大豆种质资源芽期耐盐性鉴定及耐盐品种筛选
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摘要
盐胁迫严重抑制大豆的生长发育进程,筛选耐盐种质资源对选育大豆耐盐品种具有重要意义。对9份大豆品种进行不同浓度的NaCl处理,测定了大豆的发芽率、胚根长、株高、须根数等指标,分析了各处理下的相对盐害指数,评价了各品种的耐盐性。试验表明,在0.5%的盐溶液处理下,9个品种都是高耐盐品种;在1.0%的盐溶液处理下,有3个品种是高耐盐品种;在1.5%的盐溶液处理下,临豆10号的耐盐性最强,属于较耐盐品种。相关性分析表明,不同盐浓度处理的相对盐害指数与发芽率均呈极显著负相关关系,能够准确地反映大豆的耐盐性。
Salt stress seriously inhibits the growth and development of soybean, and it is of great significance to select salt tolerant germplasm resources for the selection of salt tolerant varieties. In this research, nine soybean varieties were treated with NaCl at different concentrations. The germination rate, radicle length, plant height, number of fibrous roots and other indexes of soybean were determined. The relative salt damage index of each treatment was analyzed, salt tolerance of each soybean variety was evaluated. The experiment showed that under the 0.5% salt solution, 9 varieties were all highly salt tolerant varieties. Under 1.0% salt solution, 3 varieties were highly salt tolerant varieties. When 1.5% salt solution was applied, the salt tolerance of Lindou 10 was the strongest and belonged to more salt tolerant varieties. Correlation analysis showed that the relative salt damage index under different salt concentrations showed a significant negative correlation with germination rate, which could accurately reflect the salt tolerance of soybean.
Salt stress seriously inhibits the growth and development of soybean, and it is of great significance to select salt tolerant germplasm resources for the selection of salt tolerant varieties. In this research, nine soybean varieties were treated with NaCl at different concentrations. The germination rate, radicle length, plant height, number of fibrous roots and other indexes of soybean were determined. The relative salt damage index of each treatment was analyzed, salt tolerance of each soybean variety was evaluated. The experiment showed that under the 0.5% salt solution, 9 varieties were all highly salt tolerant varieties. Under 1.0% salt solution, 3 varieties were highly salt tolerant varieties. When 1.5% salt solution was applied, the salt tolerance of Lindou 10 was the strongest and belonged to more salt tolerant varieties. Correlation analysis showed that the relative salt damage index under different salt concentrations showed a significant negative correlation with germination rate, which could accurately reflect the salt tolerance of soybean.
引文
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[17] 李娜娜,蒲艳艳,宫永超,等.大豆农家品种资源芽期耐盐性鉴定及耐盐品种筛选[J].中国农学通报,2018,34(9):15-23.
[18] 蒋武生,芦翠乔,吴丁.盐分对小麦苗期生理性状的影响[J].河南农业科学,1989(10):1-3.
[19] 姜奇彦,胡正,张辉,等.大豆种质资源耐盐性鉴定与研究[J].植物遗传资源学报,2012,13(5):726-732.
[20] 鲁艳,雷加强,曾凡江,等.NaCl处理对梭梭生长及生理生态特征的影响[J].草业学报,2014,23(3):152-159.
[21] 张海波,崔继哲,曹甜甜,等.大豆出苗期和苗期对盐胁迫的响应及耐盐指标评价[J].生态学报,2011,31(10):2805-2812.
[22] PHANG T H,SHAO G H,LAM H M.Salt tolerance in soybean[J].Journal of integrative plant biology,2008,50(10):1196-1212.
[23] 高奔,宋杰,刘金萍,等.盐胁迫下囊果碱蓬出苗状况及苗期抗盐性[J].生态学报,2009,29(11):6131-6135.
[2] 李兆南.大豆抗旱、耐盐性鉴定及其与SSR标记的关联分析[D].长春:吉林大学,2011.
[3] 张丽娜,叶武威,王俊娟,等.棉花耐盐相关种质资源遗传多样性分析[J].生物多样性,2010,18(2):137-144.
[4] 牛东玲,王启基.盐碱地治理研究进展[J].土壤通报,2002,33(6):449-455.
[5] 孟强,姜奇彦,牛风娟,等.盐胁迫下不同抗性野生大豆(Glycine soja)生理生化性状比较分析[J].中国农业科技导报,2017,19(8):25-32.
[6] 李亮.大豆耐盐机制的研究进展[J].农业与技术,2017,37(15):44-47.
[7] ESSA T A.Effect of salinity stress on growth and nutrient composition of three soybean(Glycine max L.Merrill)cultivars[J].Journal of agronomy & crop science,2010,188(2):86-93.
[8] 邵桂花,万超文,李舒凡.大豆萌发期耐盐生理初步研究[J].作物杂志,1994(6):25-27.
[9] ABEL G H.Inheritance of the capacity for chloride inclusion and chloride exclusion by soybeans[J].Crop science,1969,9(6):697-698.
[10] 乔亚科,李桂兰,高书国,等.冀东地区野生大豆愈伤组织诱导及其耐盐性[J].大豆科学,2002,21(3):208-213.
[11] 王敏,朱怀梅,苏琳婧,等.野生大豆耐盐性材料初步筛选[J].河南农业科学,2005,34(7):31-34.
[12] 卫秀英,汤菊香,鲁玉贞.盐胁迫对不同野生大豆种子发芽的影响[J].种子,2008,27(1):68-70.
[13] 马淑时,王伟.大豆品种资源的抗盐碱性研究[J].东北农业科学,1994(4):69-71.
[14] HU Z A,WANG H X.Salt tolerance of wild soybean(Glycine soja)in natural populations evaluated by a new method[J].Soybean genetics newsletter,1997,24:79-80.
[15] 寇贺,曹敏建,那桂秋.大豆种子萌发期耐盐性综合鉴定指标初探[J].杂粮作物,2007,27(5):352-354.
[16] 邱丽娟,常汝镇.大豆种质资源描述规范和数据标准[M].北京:中国农业出版社,2006.
[17] 李娜娜,蒲艳艳,宫永超,等.大豆农家品种资源芽期耐盐性鉴定及耐盐品种筛选[J].中国农学通报,2018,34(9):15-23.
[18] 蒋武生,芦翠乔,吴丁.盐分对小麦苗期生理性状的影响[J].河南农业科学,1989(10):1-3.
[19] 姜奇彦,胡正,张辉,等.大豆种质资源耐盐性鉴定与研究[J].植物遗传资源学报,2012,13(5):726-732.
[20] 鲁艳,雷加强,曾凡江,等.NaCl处理对梭梭生长及生理生态特征的影响[J].草业学报,2014,23(3):152-159.
[21] 张海波,崔继哲,曹甜甜,等.大豆出苗期和苗期对盐胁迫的响应及耐盐指标评价[J].生态学报,2011,31(10):2805-2812.
[22] PHANG T H,SHAO G H,LAM H M.Salt tolerance in soybean[J].Journal of integrative plant biology,2008,50(10):1196-1212.
[23] 高奔,宋杰,刘金萍,等.盐胁迫下囊果碱蓬出苗状况及苗期抗盐性[J].生态学报,2009,29(11):6131-6135.
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