Hydrolysis of Heparinase I to Synthetic Heparin-Pentasaccharide and Effect on its Anti-factor Xa Activity.
- 出版年：2001
- 作者：Yu Guangli（于广利） Guan Huashi（管华诗） Robert J. Linhardt2 JawedFareed
- 单位1：Institute of marine Drugs and Foods, Ocean University of Qingdao
- 语种：英文
- 作者关键词：heparinase I；synthetic heparin-pentasaccharide (SHP)；anti-factor Xa activity
- 起始页：529
- 总页数：7
- 刊名：青岛海洋大学学报
- 是否内版：否
- 刊频：双月刊
- 创刊时间：1959
- 主办单位：青岛海洋大学
- 主编：文圣常
- 卷：31
- 期：4
- 期刊索取号：P\226.066\110-1
- 核心期刊：中国自然科学
摘要
The hydrolysis of Heparinase I (from Flavobacterium heparinase, EC 4. 2. 2. 7) to syn-thetic heparin-pentasaccharide (SHP) which contained an antithrombin III binding site was exam-ined. The kinetics of this reaction was studied and the products were separated by stronganion ex-change high performance liquid chromatography (SAX-HPLC). The structure of disaccharide andtrisaccharide derived from SHP were confirmed byNMR and MS techniques. The experiment re-suits showed that, heparinase I, which current!yis used as a heparin reversal agent, can also re-verses this synthetic pentasaccharide and make it losing its anti-factor Xa activity.