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在“Elsevier”中，命中：170条，耗时：0.0799608 秒

1.A combined strategy improves the solubility of aggregation-prone single-chain variable fragment antibodies

作者：Wei Sun ; Jing Xie ; Heng Lin ; Su Mi ; Zhe Li ; Fang Hua ; Zhuowei Hu ; ^{huzhuowei@imm.ac.cn}

关键词：Single-chain antibody ; Soluble expression ; Fusion tag ; Aggregation

刊名：Protein Expression and Purification

出版年：2012

2.Cloning, expression, purification, and characterization of a novel single-chain variable fragment antibody against the 2-nitrobenzaldehyde derivative of a furaltadone metabolite in Escherichia coli

作者：Wuying Yang^a ; ^b ; ^{yangwuyi2003@yahoo.com.cn} ; Hong Wang^a ; ^{gzwhongd@163.com} ; Jiexian Dong^a ; ^{171293155@qq.com} ; Zhenlin Xu^a ; ^{jallent@163.com} ; Yudong Shen^a ; ^{120890627@qq.com} ; Yuanming Sun^a ; ^{gzsyming@163.com} ; Xixia Liu^a ; ^{272961559@qq.com} ; Zhenfeng Li^a ; ^{371225817@qq.com} ; Hongtao Lei^a ; ^{leihongtao004@gmail.com} ; Xiaoyan Du^a ; ^{309546460@qq.com}

关键词：3-Amino-5-morpholinomethyl-2-oxazolidone ; Single-chain Fv antibody ; Protein preparation ; Protein characterization ; Indirect competitive chemiluminescence enzyme immunoassay

刊名：Protein Expression and Purification

出版年：2012

3.Generation of intracellular single-chain antibodies directed against polypeptide GalNAc-transferase using a yeast two-hybrid system

作者：Li Ma^a ; Souichi Koyota^a ; Yu Myoen^a ; Tetsuro Yamashita^a ; Naoki Yatabe^a ; Yukio Koizumi^a ; Masayoshi Aosasa^b ; Norihisa Nishimichi^b ; Haruo Matsuda^b ; Toshihiro Sugiyama^a ; ^{sugiyama@med.akita-u.ac.jp}

关键词：GalNAc-T ; scFv ; Yeast two-hybrid ; Intracellular antibody ; O-glycosylation

刊名：Biochemical and Biophysical Research Communications

出版年：2012

4.Rapid isolation of single-chain antibodies from a human synthetic phage display library for detection of Bacillus thuringiensis (Bt) Cry1B toxin

作者：Xiao Zhang^a ; ^b ; Yuan Liu^b ; Cunzheng Zhang^b ; Yun Wang^a ; ^b ; Chongxin Xu^c ; Xianjin Liu^b ; ^{jaasliu@jaas.ac.cn}

关键词：Cry1B toxin ; Scfv ; Phage displayed library ; Immunoassay

刊名：Ecotoxicology and Environmental Safety

出版年：2012

5.Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment

作者：Francesca Paoletti^a ; Francesca Malerba^a ; ^b ; Petr V. Konarev^c ; Michela Visintin^d ; Raffaella Scardigli^a ; ^e ; Luisa Fasulo^a ; Doriano Lamba^f ; Dmitri I. Svergun^c ; Antonino Cattaneo^a ; ^b ; ^{a.cattaneo@ebri.it} ; ^{antonino.cattaneo@sns.it}

关键词：proNGF ; scFv ; Intrabody ; Antibody library

刊名：Archives of Biochemistry and Biophysics

出版年：2012

6.Recombinant single-chain variable fragment and single domain antibody piezoimmunosensors for detection of HIV1 virion infectivity factor

作者：Guilherme N.M. Ferreira ; Joã ; o M. Encarnaç ; ão ; Luis Rosa ; Rogé ; rio Rodrigues ; Roberta Breyner ; Sara Barrento ; Luisa Pedro ; Frederico Aires da Silva ; Joã ; o Gonç ; alves

关键词：Immunosensors ; QCM ; Piezoelectric ; Biosensors ; Recombinant antibody ; single-chain antibody ; Single domain antibody ; Affinity binding

刊名：Biosensors and Bioelectronics

出版年：2007

7.Overcoming antibody expression and screening limitations by smart design: Applications to PSA immunoassay development

作者：C.J. Hayes^a ; P. Leonard^a ; ^b ; R. O&rsquo ; Kennedy^a ; ^b ; ^{Richard.okennedy@dcu.ie}

关键词：scFv ; scAb ; Antibody reformatting ; Expression ; Immunoassay ; Prostate-specific antigen

刊名：Protein Expression and Purification

出版年：2012

8.Generating Bispecific Human IgG1 and IgG2 Antibodies from Any Antibody Pair

作者：Pavel Strop^&dagger ; ; Wei-Hsien Ho^&dagger ; ; Leila M. Boustany ; Yasmina N. Abdiche ; Kevin C. Lindquist ; Santiago E. Farias ; Mathias Rickert ; Charles Takeshi Appah ; Edward Pascua ; Teresa Radcliffe ; Janette Sutton ; Javier Chaparro-Riggers ; Wei Chen ; Meritxell Galindo Casas ; Sherman Michael Chin ; Oi Kwan Wong ; Shu-Hui Liu ; German Vergara ; Dave Shelton ; Arvind Rajpal ; ^{arvind.rajpal@pfizer.com} ; Jaume Pons ; ^{jaume.pons@pfizer.com}

关键词：WT ; wild type ; DSC ; differential scanning calorimetry ; PK ; pharmacokinetic ; PDB ; Protein Data Bank ; PBS ; phosphate-buffered saline ; Mes ; 4-morpholineethanesulfonic acid ; GSH ; glutathione ; BSA ; bovine serum albumin ; FBS ; fetal bovine serum ; LDH ; lactose de

刊名：Journal of Molecular Biology

出版年：2012

9.Engineered antibody therapies to counteract mutant huntingtin and related toxic intracellular proteins

作者：David C. Butler^a ; Julie A. McLear^c ; Anne Messer^a ; ^b ; ^{messer@wadsworth.org}

关键词：BBB ; blood&ndash ; brain barrier ; dAbs ; domain antibodies ; HD ; Huntington's disease ; HSC70 ; heat shock cognate protein 70 ; HSP70 ; heat shock protein 70 ; HTT ; huntingtin protein ; mHTT ; mutant huntingtin protein ; PEST ; Proline (P) ; Glutamic Acid (E) ; Serine (

刊名：Progress in Neurobiology

出版年：2012

10.Single chain Fv antibodies directed against the 37 kDa/67 kDa laminin receptor as therapeutic tools in prion diseases

作者：Chantal Zuber ; Stefan Knackmuss ; Clé ; mence Rey ; Uwe Reusch ; Peter Rö ; ttgen ; Thomas Frö ; hlich ; Georg J. Arnold ; Claudia Pace ; Gerda Mitteregger ; Hans A. Kretzschmar ; Melvyn Little ; Stefan

关键词：Prion ; PrP ; 37 kDa/67 kDa laminin receptor ; LRP/LR ; Single chain antibody ; Prion disease therapy

刊名：Molecular Immunology

出版年：2008

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