Based on the field survey and experiment, the characteristics of hydrogen and oxygen isotopes and hydrochemistry for different waters in Hetao Plain were analyzed by the means of environmental isotope and hydrochemistry technology, the effects of evaporation concentration and human activity on different waters were discussed, and groundwater recharge sources were traced by hydrogen and oxygen isotopes. The results show that slope of meteoric water line in Hetao Plain is less than that of global meteoric water line, and deuterium excess parameters of different waters are less than that of meteoric water line in Baotou area and global meteoric water line, so that water evaporation results in the enrichment of hydrogen and oxygen isotopes; changes of hydrogen and oxygen isotopic compositions of river water, lake water and groundwater are significant, and the isotopic compositions are gradually depleted from lake water to river water, and then to groundwater; hydrochemical types are HCOa-Ca for river water, HCO3-Na, HCOa-Ca and C1-Na for groundwater and SO4-Mg and Cl-Na for lake water; correlations between hydrogen and oxygen isotopic compositions of river water and groundwater and mass concentrations of different ions are not significant, but hydrogen and oxygen isotopic compositions are significantly correlated with mass concentrations of K+, Ca2+, Mg2+, Na+, Cl- and SO42- , pH, conductivity and salinity of lake water; Yellow River water recharges the groundwater in the southern and western of Hetao Plain, and rainfall and piedmont flowing water laterally recharge the groundwater in the north, but hydrogen and oxygen isotopic compositions and mineralization of groundwater in the east are higher near Ulansuhai area, because the effect of lake water is probably significant.