分离自安徽南部竹黄相关真菌的分子鉴定及多样性分析
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摘要
为鉴定竹黄相关真菌,该试验从安徽南部竹林采集的竹黄菌子实体上分离纯化出50株真菌,对形态上有显著差异的代表性菌株进行18S rDNA/ITS rDNA序列测定,系统学分析表明,这些真菌中48株属于子囊菌门成员,2株为接合菌门成员。在子囊菌中又以肉座菌目和梨孢假壳科成员为优势菌群,其次为隔孢腔菌目、假毛球壳目和炭角菌目。
In order to analyze the fungi associated with ascomata of Shiraia bambusicola collected in southern Anhui Province of eastern China,fungal strains were isolated from ascomata of S. bambusicola. Fifty strains with significant morphological differences were characterized by 18S rDNA and ITS rDNA sequence analyses. Phylogenetic analyses show that two strains belong to the Zygomycota,and the other 48 fungal isolates belong to the Ascomycota,in which the members of the Hypocreales and Apiosporaceae are dominant groups,secondly including the Pleosporales,Trichosphaeriales and Xylariales.
In order to analyze the fungi associated with ascomata of Shiraia bambusicola collected in southern Anhui Province of eastern China,fungal strains were isolated from ascomata of S. bambusicola. Fifty strains with significant morphological differences were characterized by 18S rDNA and ITS rDNA sequence analyses. Phylogenetic analyses show that two strains belong to the Zygomycota,and the other 48 fungal isolates belong to the Ascomycota,in which the members of the Hypocreales and Apiosporaceae are dominant groups,secondly including the Pleosporales,Trichosphaeriales and Xylariales.
引文
[1]贾小明,徐晓红,庄百川,等.药用竹黄菌的生物学研究进展[J].微生物学通报,2006,33(3):147-150.
[2]赖广辉,傅乐意.竹黄主要寄主植物的研究[J].中国野生植物资源,2000,19(1):8-11.
[3]邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京:中国林业出版社,1983.
[4]袁嗣令.中国乔、灌木病害[M].北京:科学出版社,1997.
[5]陈远腾,万象义.竹黄主要光敏有效成分的初步探讨[J].云南大学学报,1981,3(2):104-107.
[6]万象义,陈远腾.一种新的光化学疗法药物———竹红菌甲素[J].科学通报,1980(24):1148-1149.
[7]王景祥,张黎明,朱丽青,等.竹黄化学成分的研究[J].中草药,1990,21(7):4-5.
[8]KISHI T,TAHARA S,TANIGUCHI N,et al.New perylenequinones fromShiraia bambusicola[J].Planta Med,1991,57(4):376-379.
[9]WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNAgenes for phylogenetics[M]//INNIS MA,GELFAND D H,SNINSKYJ J,et al.PCRprotocols:Aguide to methods and applications.New York:Academic Press,1990:315-322.
[10]赵桂华,蒋新瑞,闵祥举,等.日本矮生沿阶草上的真菌分离与鉴定[J].林业实用技术,2008(3):26-27.
[11]付惠,陈玉惠,王文久,等.云南五种用材竹的致病菌及其致霉特性研究[J].竹子研究汇刊,1999,18(1):16-22.
[12]AOKI T,O DONNELL K.Fusariumkyushuensesp.nov.fromJapan[J].Mycoscience,1998,39:1-6.
[13]纪元,毕建男,严冰,等.产紫杉醇真菌的研究概况与紫杉醇工业生产的一个新思路[J].生物工程学报,2006,22(1):1-6.
[14]夏黎明,张素轩,黄建河.毛竹基腐病菌(Arthrinium phaeospermum)的研究[J].南京林业大学学报(自然科学版),1995,19(2):23-28.
[15]LIUX,LUOX,HU W.Studies onthe epidemiology and etiology of moldy sugarcane poisoningin China[J].Biomed Environ Sci,1992,5:161-177.
[16]郑凌凌,朱天辉.枯斑拟盘多毛孢的培养条件对其产毒的影响[J].北京林业大学学报,2006,28(3):115-118.
[17]JAMES K H,ATTA MA,EUGENEJ F,et al.Pestacin:A1,3-dihydroisobenzofuran fromPestalotiopsis microsporapossessing antioxidant and antimycotic activities[J].Tetrahedron,2003,59:2471-2476.
[18]刘家熙.麦角菌[J].生物学通报,1997,32(4):17-18.
[2]赖广辉,傅乐意.竹黄主要寄主植物的研究[J].中国野生植物资源,2000,19(1):8-11.
[3]邵力平,沈瑞祥,张素轩,等.真菌分类学[M].北京:中国林业出版社,1983.
[4]袁嗣令.中国乔、灌木病害[M].北京:科学出版社,1997.
[5]陈远腾,万象义.竹黄主要光敏有效成分的初步探讨[J].云南大学学报,1981,3(2):104-107.
[6]万象义,陈远腾.一种新的光化学疗法药物———竹红菌甲素[J].科学通报,1980(24):1148-1149.
[7]王景祥,张黎明,朱丽青,等.竹黄化学成分的研究[J].中草药,1990,21(7):4-5.
[8]KISHI T,TAHARA S,TANIGUCHI N,et al.New perylenequinones fromShiraia bambusicola[J].Planta Med,1991,57(4):376-379.
[9]WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNAgenes for phylogenetics[M]//INNIS MA,GELFAND D H,SNINSKYJ J,et al.PCRprotocols:Aguide to methods and applications.New York:Academic Press,1990:315-322.
[10]赵桂华,蒋新瑞,闵祥举,等.日本矮生沿阶草上的真菌分离与鉴定[J].林业实用技术,2008(3):26-27.
[11]付惠,陈玉惠,王文久,等.云南五种用材竹的致病菌及其致霉特性研究[J].竹子研究汇刊,1999,18(1):16-22.
[12]AOKI T,O DONNELL K.Fusariumkyushuensesp.nov.fromJapan[J].Mycoscience,1998,39:1-6.
[13]纪元,毕建男,严冰,等.产紫杉醇真菌的研究概况与紫杉醇工业生产的一个新思路[J].生物工程学报,2006,22(1):1-6.
[14]夏黎明,张素轩,黄建河.毛竹基腐病菌(Arthrinium phaeospermum)的研究[J].南京林业大学学报(自然科学版),1995,19(2):23-28.
[15]LIUX,LUOX,HU W.Studies onthe epidemiology and etiology of moldy sugarcane poisoningin China[J].Biomed Environ Sci,1992,5:161-177.
[16]郑凌凌,朱天辉.枯斑拟盘多毛孢的培养条件对其产毒的影响[J].北京林业大学学报,2006,28(3):115-118.
[17]JAMES K H,ATTA MA,EUGENEJ F,et al.Pestacin:A1,3-dihydroisobenzofuran fromPestalotiopsis microsporapossessing antioxidant and antimycotic activities[J].Tetrahedron,2003,59:2471-2476.
[18]刘家熙.麦角菌[J].生物学通报,1997,32(4):17-18.
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