Nanoscale zero-valent iron (NZVI) with 20~40 nm size and Brunauer Emmett Teller (BET) surface area of 49.16 m2/g was synthesized in the laboratory for removal of nitrate from water. Batch experiments were conducted to investigate the denitrification kinetics of NZVI and the factors affecting the denitrification of nitrate. Experimental results suggest that the low pH value is more favorable for nitrate reduction. Additional, in certain limits the reduction rate of nitrate increased with increase of NZVI dosage and decreased with increase of initial concentration of nitrate. The dynamics equation for the pseudo first order reaction was obeyed.