Gaseous alkanes from the Xujiaweizi fault depression in the Songliao Basin are characterized by heavier stable carbon isotopes, mostly showing a inverse order or a reverse order. In this paper, a series of experiments concerning the generation and synthesis of gaseous alkanes have been conducted. Mantle-derived carbon dioxide sampled from the Xujiaweizi fault depression was used to synthesize hydrocarbon gas through the Fishcher-Tropsch reaction, and the produced gaseous alkanes yielded a normal stable carbon isotope trend. The results showed that the mixed biogenic end-member gases could also lead to an inverse stable carbon isotopic order, whereas the mixed abiogenic gases are not necessarily reversal. The results suggested that natural gases from the Xujiaweizi fault depression are mainly biogenic.