Based on the remains examination of Quaternary glacier in Changbai Mountains and Da Hinggan Mountains, Quaternary glaciers were investigated by the methods of the 3S technologies with geographic information science (GIS), remote sensing (RS) and global positioning system (GPS), drilling and mapping, pollen analysis, fineness and osmotic permeability analysis and 14C dating. The environmental evolution model of Quaternary glacier was reconstructed: the climate was cold and dry in the early stage of early Pleistocene. It was worm and wet in the middle of Early Pleistocene. It was cold and wet in the early of middle Pleistocene; it was cold and a bit wet in the early of Late Pleistocene, worm in the middle, and cold and dry in the late.