Daqiaowu deposit is one of the important uranium deposits located in the Ganzhou-Hangzhou metallogenetic belt of volcanogenic uranium deposits. Based on studying of the lead, hydrogen and oxygen isotopes and predecessors data of the deposits, this paper discussed the origin of the ore-forming fluid and source of ore-forming materials of the deposits. The Pb isotopic compositions of pyrites from granite porphyry, tuff, diabase, ore and altered wall rocks show a slight variation, implying that the Pb isotopes may have analogous origins. The hydrogen and oxygen isotope compositions show that the ore-forming fluid is originated from the meteoric water. The ore-forming materials are mainly derived from the crust-mantle mixed source.