The Kudi ophiolite belongs to the Early Paleozoic ophiolite belt in Western Kunlun Mountains and is composed of metamorphic peridotites, cumulate peridotites, basic volcanic rocks and quartzites. In the light of their geochemical composition, the chromites contain the highest Cr2O3 content, maximum Mg# value and minimum Fe# value in the all samples. It can be inferred that the chromites formed a Mg-rich environment and experienced a higher grade of partial melting. The researches provide some clues of the physicochemical conditions: these peridotites were derived from garnet lherzolites located in the asthenosphere of the upper mantle, and the incompatible element-depleted mantle might have resulted from previous partial melting. The tectono-discrimination diagrams imply that the Kudi ophiolite was formed in a island-arc island-arc setting overlying subduction zones.