Phase fractionation is an important form in the process of adjustment and reconstruction of reservoir for marine carbonate rock measures. Diamantane compound is a kind of hydrocarbon compound with a strong anti-thermolysis and antibiodegradation capability. Through the analysis of diamantane series of crude oil in the typical phase fractionation action area(Lunnan uplift area) of gas washing in Tarim Basin, it is believed that the crude oil after gas washing is characterized by 1-methyladamantane enrichment and depletion of 1, 3-dimethyladamantane, 1, 4-dimethyladamantane, and 3-methyl-1-ethyladamantane. Therefore, the diamantane compounds can be used as an effective tracking mark of phase fractionation.