Biotite is one of the important minerals in the pegmatite-type uranium deposits. It not only exists in the pegmatites and granites which have close relationship with uranium mineraliza- tion,but also is closely related with the main uranium mineral in the deposit. Biotite in the gran- ites,alaskites and pegmatites closely related with the Guangshigou pegmatite-type uranium depos- it is selected as the research subject. Based on the mineral chemistry of biotite analyzed by EM- PA,it is showed that all the biotites are characterized by rich in Mg and Mn,deplete in Ca and the volatile constituents F and C1. The biotite in U-bearing biotite pegmatites is characterized by higher content in Mg,Mn and lower in A1,ACNK and oxygen fugacity,compared to other biotite pegmatite. The results show that the alaskites and pgematites are not from the fractional crystalli- zation of the Huichizhi monzonite granite. It is also evidenced that the assimilation between the pegmatites and wall rock not only control the special structure and composition of biotite in U- bearing pegmatites,but also the key factor in forming of pegmatite-type uranium deposit.