MARGIN: 0cm 0cm 0pt" class=MsoNormal>Using the methods and theories of basin analysis and petroleum geology, the structural evolution and petroleum geology feature of mas-microsoft-com:office:smarttags" />Upper Magdalena Basin in Colombia are comprehensively studied. The results show that the basin evolution experiences the four phases. Main source rocks are Tetuan and La Luna limestones which are deposited in passive margin of Cenomanian-Santonian period. The major reservoir rocks are delta facies sandstone of Caballos Formation in Albian period and river-delta facies sandstone of Monserrate Formation Maastrichtian period, and the main regional cap rocks are Villeta Group shale deposited in passive margin and the mudstones of Guaduala Formation deposited in foreland area in Paleocene. Miocene is the key period for oil and gas migration and accumulation. mas-microsoft-com:office:office" />