Pollen and age data have been collected in two cores from the Kunming Basin by the project “Exploration of Active Fault and Evaluation of the Danger of the Seism in the KunmingBasin” to analyze the vegetational change and paleoclimate evolution from late Middle-Pleistocene in this area. The results show that sedimentary environment has an important role in the analysis of pollen records and the paleoclimate evolution of the Kunming Basin from late Middle-Pleistocene. Compared with the paleoclimate history of the Qinghai-Tibetan Plateau and the global records, it is indicated that the paleoclimate evolution in the Kunming Basin is in accordance with the paleoclimate in central and western Yunnan Province.