In order to remediate oil contaminated groundwater in loess region of north Shaanxi Province, microbial techniques of optimized native microbial communities were used for experimental studies on oil polluted groundwater remediation. The results of the experiment showed that the oil, with contents of 182.5, 862.5 and 1 695.0 mg/L, added artificially into soil can be reduced by 27.47% to 92.46% after enhanced microbial remediation for 28 to 37 days, whereas the oil degradation rate in non-bacterium reference sample was less than 5%, which suggests that the oil degradation in groundwater with non-bacterium was slow.