Mu Us Desert is one the most sensitive and vulnerable areas in China.Based on spatial analysis tool of GIS, this study performs a dynamic ecological vulnerability assessment of Wushen Banner in 1960s, 1978, 1988, 2000 and 2008 by using the ecological vulnerability evaluation model established though analytic hierarchy process. The conclusion is that the majority parts of Wushen Banner belong to middle and low vulnerable area. The grassland ecological vulnerability experienced decrease, increase and decrease in recent 50 years, and the grassland ecological vulnerability of southern Wushen Banner is higher than the northern area. Finally, the authors give suggestions about how to protect and utilize the grassland resource based on the evaluation result.