上海市人类免疫缺陷病毒阳性人群HIV-1分布特征分析
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摘要
[目的 ] 分析上海市人类免疫缺陷病毒 (HIV )阳性人群HIV -1分布特征 ,为制定预防HIV传播措施提供科学依据。 [方法 ] 选择 40例不同感染途径的HIV阳性者 ,提取其血浆中核酸进行扩增 ,然后进行序列测定 ,确定其HIV -1型别。 [结果 ] 3 8例分离到核酸的阳性者中 ,发现 4种HIV -1亚型 (A、B、B’和C)、2种重组亚型 (CRF0 1_AE和CRF0 8_BC)和 1种未见报道的亚型 (CRF0 1_AEenvBgagUpol)。不同人群感染毒株不同 ,经性接触传播感染者感染毒株较为广泛 ( 7种 ) ,而经血传播者都为B/B’亚型。 [结论 ] 上海地区存在着多样的HIV -1亚型和重组毒株
Objective To investigate the genetic diversity of Human Immunodeficiency Virus type I(HIV-1) circulating in Shanghai and to provide scientific evidence for prevention of transmission. [Methods] 40 HIV infections were selected as study subjects. HIV-1 env, gag, pol genes in infected plasma samples were amplified by RT-PCR, sequenced and phylogenetically analyzed. [Results] Four HIV-1 subtype (A, B, B' and C), two circulating recombinant forms (CRFs)(CRF01_AE and CRF08_BC) and one new intersubtype recombinant CRF_01ABU were identified. There were different strains in different target groups. The target group infected through sexual contact had broad strains (7 subtypes) while the target group infected through blood contact only had the same B/B' strains. [Conclusion] The broad HIV-1 diversity exists in Shanghai.
Objective To investigate the genetic diversity of Human Immunodeficiency Virus type I(HIV-1) circulating in Shanghai and to provide scientific evidence for prevention of transmission. [Methods] 40 HIV infections were selected as study subjects. HIV-1 env, gag, pol genes in infected plasma samples were amplified by RT-PCR, sequenced and phylogenetically analyzed. [Results] Four HIV-1 subtype (A, B, B' and C), two circulating recombinant forms (CRFs)(CRF01_AE and CRF08_BC) and one new intersubtype recombinant CRF_01ABU were identified. There were different strains in different target groups. The target group infected through sexual contact had broad strains (7 subtypes) while the target group infected through blood contact only had the same B/B' strains. [Conclusion] The broad HIV-1 diversity exists in Shanghai.
引文
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[2]ShaoYM ,SuL ,SunXH ,etal.MolecularepidemiologyofHIVinfec tioninChina[C].XIIInternationalConferenceonAIDS .Geneva,June1998,131~132.
[3]ShaoY .AIDSinSouthandSoutheastAsia[M ].HIV/AID :Perspec tiveonChina.AIDSPatientCareandSTD .2001,15:431~432.
[4]BoomR ,SolCJA ,SalimansMMM ,etal.Rapidandsimplemethodforpurificationofnucleicacid[J].JClinMicrobial1990;28:495~503.
[5]DelwartEL ,SphaerEG ,LouwagieJ ,etal.Geneticrelationshipdeter minedbyaDNAheteroduplexmobilityassay:analysisofHIV~1 genes[J].Science1993;262:1257~1261.
[6]HeynddrickxL ,JanssensW ,ZekengL ,etal.Simplifiedstrategyfordetectionofrecombinanthumanimmunodeficiencyvirustype1groupMisolatesbygag/envheteroduplexmobilityassay[J].JVirol.200;74:363~370.
[7]ThompsonJD ,GiblsonTJ,PlewniakF ,etal.TheCLUSTALXwin dowsinterface:Flexiblestrategiesformultiplesequencealignmentaidedbyqualityanalysistools[J].NucleicAcidRes.1997;25:4876~4882.
[8]VandePeerY ,andDeWachterR .Treecomforwindows:asoftwarepackagefortheconstructionanddrawingofevolutionarytreesfortheMicrosoftWindowsenvironment[J].Computer.Appl.Biosci.1994;10:569~570.
[9]SuL ,GrafM ,ZhangY ,etal.Characterizationofavirtuallyfull-lengthhumanimmunodeficiencyvirustype1 genomeofaprevalentin tersubtype(C/B’)recombinantstraininChina[J].JVirol2000;74:11367~11376.
[10]ChengH ,ZhangJ,CapizziJ,etal.HIV -1subtypeEinYunnan,China[J].Lancet1994;344:953~954.
[11]YangR ,XiaX ,KusagawaS ,etal.On-goinggenerationbreakpointsuggestsacommonparentoftwoBCrecombinantHIVtype1strainscirculatinginChina[J].AIDSResHumRetroviruses2002;18:1135~1140.
[12]严延生,翁育伟,陈舸,等.福建省艾滋病感染得流行病学和病毒亚型之间相互关系的研究[J].中华流行病学杂志,2001,22(6):428~431.
[13]冯铁建,陈琳,何建凡,等.不同亚型的人类免疫缺陷病毒I型毒株混合感染分析[J].中华预防医学杂志,2001,35(2):117~120.
[14]雷世光,孙显光,邵一鸣,等.贵州省HIV -1感染毒株膜蛋白基因序列测定及流行病学分析[J].中华皮肤科杂志,2000,33(3):149~151.
[15]韩卫国,苏玲,王哲,等.河南省人免疫缺陷病毒流行株的基因亚型分析[J].中华传染病杂志,1999,17(4):253~255.
[16]陈杰,刘伟,NaacyL ,等.广西HIV -1首次流行的分子流行病学分析[J].中华流行病学杂志,1999,2:74~77.
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