Large scale ductile shear zones with east-west trending have been recognized in the eastern Tianshan-Beishan Paleozoic orogenic belt along the northern margin of Tarim Basin. They constitute main boundaries between different tectonic units, are considered as the result of the collisional or postcollisional orogenic event. Based on field work, combining with micro-macro structural and geochronological data, seven large scale ductile shear zones were determined, and their extension scale, shear deformation characteristics, deformation conditions, formation time were constrained. The formation process of the ductile shear zones and their roles played in the Paleozoic orogeny of the eastern Tianshan-Beishan orogenic belt were discussed.