Huaqing area, located in central Ordos Basin, is one of the main areas of ultra-low permeability reservoirs to be explored and developed. In this study, the authors collected the samples of crude oil produced from the Chang 8, 9, 10 Formations and source rocks in Chang 7, 8 in this area and measured the crude oil and extractable organic matter in source rocks. The authors used the distribution and constitutes of biomarkers of the crude oil and extractable organic matter in source rocks to determine the possible oil source. The results showed that crude oil in the Chang 8, 9, 10 Formations have the same source of Chang 7 Formation.