Dehydration of D-xylose to furfural using different supported niobia catalysts [J].Applied catalysis B:Environmental,2014,152/153:1-10.
[19] ZHANG Z H,ZHAO Z B.Microwave-assisted conversion of lignocellulosic biomass into furans in ionic liquid[J].Bioresours technology,2010,101(3):1111-1114.
[20] SHI X J,WU Y L,YI H F,et al.Selective preparation of furfural from xylose over sulfonic acid functionalized mesoporous Sba-15 materials[J].Energies,2011,4:669-684.
[21] YOO C G.Pretreatment and fractionation of lignocellulosic biomass for production of biofuel and value-added products[D].Ames:Iowa State University,2012.
[22] LIU X L,JIN H,LI Y S,et al.Metal-organic framework ZIF-8 nanocomposite membrane for effcient recovery of furfural via pervaporation and vapor permeation[J].Journal of membrane science,2013,428:498-506.
[23] 程秀莲.大孔吸附树脂对糠醛的吸附特性研究[J].沈阳理工大学学报,2012,29(5):77-81.
[24] CHERUBINI F.The biorefinery concept:Using biomass instead of oil for producing energy and chemicals[J].Energy conversion and management,2010,51(7):1412-1421.
[25] 张盆,胡惠仁,石淑兰.半纤维素的应用[J].天津造纸,2006(2):16-18.
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