///This paper tries to discuss the mineralizing fluid property during mineralization and simulate metallogenetic mechanism and process quantitatively on basis of characteristics of Fe, Mn, Sr content and C, O, Sr isotope value of calcite from polymetallic deposit. The analysis indicate that 1) the intensity of cathodoluminescence could be used to distinguish calcite of different stages and the content of Fe and Mn increase obviously in calcite during the metalizing phase and the majority content of Mn was more than one percent that is the reflection of post-magmatic hydrothermal activity; 2) the precipitation mechanism of calcite could been distinguished qualitatively based on the conjuncture variational tendency of δ¹³C and δ¹⁸O value and combined with the δ¹³C and δ¹⁸O value in fluid precipitation process of calcite and polymetallic minerals associated with the calcite could been simulated quantitatively; 3) each metallogenic provinces have specific ⁸⁷Sr/⁸⁶Sr value range which is the basis for distinguishing the end fluid; and 4) the radioactive Sr is involved with the mineralization and ore materials are derived from the paleo-aluminosilicate rocks.