云南鼠疫菌Asc I酶切分型及其流行病学意义
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摘要
目的构建以Asc I酶切的云南省鼠疫菌PFGE图谱库并探究其流行病学意义。方法采用限制性内切酶Asc I对云南家、野鼠型及玉龙鼠疫菌进行酶切分析,并对电泳图谱进行聚类分析。结果 30株被试菌株分为15种PFGE型别(相似性系数为88.9%~100.0%)、5个簇、4个群,除404号菌与EV76归为一群,其余3个群分别为家鼠型基因群、野鼠型基因群及玉龙基因群。结论云南鼠疫菌PFGE基因型与生态型相吻合,具有一定的区域聚集性;玉龙鼠疫菌是云南独立的一个基因群。
Objective To create fingerprint library of Yunnan Yersinia pestis by pulse field gel electrophoresis(PFGE) with Asc I enzyme digestion and to study its epidemiological significance. Methods We used rare cutting restriction enzyme Asc I to digest Yunnan Y. pestis strains isolated from Rattus flavipectus plague focus, Apodemus chevrieri-Eothenomys miletus plague focus and Yulong plague focus, and submitted fingerprints to Bionumerics software for cluster analysis. Results Thirty tested strains were divided into 15 genotypes with 88.9%-100.0% similarity value. 15 genotypes formed 4 groups of 5 clusters, one cluster consisted of No.404 and EV76, the other three were of Rattus flavipectus plague group, Apodemus chevrieri-Eothenomys miletus plague group and Yulong group, respectively. Conclusions PFGE genotypes of Yunnan Y. pestis accord with their ecotypes, and have cluster regional feature. The strains isolated from Yulong, are of a unique PFGE type, indicating a new clone group.
Objective To create fingerprint library of Yunnan Yersinia pestis by pulse field gel electrophoresis(PFGE) with Asc I enzyme digestion and to study its epidemiological significance. Methods We used rare cutting restriction enzyme Asc I to digest Yunnan Y. pestis strains isolated from Rattus flavipectus plague focus, Apodemus chevrieri-Eothenomys miletus plague focus and Yulong plague focus, and submitted fingerprints to Bionumerics software for cluster analysis. Results Thirty tested strains were divided into 15 genotypes with 88.9%-100.0% similarity value. 15 genotypes formed 4 groups of 5 clusters, one cluster consisted of No.404 and EV76, the other three were of Rattus flavipectus plague group, Apodemus chevrieri-Eothenomys miletus plague group and Yulong group, respectively. Conclusions PFGE genotypes of Yunnan Y. pestis accord with their ecotypes, and have cluster regional feature. The strains isolated from Yulong, are of a unique PFGE type, indicating a new clone group.
引文
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[6]赵海红,戴二黑,周冬生,等.云南、福建和贵州省鼠疫耶尔森菌基因分型研究[J].中华微生物学和免疫学杂志,2005,25(11):878-879.
[7]史献明,海荣,俞东征,等.中国鼠疫菌随机引物扩增多态性指纹图谱[J].中国地方病学杂志,2003,22(6):548-551.
[8]Zhang XA,Hai R,Wei JC,et al.MLVA distribution characteristics of Yersinia pestis in China and the correlation analy sis[J].BMC Microbiology,2009,23(9):205.
[9]Tenover FC,Arbeit RD,Goering RV,et al.Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:Criteria for bacterial strain typing[J].J Clin Microbio,1995,33(9):2233-2239.
[2]郭英,张丽云,夏连续,等.云南省玉龙县鼠疫菌的生化特性[J].地方病通报,2008,23(3):12-14.
[3]Zhang ZK,Hai R,Song ZZ,et al.Spatial variation of Yersinia pestis from Yunnan Province of China[J].Am J Trop Med Hyg,2009,81(4):714-717.
[4]王鹏,郭英,石丽媛,等.云南省家、野两型鼠疫菌生化特性及营养需求的比较研究[J].中国地方病学杂志,2003,22(3):217-220.
[5]吴明寿,戴二黑,郭英,等.云南鼠疫耶尔森菌基因分型研究[J].中国地方病学杂志,2005,24(5):475-478.
[6]赵海红,戴二黑,周冬生,等.云南、福建和贵州省鼠疫耶尔森菌基因分型研究[J].中华微生物学和免疫学杂志,2005,25(11):878-879.
[7]史献明,海荣,俞东征,等.中国鼠疫菌随机引物扩增多态性指纹图谱[J].中国地方病学杂志,2003,22(6):548-551.
[8]Zhang XA,Hai R,Wei JC,et al.MLVA distribution characteristics of Yersinia pestis in China and the correlation analy sis[J].BMC Microbiology,2009,23(9):205.
[9]Tenover FC,Arbeit RD,Goering RV,et al.Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:Criteria for bacterial strain typing[J].J Clin Microbio,1995,33(9):2233-2239.
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