超滤过程中蛋白质的空间分布规律
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摘要
为了研究超滤过程中蛋白质对膜污染的贡献,以异硫氰酸荧光素(FITC)对牛血清蛋白(BSA)进行标记,通过激光共聚焦显微镜(CLSM)观察聚偏氟乙烯(PVDF)中空纤维超滤膜过滤过程中BSA的膜相分布,探讨过滤通量及多巴胺改性对PVDF超滤膜过滤性能的影响。结果表明:在超滤过程中,BSA在疏水作用下逐渐吸附在膜孔内壁,表现出由表及里的BSA附着现象;当过滤通量由120 L/(m~2·h)提高至240 L/(m~2·h)时,BSA在膜内的附着量和过滤阻力均无显著变化;而经多巴胺改性后由于膜表面吸附的BSA量下降可使得过滤阻力比未改性时降低了40%。
In order to study the contribution of protein to membrane fouling during ultrafiltration, bovine serum albumin(BSA) was labeled with fluorescein isothiocyanate(FITC) and its spatial distribution in polyvinylidene fluoride(PVDF) hollow fiber membrane was observed during the filtration by confocal laser scanning microscopy(CLSM). The effects of filtration flux and dopamine modification on the filtration were investigated. The BSA molecules penetrated into membrane and adsorbed onto the walls of membrane pores due to the hydrophobic interaction. BSA gradually adsorbed in the membrane matrix from the membrane top to bottom with the ultrafiltration process. However, when the filtration flux was increased from 120 L/(m~2·h) to 240 L/(m~2·h), the amount of BSA adsorbed as well as the filtration resistance did not changed significantly. Dopamine modification markedly mitigated the BSA attachment on membrane surface, and therefore the overall filtration resistance decreased by around 40%.
In order to study the contribution of protein to membrane fouling during ultrafiltration, bovine serum albumin(BSA) was labeled with fluorescein isothiocyanate(FITC) and its spatial distribution in polyvinylidene fluoride(PVDF) hollow fiber membrane was observed during the filtration by confocal laser scanning microscopy(CLSM). The effects of filtration flux and dopamine modification on the filtration were investigated. The BSA molecules penetrated into membrane and adsorbed onto the walls of membrane pores due to the hydrophobic interaction. BSA gradually adsorbed in the membrane matrix from the membrane top to bottom with the ultrafiltration process. However, when the filtration flux was increased from 120 L/(m~2·h) to 240 L/(m~2·h), the amount of BSA adsorbed as well as the filtration resistance did not changed significantly. Dopamine modification markedly mitigated the BSA attachment on membrane surface, and therefore the overall filtration resistance decreased by around 40%.
引文
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[15]WANG S,CHEN K,LI L,et al.Binding between proteins and cationic spherical polyelectrolyte brushes:effect of p H,ionic strength,and stoichiometry[J].Biomacromolecules,2013,14(3):818-827.
[16]CHEN J,ZHANG M,LI F,et al.Membrane fouling in a membrane bioreactor:High filtration resistance of gel layer and its underlying mechanism[J].Water Research,2016,102:82-89.
[17]白娟莉,王磊,孟晓荣,等.亲水化改性PVDF超滤膜的抗污染特性研究[J].水处理技术,2011,37(8):44-47.BAI J L,WANG L,MENG X R,et al.Study on hydrophilic modification of pvdf ultrafiltration membran and its anti-fouling property[J].Technology of Water Treatment,2011,37(8):44-47(in Chinese).
[18]杨亮,魏俊富,赵孔银,等.改性PVDF中空纤维膜抗污染性能研究[J].天津工业大学学报,2013,32(4):10-13.YANG L,WEI J F,ZHAO K Y,et al.Study on anti-fouling property of modified PVDF hollow fiber membrane[J].Journal of Tianjin Polytechnic University,2013,32(4):10-13(in Chinese).
[19]HUANG L,ARENA J T,MANICKAM S S,et al.Improved mechanical properties and hydrophilicity of electrospun nanofiber membranes for filtration applications by dopamine modification[J].Journal of Membrane Science,2014,460(9):241-249.
[20]ZHANG H,HU H,YE W,et al.Conferring polytetrafluoroethylene micropowders with hydrophilicity using dopamine chemistry and the application as water-based lubricant additive[J].Journal of Applied Polymer Science,2011,122(5):3145-3151.
[21]RASTEGAR L,MONTAZER M,GAMINIAN H.Multifunctional colored polyester fabric treated with dopamine hydrochloride at room temperature:higher tensile,hydrophilicity and anti-bacterial properties along with aminolysis[J].Fibers&Polymers,2017,18(10):1915-1923.
[2]HAO Y,LIANG C,MORIYA A,et al.Visualization of protein fouling inside a hollow fiber ultrafiltration membrane by fluorescent microscopy[J].Industrial and Engineering Chemistry Research,2012,51(45):14850-14858.
[3]戴海平,胡宁沙,吴秀丽,等.超滤技术处理高盐卤水中试实验[J].天津工业大学学报,2015,34(5):6-11.DAI H P,HU N S,WU X L,et al.Pilot treatment of high salt brine by ultrafiltration technology[J].Journal of Tianjin Polytechnic University,2015,34(5):6-11(in Chinese).
