贵州省新发现HIV-1毒株亚型特征研究
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摘要
目的分析研究贵州省HIV-1亚型毒株分布及流行特征,为艾滋病防治及疫苗研制提供科学依据。方法 用套式基因扩增方法(PCR)扩增HIV-1毒株gag基因和env基因并测序分析。结果 128例样本扩增、测序成功119例,其中CRF01-AE亚型62例,CRF07-BC重组亚型49例,CRF08-BC重组亚型2例,B亚型6例。结论 本次研究贵州省HIV-1毒株以新发现的CRF01-AE重组亚型流行为主,CRF07-BC重组亚型次之,多亚型并存;分析结果提示贵州省HIV-1毒株亚型向性传播转变。
Objective To study the HIV-1 subtype strain distribution and epidemic characteristics in Guizhou province and to provide scientific data for HIV/AIDS prevention and control.Methods Using a nested Polymerase Chain Reaction(PCR)to amplify gag and env gene of HIV strains,and for sequencing.Results 119 samples were amplified and sequenced successfully among 128 samples,of which 62 subtype CRF01-AE,49 recombinant subtype CRF07-BC,2 recombinant subtype CRF08-BC and 6 subtype B.Conclusion The study showed that newly discovered recombinant subtype CRF01-AE was most prevalent in Guizhou province.recombinant subtype CRF07-BC was the second one.Different subtypes of HIV stains existed.The results suggest that the subtypes of HIVstains will change to sexual transmission.
Objective To study the HIV-1 subtype strain distribution and epidemic characteristics in Guizhou province and to provide scientific data for HIV/AIDS prevention and control.Methods Using a nested Polymerase Chain Reaction(PCR)to amplify gag and env gene of HIV strains,and for sequencing.Results 119 samples were amplified and sequenced successfully among 128 samples,of which 62 subtype CRF01-AE,49 recombinant subtype CRF07-BC,2 recombinant subtype CRF08-BC and 6 subtype B.Conclusion The study showed that newly discovered recombinant subtype CRF01-AE was most prevalent in Guizhou province.recombinant subtype CRF07-BC was the second one.Different subtypes of HIV stains existed.The results suggest that the subtypes of HIVstains will change to sexual transmission.
引文
[1]Jensen MA,Li FS,van′t Wout AB,et al.I mproved core-ceptor usage prediction and genotypic monitoring of R5-to-X4 transition by motif analysis of human i mmunodefi-ciency virus type 1 env V3 loop sequences[J].J Virol,2003,77(24):13376-13388.
[2]Kandathil AJ,Ramalingam S,Kannangai R,et al.Molecu-lar epidemiology of HIV[J].Indian J Med Res,2005,12(1):333-344.
[3]赵全壁,潘品良,温宁,等.中国艾滋病病毒1型流行株V3环序列变异性研究[J].中国性病艾滋病防治,2002,8(4):208-211.
[4]程春林,冯毅,何翔,等.中国南方四省区流行的HIV-1CRFO1-AE病毒株基因特征研究[J].中华流行病学杂志,2009,7(26):1097-1111.
[5]Zhang Y,Lu L,Ba L,et al.Dominance of HIV-1 subtypeCRF01-AEin sexually acquired cases leads to a new epi-demic in Yunnan province of China[J].PLoS Med,2006,3(11):e443.
[6]雷世光,孙显光,邵一鸣,等.贵州省HIV-1感染毒株膜蛋白基因序列测定及流行病学分析[J].中华皮肤科杂志,2000,33(4):149-151.
[7]孙显光,雷世光.贵州省HIV-1毒株亚型分布研究[J].贵州医药,2007,31(7):582-584.
[8]McCutchan FE,Hegerich PA,Brennan TP,et al.Geneticvariants of HIV-1 in Thailand[J].AIDS Res Hum Retro-viruses,1992,8(11):1887-1895.
[9]Cheng H,Zhang J,Capizzi J,et al.HIV-1 subtype E inYunnan,China[J].Lancet,1994,344(8927):953-954.
[10]Luo CC,Tian C,Hu DJ,et al.HIV-1 subtype Cin China[J].Lancet,1995,345(8956),1051-1052.
[11]邵一鸣,邢辉,严延生,等.福建省HIV-l流行毒株的基因序列测定和亚型分析[J].中华微生物学和免疫学杂志,2000,5(20):458-461.
[12]刘丽花,杨丽华,赵欣荣,等.不同途径感染人群HIV-1亚型分布[J].中国公共卫生,2009,8(25):1023-1024.
[13]秦光明,邵一鸣,刘刚,等.四川省HIV-1流行毒株的墓基因序列测定和亚型分析[J].中华流行病学杂志,1998(1):39-42.
[14]邵一鸣,赵峰,杨维中,等.我国西南西北地区吸毒人群重组人类免疫缺陷病毒1型毒株的发现[J].中国实验和临床病毒学杂志,1999,13(2):109-112.
[15]辛若雷,何翔,邵一鸣.HIV-1 CRF07-BC重组毒株的起源和分子流行病学研究进展[J].中国艾滋病性病,2009,5(2):200-202.
[2]Kandathil AJ,Ramalingam S,Kannangai R,et al.Molecu-lar epidemiology of HIV[J].Indian J Med Res,2005,12(1):333-344.
[3]赵全壁,潘品良,温宁,等.中国艾滋病病毒1型流行株V3环序列变异性研究[J].中国性病艾滋病防治,2002,8(4):208-211.
[4]程春林,冯毅,何翔,等.中国南方四省区流行的HIV-1CRFO1-AE病毒株基因特征研究[J].中华流行病学杂志,2009,7(26):1097-1111.
[5]Zhang Y,Lu L,Ba L,et al.Dominance of HIV-1 subtypeCRF01-AEin sexually acquired cases leads to a new epi-demic in Yunnan province of China[J].PLoS Med,2006,3(11):e443.
[6]雷世光,孙显光,邵一鸣,等.贵州省HIV-1感染毒株膜蛋白基因序列测定及流行病学分析[J].中华皮肤科杂志,2000,33(4):149-151.
[7]孙显光,雷世光.贵州省HIV-1毒株亚型分布研究[J].贵州医药,2007,31(7):582-584.
[8]McCutchan FE,Hegerich PA,Brennan TP,et al.Geneticvariants of HIV-1 in Thailand[J].AIDS Res Hum Retro-viruses,1992,8(11):1887-1895.
[9]Cheng H,Zhang J,Capizzi J,et al.HIV-1 subtype E inYunnan,China[J].Lancet,1994,344(8927):953-954.
[10]Luo CC,Tian C,Hu DJ,et al.HIV-1 subtype Cin China[J].Lancet,1995,345(8956),1051-1052.
[11]邵一鸣,邢辉,严延生,等.福建省HIV-l流行毒株的基因序列测定和亚型分析[J].中华微生物学和免疫学杂志,2000,5(20):458-461.
[12]刘丽花,杨丽华,赵欣荣,等.不同途径感染人群HIV-1亚型分布[J].中国公共卫生,2009,8(25):1023-1024.
[13]秦光明,邵一鸣,刘刚,等.四川省HIV-1流行毒株的墓基因序列测定和亚型分析[J].中华流行病学杂志,1998(1):39-42.
[14]邵一鸣,赵峰,杨维中,等.我国西南西北地区吸毒人群重组人类免疫缺陷病毒1型毒株的发现[J].中国实验和临床病毒学杂志,1999,13(2):109-112.
[15]辛若雷,何翔,邵一鸣.HIV-1 CRF07-BC重组毒株的起源和分子流行病学研究进展[J].中国艾滋病性病,2009,5(2):200-202.
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