利用分子标记辅助选择提高江苏粳稻稻瘟病抗性
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摘要
以江苏主栽粳稻品种徐稻3号为轮回亲本,通过分子标记辅助选择,分别构建携带Pi1、Pi2单基因和聚合双基因的近等基因系。人工接种结果表明:与轮回亲本接种结果相比,在携带Pi1的情况下,苗瘟抗性频率提高近30%,穗瘟抗性表现为中感或感的水平;在携带Pi2的情况下,苗瘟抗性频率提高40%以上,穗瘟抗性表现为中抗/中感水平;而聚合Pi1/Pi2的情况下,苗瘟抗性频率提高50%以上,穗瘟抗性达到高抗水平;苗瘟抗性和穗瘟抗性表现明显相关性。
In this study,the donors of resistant genes,Pil and Pi2,were backcrossed with Xudao 3,one of main cultivars grown in Jiangsu province.The near isogenic lines(NILs)with both Pi1 and Pi2 genes and with either of them were successfully developed through molecular marker-assisted selection(MAS).The results showed that the resistance frequency of NIL carrying Pi1 was improved 30%compared with the resistance frequency of Xudao 3 at seedling stage by artificial inoculation and the panicle blast resistance was subjected to medium susceptible or susceptible.Similarly,the resistance frequency of NIL carrying Pi2 was improved by more than 40%at seedling stage by artificial inoculation and the panicle blast resistance was subjected to medium resistant or medium susceptible.The resistance frequency of pyramiding line with Pi1/Pi2 exceeded 50%over the resistance frequency of Xudao 3 and the panicle blast resistance reached high resistance level.The seedling blast resistance was significantly correlated with the panicle blast resistance.
In this study,the donors of resistant genes,Pil and Pi2,were backcrossed with Xudao 3,one of main cultivars grown in Jiangsu province.The near isogenic lines(NILs)with both Pi1 and Pi2 genes and with either of them were successfully developed through molecular marker-assisted selection(MAS).The results showed that the resistance frequency of NIL carrying Pi1 was improved 30%compared with the resistance frequency of Xudao 3 at seedling stage by artificial inoculation and the panicle blast resistance was subjected to medium susceptible or susceptible.Similarly,the resistance frequency of NIL carrying Pi2 was improved by more than 40%at seedling stage by artificial inoculation and the panicle blast resistance was subjected to medium resistant or medium susceptible.The resistance frequency of pyramiding line with Pi1/Pi2 exceeded 50%over the resistance frequency of Xudao 3 and the panicle blast resistance reached high resistance level.The seedling blast resistance was significantly correlated with the panicle blast resistance.
引文
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[13]周益军,范永坚,吴淑华,等.稻瘟病菌生理小种离体兼职鉴定和致病性人工诱变研究[J].扬州大学学报:农业与生命科学版,1999,20(1):34—38.
[14]罗楚平,倪磊,陈志谊,等.水稻稻瘟病接种技术及2009年江苏省区试品种抗性鉴定[J].江苏农业科学,2009(6):178-179.
[15]杨杰,杨金欢,王军,等.稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布[J].华北农学报,2011,26(3):1-6.
[16]刘永峰,陈志谊,刘邮洲,等.2001~2010年江苏省稻瘟病菌种群变化分析[J].江苏农业学报,2010,26(6):1233-1237.
[17]Hittalmani S,Parco A,Mew T W.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theor Appl Genet,2000,100(7):1121-1128.
[183何月秋,唐文华,Leung H.C039近等基因系抗稻瘟病性分析[J].作物学报,200I,27(6):838-841.
[19]施德,孙漱沅.水稻品种对叶瘟和穗瘟的抗性反应及遗传初探[J].浙江农业学报,1989,1(2):94-96.
[20]彭洪江,彭仕钟,吴先丽.水稻稻瘟病叶瘟与穗颈瘟相关性分析[J].西南农业学报,1995,8(4):79-83.
[21]李华,顾才东.叶瘟与穗颈瘟相关性分析[J].宁夏农业科技,1 996(2):21-22.
[22]张磊,张启军,程兆榜,等.4套水稻稻瘟病鉴别品种对江苏地区主要稻瘟病菌株的抗性分析[J].江苏农业学报,2010,26(5):948-953.
[2]杨勤忠,林菲.冯淑杰,等.水稻稻瘟病抗性基因的分子定位及克隆研究进展[J].中国农业科学,2009,42(5):1601.
[3]Dai L Y,Liu X L,Xiao Y H,et al.Recent advances in cloning and characterization of disease resistance genes in rice[J].Journal of Integrative Plant Biology,2007,49(1):112-119.
[4]吴俊,刘雄伦,戴良英,等[J].水稻广谱抗稻瘟病基因研究进展[J].生命科学,2007,19(2):233-238.
[5]Chen H,Chen B,Zhang D,et al.Pathotypes of pyricularia grisea in rice fields of central and southern China[J].Plant Disease,2001,85(8):843-850.
[6]Chen D,Zeigler R,Ahn S,et al.Phenotypic characterization of the rice blast resistance gene Pi-2(t)[J].Plant Disease,1996,80(1):52-56.
[7]陈红旗,陈宗祥,倪深,等.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学,2008,22(1):23-27.
[8]Liu W G,Wang F,Jin S J,et al.Improvement of rice blast resistance in TGMS line by pyramiding of Pi-1 and Pi-2 through molecular marker-assisted selection[J].Acta Agron Sin,2008,34(7):1128-1136.
[9]Jiang H C,Feng Y T,Bao L,et al.Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding[J].Molecular Breeding,2012,30(4):1679-1688.
[10]Inukai T,Nelson R,Zeigler R,et al.Allelism of blast resistance genes in near-isogenic lines of rice[J].Phytopathology,1994,84(11):1278-1283.
[11]于苗苗,戴正元,潘存红,等.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pil的互作效应[J].作物学报,2013,39(11):1927-934.
[12]Hospital F,Chevalet C,Mulsant P.Using markers in gene introgression breeding programs[J].Genetics,1992,132(4):1199-1210.
[13]周益军,范永坚,吴淑华,等.稻瘟病菌生理小种离体兼职鉴定和致病性人工诱变研究[J].扬州大学学报:农业与生命科学版,1999,20(1):34—38.
[14]罗楚平,倪磊,陈志谊,等.水稻稻瘟病接种技术及2009年江苏省区试品种抗性鉴定[J].江苏农业科学,2009(6):178-179.
[15]杨杰,杨金欢,王军,等.稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布[J].华北农学报,2011,26(3):1-6.
[16]刘永峰,陈志谊,刘邮洲,等.2001~2010年江苏省稻瘟病菌种群变化分析[J].江苏农业学报,2010,26(6):1233-1237.
[17]Hittalmani S,Parco A,Mew T W.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theor Appl Genet,2000,100(7):1121-1128.
[183何月秋,唐文华,Leung H.C039近等基因系抗稻瘟病性分析[J].作物学报,200I,27(6):838-841.
[19]施德,孙漱沅.水稻品种对叶瘟和穗瘟的抗性反应及遗传初探[J].浙江农业学报,1989,1(2):94-96.
[20]彭洪江,彭仕钟,吴先丽.水稻稻瘟病叶瘟与穗颈瘟相关性分析[J].西南农业学报,1995,8(4):79-83.
[21]李华,顾才东.叶瘟与穗颈瘟相关性分析[J].宁夏农业科技,1 996(2):21-22.
[22]张磊,张启军,程兆榜,等.4套水稻稻瘟病鉴别品种对江苏地区主要稻瘟病菌株的抗性分析[J].江苏农业学报,2010,26(5):948-953.
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