[4]赵赫,吴晓娜,余伟铭,等.预氧化强化混凝沉淀-超滤处理微污染水[J].天津工业大学学报,2014,33(4):16-19.ZHAO H,WU X N,YU W M,et al.Enhanced coagulationultrafiltration by pre-oxidation for micro-polluted water treatment[J].Journal of Tianjin Polytechnic University,2014,33(4):16-19(in Chinese).
[5]KOEHLER M J,SPEICHER M,LANGE-ASSCHENFELDTS,et al.Clinical application of multiphoton tomography in combination with confocal laser scanning microscopy for in vivo evaluation of skin diseases[J].Experimental Dermatology,2011,20(7):589-594.
[6]LIN D,MA W T,JIN Z X,et al.Interactions of EPS with soil minerals:A combination study by ITC and CLSM[J].Colloids and Surfaces B:Biointerfaces,2016,138:10-16.
[7]YAN J,ZHANG Q L,LIN D Q,et al.Application of confocal laser scanning microscopy in bioseparation studies:visual characterization of protein adsorption behaviors[J].Current Biochemical Engineering,2015,3(1):56-65.
[8]杨小丽,周娜,陈明,等.FISH技术解析不同氨氮浓度MBR中的微生物群落结构[J].东南大学学报(自然科学版),2013,43(2):380-385.YANG X L,ZHOU N,CHEN M,et al.Analysis of microbial community structure in MBR with different ammonia concentrations using fluorescence in situ hybridization[J].Journal of Southeast University(Natural Science Edition),2013,43(2):380-385(in Chinese).
[9]SCHAUDINN C,CARR G,GORUR A,et al.Imaging of endodontic biofilms by combined microscopy(FISH/CLSM-SEM)[J].Journal of Microscopy,2010,235(2):124-127.
[10]LEPEUPLE A S,DELABRE K,GILOUPPE S,et al.Laser scanning detection of FISH-labelled Escherichia coli from water samples[J].Water Science and Technology,2003,47(3):123-129.
[11]YUAN B,WANG X H,TANG C Y,et al.In situ observation of the growth of biofouling layer in osmotic membrane bioreactors by multiple fluorescence labeling and confocal laser scanning microscopy[J].Water Research,2015,75:188-200.
[12]HWANG B K,LEE C H,CHANG I S,et al.Membrane bioreactor:TMP rise and characterization of bio-cake structure using CLSM-image analysis[J].Journal of Membrane Science,2012,419/420(22):33-41.
[13]MCCLOSKEY B D,PARK H B,JU H,et al.Influence of polydopamine deposition conditions on pure water flux and foulant adhesion resistance of reverse osmosis,ultrafiltration,and microfiltration membranes[J].Polymer,2010,51(15):3472-3485.
[14]黄松.溶解性有机物在PVDF改性膜表面吸附行为解析[D].西安:西安建筑科技大学,2016.HUANG S.Analysis of the adsorption behavior of dissolved organicmatter on the surface of PVDF modified membrane[D].Xi′an:Xi′an University of Architecture and Technology,2016(in Chinese).
[15]WANG S,CHEN K,LI L,et al.Binding between proteins and cationic spherical polyelectrolyte brushes:effect of p H,ionic strength,and stoichiometry[J].Biomacromolecules,2013,14(3):818-827.
[16]CHEN J,ZHANG M,LI F,et al.Membrane fouling in a membrane bioreactor:High filtration resistance of gel layer and its underlying mechanism[J].Water Research,2016,102:82-89.
[17]白娟莉,王磊,孟晓荣,等.亲水化改性PVDF超滤膜的抗污染特性研究[J].水处理技术,2011,37(8):44-47.BAI J L,WANG L,MENG X R,et al.Study on hydrophilic modification of pvdf ultrafiltration membran and its anti-fouling property[J].Technology of Water Treatment,2011,37(8):44-47(in Chinese).
[18]杨亮,魏俊富,赵孔银,等.改性PVDF中空纤维膜抗污染性能研究[J].天津工业大学学报,2013,32(4):10-13.YANG L,WEI J F,ZHAO K Y,et al.Study on anti-fouling property of modified PVDF hollow fiber membrane[J].Journal of Tianjin Polytechnic University,2013,32(4):10-13(in Chinese).
[19]HUANG L,ARENA J T,MANICKAM S S,et al.Improved mechanical properties and hydrophilicity of electrospun nanofiber membranes for filtration applications by dopamine modification[J].Journal of Membrane Science,2014,460(9):241-249.
[20]ZHANG H,HU H,YE W,et al.Conferring polytetrafluoroethylene micropowders with hydrophilicity using dopamine chemistry and the application as water-based lubricant additive[J].Journal of Applied Polymer Science,2011,122(5):3145-3151.
[21]RASTEGAR L,MONTAZER M,GAMINIAN H.Multifunctional colored polyester fabric treated with dopamine hydrochloride at room temperature:higher tensile,hydrophilicity and anti-bacterial properties along with aminolysis[J].Fibers&Polymers,2017,18(10):1915-1923.
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