Water samples collected from different positions of two subterranean rivers in Liuzhou Karst Area are tested for 18 kinds of organochlorine pesticides (OCPs) by gas chromatography coupled with an electron capture detect (GC/ECD). The results show that except dieldrin aldehyde, 17 kinds of OCPs are detected in different levels and the OCPs concentration range from 107.68 to 1 101.55 ng/L, with a mean value 532.515 ng/L. Main OCPs are α-HCH, β-HCH, γ-HCH, δ-HCH, p, p’-DDE, o, p’-DDT, p, p’-DDD, p, p’-DDT, HCB, Heptachlor, Aldrin. Content of the DDTs is the highest. HCHs and OCPs account for 75.8%~96% of the total. Meanwhile, the origins of organchlorinated pesticides in the water are investigated. DDTs mainly comes from soils.