The Taipingdong-Zimudang gold deposit, which is hosted in the western Huijiabao anticline, is a major gold deposit in the Huijiabao gold ore-field in the southwest of oft-com:office:smarttags" />Guizhou Province, China. Based on the study of stable isotope geochemistry of the realgar and calcite and REE of calcite, the ore source was discussed. The results show that δ34S values of realgar range from 0.81‰ to 3.03‰ and close to the value of mantle with the range of 2.22‰, mean value of 1.6‰, indistinctive variation and high homogenization, indicating that the ore-forming materials were derived mainly from deep place. During its migration, the calcite of the late stage precipitates as a result of change in temperature, redox condition, etc.