贵州地方水稻品种“禾”的SSR指纹图谱构建
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摘要
利用SSR标记对24份"禾"品种进行遗传多样性分析,初步建立了其DNA指纹图谱。从145对SSR引物中筛选出21对多态性引物组合,对所有供试材料进行分析,共检测出73个等位基因,平均为3.4762;平均PIC指数为0.4084,范围在0.1175(RM147)~0.6810(RM336)。应用6对引物(RM20A、RM161、RM253、RM280、RM336和RM495)的组合获得了每个品种的SSR特征带,可以将所有供试材料区分开来,从而建立24份"禾"品种的SSR指纹图谱。UPGMA聚类分析表明,在相似系数为0.652处,可将所有材料分为5类,聚类结果表明品种间的亲缘关系与地理来源关系不大。
The DNA fingerprint map of 24 local rice varieties was established from analyzing the genetic diversity by using SSR marker in Guizhou.73 alleles were detected from tested rice varieties by using 21 pairs of primer combinations with polymorphism screened from 145 pairs of SSR primers.The average number of alleles per locus was 3.4762 and its average PIC index with 0.4084 varied from 0.1175(RM147)-0.6810(RM336).The SSR fingerprint maps of 24 local rice varieties were established according to the SSR characteristic bands identified by using six pairs of primer combinations(RM20A,RM161,RM253,RM280,RM336 and RM495).The UPGMA cluster analysis showed that 24 local rice varieties could be divided into five groups at 0.652 of the similar coefficient.The cluster results showed that there was no closer genetic relationship in blood relationship and geographical source among 24 local rice varieties in Guizhou.
The DNA fingerprint map of 24 local rice varieties was established from analyzing the genetic diversity by using SSR marker in Guizhou.73 alleles were detected from tested rice varieties by using 21 pairs of primer combinations with polymorphism screened from 145 pairs of SSR primers.The average number of alleles per locus was 3.4762 and its average PIC index with 0.4084 varied from 0.1175(RM147)-0.6810(RM336).The SSR fingerprint maps of 24 local rice varieties were established according to the SSR characteristic bands identified by using six pairs of primer combinations(RM20A,RM161,RM253,RM280,RM336 and RM495).The UPGMA cluster analysis showed that 24 local rice varieties could be divided into five groups at 0.652 of the similar coefficient.The cluster results showed that there was no closer genetic relationship in blood relationship and geographical source among 24 local rice varieties in Guizhou.
引文
[1]张再兴.贵州地方稻种资源分布及其与生态环境的关系[J].贵州农业科学,1983(3):1-4.
[2]杨昌润.美丽富饶的黔东南[J].贵州文史丛刊,1994(1):42-43.
[3]程良炳.地方稻种——禾的特点及其利用意见[J].贵州农业科学,1981(2):23-27.
[4]张再兴.贵州稻种资源[A].见:应存山.中国稻种资源[M].北京:中国农业出版社,1993.201-222.
[5]倪克鱼,孙灿慧.中国稀有稻类贵州禾的光亲和性及评价[J].贵州农业科学,2000,28(3):3-5.
[6]阮仁超,杨宗烺,陈龙,等.糯稻良种农虎禾在贵州立体农业生态条件下适应性初探[J].贵州农业科学,1992(5):25-29.
[7]肖小余,王玉平,张建勇,等.四川省主要杂交稻亲本的SSR多态性分析和指纹图谱的构建与应用[J].中国水稻科学,2006,20(1):1-7.
[8]赵波,金文林,濮绍京,等.基于SSR小豆新品种(系)DNA指纹图谱构建及亲缘分类初探[J].北京农学院学报,2008(3):1-6.
[9]于永红,李云海,马荣荣,等.用微卫星DNA标记建立宁2A的指纹图谱[J].中国水稻科学,2001,15(3):215-217.
[10]王笑一,于拴仓,张凤兰,等.小白菜品种的SSR指纹图谱及遗传特异性分析[J].华北农学报,2008,23(5):97-103.
[11]Hamwieh A,Udupa S M,Choumane W,et al.A genetic linkagemap of Lens sp.based on microsatellite and AFLP markers and thelocalization of Fusarium vascular Wilt resistance[J].Theor ApplGenet,2005,110:669-677.
[12]黎裕,贾继增,王天宇.分子标记的种类及其发展[J].生物技术通报,1999,15(4):19-22.
[13]张献龙,唐克轩.植物生物技术[M],北京:科学出版社,2004.401-500.
[14]Dellaporta S L,Wood J,Hicks J B.A plant DNA mini preparation:Version II[J].Plant Mol Biol,1983(1):19-21.
[15]Doyle J J,Doyle J L.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:149-151.
