The data from mineralogy, petrology, isotopic geochemistry, inclusion thermometry and well logging are used to investigate distribution, origin and effect on reservoir properties of calcite cements in overpressure sandstones of Paleogene Anjihaihe Formation on the south margin of Junggar Basin. Due to the inhibiting effect of overpressure on diagenesis, the sandstone cements in the overpressure zone of Anjihaihe Formation are mostly ferrocalcite cements, while the ferrodolomite and quartz overgrowth are seldom. The ferrous calcite cements formed at the late diagenetic stage. In addition, the depth interval with negative migration of δ13CPDB is related to the secondary pore zone formed by organic acid corrosion.