Delay times of surface wave for different station pairs in Yunnan region have been calculated by ambient noise method. The results show that the wave velocity structure changes obviously in different times. These changes include: 1) the wave velocity in Chenghai fault increases due to energy accumulation after the Wenchuan MS8.0 earthquake in 2008; 2) Panzhihua region is located on the boundary of velocity rising and dropping before the Panzhihua MS6.1 earthquake in 2008; and 3) wave velocity increases in the hypocentral area before the Yaoan MS6.0 earthquake in 2009, and reduces after the shock.