The Shuiyindong deposit is a super-large gold deposit in southwestern Guizhou Province, which is famous for its high grade and super large-scale. The geochemical study of isotopes such as S, Pb, C and O shows that the ore-forming material may come from juvenile fluids and crustal rocks. Some isotopic data from other carlin deposits in Yunnan-Guizhou-Guangxi area indicate that the mineralization of gold deposits in this area may closely relate to the evolution of juvenile fluids and some rocks that may be concealed under this area.