This paper reviewed the tubular microfossils in some detail from the Ediacaran Doushantuo Formation in central Guizhou Province and Yangtze Gorges. These fossils include five form genera and six species types. They are Ramitubus increscens, R. decrescens, Crassitubus costatus, Sinocylocyclicus guizhouensis, Quadratitubus orbigoniatus and Yangtzitubus semiteres. Based on new material and more mophological analyses, it is suggested that the living mode of Ramitubus (with dichotomous branching) might have been benthonic colony, indicating a tabulate coral affinity of Ramitubus is reasonable. However, shapes and inner structures of Crassitubus, Sinocylocyclicus and Quadratitubus indicate that they are more similar to filamentous cyanobacteria.