贵州甘薯地方种质资源ISSR遗传多样性分析
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摘要
甘薯是贵州省重要的旱地作物之一,长期栽培形成了丰富的甘薯种质资源。本研究对收集到的45份贵州甘薯地方品种资源进行ISSR遗传多样性分析。结果表明,45份贵州甘薯地方种质资源的变异系数在0.07~0.81之间,平均为0.4,表明45份贵州甘薯地方种质资源的亲缘关系较近,思南黄皮桔红心和石阡紫皮黄心的最大,为0.81,瓮安白皮白心和铜仁黄皮桔红心的最小,为0.07。通过ISSR分子标记明晰贵州甘薯地方品种间的亲缘关系,以期为贵州甘薯杂交育种提供一定的理论依据。
引文
[1]贵州统计年鉴.贵州人民出版社,2009.
[2]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting bysimple sequence repeat SSR-anchored polymerase chain reac-tion amplification[J].Genomics,1994,20:176-183.
[3]Nagaoka T,Ogihara Y.Applicability of inter-simple sequencerepeat polymorphisms in wheat for use as DNAmarkers in com-parison to RFLP and RAPD markers[J].Theor Appl Genet,1997,94:597-602.
[4]Pradeep R M,Sarla N,Siddiq E A.Inter simple sequence re-peat ISSR polymorphism and its application in plant breeding[J].Euphytica,2002,128:9-17.
[5]孙洪,程静,詹克慧,等.ISSR标记技术及其在作物遗传育种中的应用[J].分子植物育种,2005,3(1):123-127.
[6]Huang J C,Sun M.Genetic diversity and relationship of sweet-potato and its wild relatives in Ipomoea series Batatas(Convol-vulaceae)as revealed by inter-simple sequence repeat(ISSR)and restriction analysis of chloroplast DNA[J].Theor ApplGenet,2000,100:1 050-1 060.
[7]贺学勤,刘庆昌,王玉萍,等.中国甘薯地方品种的遗传多样性分析[J].中国农业科学,2005,38(2):250-257.
[8]李强,刘庆昌,翟红,等.中国甘薯主要亲本遗传多样性的ISSR分析[J].作物学报,2008,34(6):972-977.
[9]李强,揭琴,刘庆昌,等.甘薯基因组DNA高效快速提取方法[J].分子植物育种,2007,5(5):743-746.
[10]宋吉轩,雷尊国,黄团,等.甘薯ISSR-PCR反应体系的优化[J].贵州农业科学,2009,37(11):25-26,29.
[2]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting bysimple sequence repeat SSR-anchored polymerase chain reac-tion amplification[J].Genomics,1994,20:176-183.
[3]Nagaoka T,Ogihara Y.Applicability of inter-simple sequencerepeat polymorphisms in wheat for use as DNAmarkers in com-parison to RFLP and RAPD markers[J].Theor Appl Genet,1997,94:597-602.
[4]Pradeep R M,Sarla N,Siddiq E A.Inter simple sequence re-peat ISSR polymorphism and its application in plant breeding[J].Euphytica,2002,128:9-17.
[5]孙洪,程静,詹克慧,等.ISSR标记技术及其在作物遗传育种中的应用[J].分子植物育种,2005,3(1):123-127.
[6]Huang J C,Sun M.Genetic diversity and relationship of sweet-potato and its wild relatives in Ipomoea series Batatas(Convol-vulaceae)as revealed by inter-simple sequence repeat(ISSR)and restriction analysis of chloroplast DNA[J].Theor ApplGenet,2000,100:1 050-1 060.
[7]贺学勤,刘庆昌,王玉萍,等.中国甘薯地方品种的遗传多样性分析[J].中国农业科学,2005,38(2):250-257.
[8]李强,刘庆昌,翟红,等.中国甘薯主要亲本遗传多样性的ISSR分析[J].作物学报,2008,34(6):972-977.
[9]李强,揭琴,刘庆昌,等.甘薯基因组DNA高效快速提取方法[J].分子植物育种,2007,5(5):743-746.
[10]宋吉轩,雷尊国,黄团,等.甘薯ISSR-PCR反应体系的优化[J].贵州农业科学,2009,37(11):25-26,29.
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