The Biogenic silica (BSi) contents were determined in the sediments from Arrow Bamboo Lake in Jiuzhaigou National Reserve. Furthermore, the relationship between sediment total organic carbon (TOC) content, grain-size fraction and BSi content were discussed in order to trace the anthropogenic and/or natural effect on water quality and lacustrine sedimentation. The content of BSi ranged between 5.5 and 15.8 mg/g, and the BSi content in the sediments had a significantly positive correlation with TOC. Also, the BSi content was mainly controlled by the grain-size distribution in the core sediments, and the stronger adsorption to BSi occurred in the finer grain fraction.