Located in the inner and outer contact zones between granite porphyry and metamorphic rocks of Xunwuyan Formation, the Yuanlingzhai molybdenum deposit is a large-sized porphyry molybdenum deposit in southern Jiangxi Province. Re-Os isotopic dating of 5 molybdenite samples from the mineralized quartz veins of this ore deposit has yielded a precise isochron age of (161.1±3.9) Ma and a weighted average model age of (162±1) Ma. The intercept of isochron is (0±2) ng/g, and the MSWD is 0.50. The Yuanlingzhai molybdenum deposit was formed in mid-late Middle Jurassic, and its metallogenic age corresponds to the second ore-forming stage (170~150 Ma) in the Late Jurassic in South China, consistent to regional tungsten-tin mineralization ages around 160 Ma. Molybdenum mineralization occurred in the range of 165~130 Ma in South China.