贵州地道药材半夏的HPLC指纹图谱研究
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摘要
为建立贵州地道药材半夏的指纹图谱研究方法,为半夏的质量控制提供依据,采用HPLC法对11批贵州不同产地的半夏药材进行指纹图谱研究。结果表明,测定的11批半夏样品有18个共有峰,共有峰峰面积的RSD在6.84%~38.37%,相对峰面积的RSD在0.02%~38.63%;共有峰的相对保留时间从0.196~1.710,相对保留时间的RSD在0.02%~1.93%,该方法具有良好的稳定性和重复性。建立的贵州地道药材半夏的HPLC指纹图谱分析方法,较为全面地反映了半夏中化学成分的信息,为贵州地道药材半夏的质量控制提供了科学试验依据。
The fingerprint chromatograph of 11 Pinellia ternata samples collected from different cultivation fields were analyzed by HPLC to establish the analysis method of Pinellia ternata fingerprint chromatograph.The results showed that there were 18 common peaks in 11 samples,the RSD of the common peaks area and relative peak area was 6.84%~38.37% and 0.02%~38.63% respectively,the relative retention time of the common peaks was between 0.196 min and 1.710 min,and the RSD of the relative retention time was 0.02%~1.93%,which indicates the analysis method with good stability and repeatability can completely reflect chemical components information to provide the technological basis for quality control of Pinellia ternata in Guizhou.
The fingerprint chromatograph of 11 Pinellia ternata samples collected from different cultivation fields were analyzed by HPLC to establish the analysis method of Pinellia ternata fingerprint chromatograph.The results showed that there were 18 common peaks in 11 samples,the RSD of the common peaks area and relative peak area was 6.84%~38.37% and 0.02%~38.63% respectively,the relative retention time of the common peaks was between 0.196 min and 1.710 min,and the RSD of the relative retention time was 0.02%~1.93%,which indicates the analysis method with good stability and repeatability can completely reflect chemical components information to provide the technological basis for quality control of Pinellia ternata in Guizhou.
引文
[1]国家药典委员会.中国药典(一部)[M].北京:化学工业出版社,2005:78-79.
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[10]李西文,马小军.半夏不同生长发育时期总生物碱含量动态变化的研究[J].中国中药杂志,2006,31(8):687-688.
[11]任德权.中药指纹图谱质控技术的意义与作用[J].新药与临床药理,2001,12(3):135-137.
[2]张科卫,吴皓,崔小兵.不同产区半夏药材中鸟苷含量的测定[J].中成药,2000,22(11):769-771.
[3]杨玉琴,张丽艳.高效液相色谱法测定不同半夏炮制品中麻黄碱的含量[J].贵州医药杂志,2001,25(9):846-847.
[4]张科卫,吴皓.半夏药材中脂肪酸成分的研究[J].南京中医药大学学报,2002,18(5):291-292.
[5]于超,张明.紫外分光光度法测定不同产地半夏中总生物碱的含量[J].时珍国医国药,2002,13(2):73-75.
[6]石国明,肖东华.HPLC测定复方半夏片中麻黄碱的含量[J].中成药,2003,25(10):附4.
[7]许腊英.半夏化学成分及饮片的现代研究进展[J].时珍国医国药,2004,15(7):441-443.
[8]曾建红,彭正松.半夏总生物碱含量的动态变化[J].中药材,2004,27(7):471-473.
[9]于超,张明.栽培、野生及不同产地半夏总生物碱测定[J].中国中药杂志,2004,29(6):583-584.
[10]李西文,马小军.半夏不同生长发育时期总生物碱含量动态变化的研究[J].中国中药杂志,2006,31(8):687-688.
[11]任德权.中药指纹图谱质控技术的意义与作用[J].新药与临床药理,2001,12(3):135-137.
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