[16]宗绪晓,D Vaughan,A Kaga,等.AFLP分析小豆种(Vigna angu-laris)内遗传多样性[J].作物学报,2003,29(4):562-568.
[17]Liu KJ,Muse S V.PowerMarker:Integrated analysis environment forgenetic marker data[J].Bioinf ormatics,2005,21:2121-2129.
[18]Smith J S C,Chin E C L,Shu H,et al.An evaluation of the utilityof SSR loci as molecular markers in maize(Zea maysL.):Compari-son with data fromRFLPs and pedigree[J].Theor Appl Genet,1997,95:163-173.
[19]Rohlf F J.NTSYS-pc:Numerical taxonomy and multivariate analysissystem,version 2.1.Exeter Publications[M].New York:Exeter Pub-lications,2000.
[20]吴渝生,杨文鹏,郑用琏.3个玉米杂交种和亲本SSR指纹图谱的构建[J].作物学报,2003,29(4):496-500.
[21]钱荷英.蚕品种选育及用微卫星标记(SSR)构建家蚕指纹图谱[D].北京:中国农业科学院,2005.
[22]何华勤,贾小丽,梁义元,等.RAPD和ISSR标记对水稻化感种质资源遗传多态性的分析[J].遗传学报,2004,31(9):888-894.
[2]杨昌润.美丽富饶的黔东南[J].贵州文史丛刊,1994(1):42-43.
[3]程良炳.地方稻种——禾的特点及其利用意见[J].贵州农业科学,1981(2):23-27.
[4]张再兴.贵州稻种资源[A].见:应存山.中国稻种资源[M].北京:中国农业出版社,1993.201-222.
[5]倪克鱼,孙灿慧.中国稀有稻类贵州禾的光亲和性及评价[J].贵州农业科学,2000,28(3):3-5.
[6]阮仁超,杨宗烺,陈龙,等.糯稻良种农虎禾在贵州立体农业生态条件下适应性初探[J].贵州农业科学,1992(5):25-29.
[7]肖小余,王玉平,张建勇,等.四川省主要杂交稻亲本的SSR多态性分析和指纹图谱的构建与应用[J].中国水稻科学,2006,20(1):1-7.
[8]赵波,金文林,濮绍京,等.基于SSR小豆新品种(系)DNA指纹图谱构建及亲缘分类初探[J].北京农学院学报,2008(3):1-6.
[9]于永红,李云海,马荣荣,等.用微卫星DNA标记建立宁2A的指纹图谱[J].中国水稻科学,2001,15(3):215-217.
[10]王笑一,于拴仓,张凤兰,等.小白菜品种的SSR指纹图谱及遗传特异性分析[J].华北农学报,2008,23(5):97-103.
[11]Hamwieh A,Udupa S M,Choumane W,et al.A genetic linkagemap of Lens sp.based on microsatellite and AFLP markers and thelocalization of Fusarium vascular Wilt resistance[J].Theor ApplGenet,2005,110:669-677.
[12]黎裕,贾继增,王天宇.分子标记的种类及其发展[J].生物技术通报,1999,15(4):19-22.
[13]张献龙,唐克轩.植物生物技术[M],北京:科学出版社,2004.401-500.
[14]Dellaporta S L,Wood J,Hicks J B.A plant DNA mini preparation:Version II[J].Plant Mol Biol,1983(1):19-21.
[15]Doyle J J,Doyle J L.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:149-151.
[16]宗绪晓,D Vaughan,A Kaga,等.AFLP分析小豆种(Vigna angu-laris)内遗传多样性[J].作物学报,2003,29(4):562-568.
[17]Liu KJ,Muse S V.PowerMarker:Integrated analysis environment forgenetic marker data[J].Bioinf ormatics,2005,21:2121-2129.
[18]Smith J S C,Chin E C L,Shu H,et al.An evaluation of the utilityof SSR loci as molecular markers in maize(Zea maysL.):Compari-son with data fromRFLPs and pedigree[J].Theor Appl Genet,1997,95:163-173.
[19]Rohlf F J.NTSYS-pc:Numerical taxonomy and multivariate analysissystem,version 2.1.Exeter Publications[M].New York:Exeter Pub-lications,2000.
[20]吴渝生,杨文鹏,郑用琏.3个玉米杂交种和亲本SSR指纹图谱的构建[J].作物学报,2003,29(4):496-500.
[21]钱荷英.蚕品种选育及用微卫星标记(SSR)构建家蚕指纹图谱[D].北京:中国农业科学院,2005.
[22]何华勤,贾小丽,梁义元,等.RAPD和ISSR标记对水稻化感种质资源遗传多态性的分析[J].遗传学报,2004,31(9):888-894.
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