The tectonic evolution, petroleum accumulation history, accumulation type and main controlling factors in Huangqiao region of the Lower Yangtze Basin were studied with the balanced cross section, fluid inclusion, geochemical and physical analysis methods. During the late Himalayan, the reservoirs in the Upper Paleozoic were saved and finally formed. The reservoirs found in well HT3 belong to Longtan Formation. They were self-generated and self-reserved. Structures controlled petroleum accumulation. Physical properties influenced reservoir distribution.