Early Lower Triassic plants are little known in China, especially in South China. This paper presents the paleofloral assemblage from the top of Xuanwei and the lower of the Kayitou Formations in western Guizhou and eastern Yunnan, China.Fossil plants in this area (Annularia shirakii, Lobatannularia sp., Paracalamites stenocostatus, Gigantopteris sp., Pecopteris sp., which are all the Permian relicts) from the bottom of the Kayitou Formation described by other people in 1970s are covered. These fossil plants are in association with an early Lower Triassic marine fauna (Bivalves, Ostracods, Brachiopods and Ammonites). Based on analyzing paleoflora and limnology of the top of Xuanwei and the lower of Kayitou Formations, it is concluded that tropical and humid conditions persisted in western Guizhou and eastern Yunnan throughout the Permian and Earliest Triassic.