贵州马铃薯推广品种SSR分子标记及遗传多样性分析
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摘要
为了鉴定马铃薯品种的亲缘关系,采用10对SSR分子标记引物,对19份贵州马铃薯生产品种进行SSR分子标记及遗传多样性分析。结果表明:10对SSR引物中5对获得60条清晰条带,其中49条为多态性条带,多态性比率为81.67%,平均每个引物的多态性条带数为9.80条。19份马铃薯品种的遗传相似性系数为0.43~1.00。经聚类分析,在遗传相似系数为0.56处全部参试材料聚在一起,在遗传相似系数为0.63处可明显聚为3个类群。第Ⅰ类群包括11份材料,第Ⅱ类群包括7份材料,第Ⅲ类群只有1个材料。在相似性系数0.65处,57.89%的参加品种聚在一起,表明参试品种的遗传背景较为狭窄。
In order to identify the genetic relationship of potato varieties,the 19 potato production varieties in Cuizhou were used to analyze the genetic diversity by 10 SSR molecular marker primers.The results showed that only 5 of the 10 SSR primers were obtained clear and readable bands by the PCR amplification.The 60 DNA bands were obtained,including 49 polymorphic bands with a polymorphism rate of81.67%,number of polymorphic bands per primer was 9.80.The genetic similarity coefficients of the 19 potato varieties were from 0.43 to 1.00.UPGMA cluster analysis showed that all tested varieties were got together in similarity coefficient 0.56.They were divided obviously into three groups in the genetic similarity coefficient 0.63.The group Ⅰ included 11 varieties,the group Ⅱ included 7 varieties,and group Ⅲonly one variety.There were 57.89%of the tested varieties still gathering together in the similarity coefficient 0.65.It meant that the genetic background of all tested varieties is relatively narrow.
In order to identify the genetic relationship of potato varieties,the 19 potato production varieties in Cuizhou were used to analyze the genetic diversity by 10 SSR molecular marker primers.The results showed that only 5 of the 10 SSR primers were obtained clear and readable bands by the PCR amplification.The 60 DNA bands were obtained,including 49 polymorphic bands with a polymorphism rate of81.67%,number of polymorphic bands per primer was 9.80.The genetic similarity coefficients of the 19 potato varieties were from 0.43 to 1.00.UPGMA cluster analysis showed that all tested varieties were got together in similarity coefficient 0.56.They were divided obviously into three groups in the genetic similarity coefficient 0.63.The group Ⅰ included 11 varieties,the group Ⅱ included 7 varieties,and group Ⅲonly one variety.There were 57.89%of the tested varieties still gathering together in the similarity coefficient 0.65.It meant that the genetic background of all tested varieties is relatively narrow.
引文
[1]Norero N,Malleville J,Huarte M,et al.Cost efficient potato(Solarium tuberosum L)cultivar identification by microsatellite amplification[J].Potato Researeh,2002,45:131-138.
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[3]段艳风,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
[4]李先平,王冬冬,陈秀华,等.SSR分子标记分析彩色马铃薯品种间的遗传关系[J].东北农业大学学报,2012,43(7):61-69.
[5]石景,宋波涛,金开建.SSR标记的彩色马铃薯遗传多样性分析及指纹图谱构建[J].农业生物技术学报,2012,20(4):362-371.
[6]唐铭霞,何卫,胡建军,等.四川省马铃薯主栽品种的遗传多样性分析[J].西南农业学报,2010,23(6):1805-1808.
[7]张自强,于肖夏,鞠天华,等.36个马铃薯品种的SSR分析[J].华北农学报,2012,27(1):93-97.
[8]甘晓燕,程永芳,巩檑,等.SRAP和SSR标记对马铃薯遗传多样性的差异分析[J].中国农学通报,2015(30):225-230.
[9]黄团,李旭,黄先群,等.紫色马铃薯辐射处理表型及DNA变异鉴定[J].西北农业学报,2014,26(3):145-150.
[10]谢春梅,林蓉,孙雁,等.应用SSR标记技术鉴定马铃薯杂交实生种子的纯度[J].种子,2008,27(8):7-9.
[11]于卓,郝治满,鞠天华,等.马铃薯‘H-027'与‘陇薯6号'和‘陇薯7号'杂种优良单株的SSR分析[J].中国园艺文摘,2015(1):8-11.
[12]黄团,邓宽平,雷尊国,等.马铃薯~(60)Co-γ辐射诱变突变体的分子鉴定[J].贵州农业科学,2010,38(8):8-IO.
[13]王绍鹏,邱彩玲,李勇,等.马铃薯DNA提取及SSR标记分析[J].东北农业大学学报,2015,加(5):7-12.
[14]李飞,邓宽平,段艳凤,等.8个马铃薯新品种(系)的遗传分析和指纹图谱构建[J].贵州农业科学,2010,38(9):9-10.
[15]李丽,黄先群,何天久,等.贵州马铃薯审定品种及区试材料的SSR遗传多样性分析[J].西南农业学报,2013,26(3):909-913.
[16]黄先群,冉江,黄团,等.马铃薯引进品种及变异材料SSR遗传多样性分析[J].西南农业学报,2014,27(4):1399-1403.
[2]Moisan-Thiery M,Marfaadour S,Kerlan M C,et al.Potato cultivar identification using simple sequence repeats markers(SSR)[J].Potato Research,2005,48:191-200.
[3]段艳风,刘杰,卞春松,等.中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J].作物学报,2009,35(8):1451-1457.
[4]李先平,王冬冬,陈秀华,等.SSR分子标记分析彩色马铃薯品种间的遗传关系[J].东北农业大学学报,2012,43(7):61-69.
[5]石景,宋波涛,金开建.SSR标记的彩色马铃薯遗传多样性分析及指纹图谱构建[J].农业生物技术学报,2012,20(4):362-371.
[6]唐铭霞,何卫,胡建军,等.四川省马铃薯主栽品种的遗传多样性分析[J].西南农业学报,2010,23(6):1805-1808.
[7]张自强,于肖夏,鞠天华,等.36个马铃薯品种的SSR分析[J].华北农学报,2012,27(1):93-97.
[8]甘晓燕,程永芳,巩檑,等.SRAP和SSR标记对马铃薯遗传多样性的差异分析[J].中国农学通报,2015(30):225-230.
[9]黄团,李旭,黄先群,等.紫色马铃薯辐射处理表型及DNA变异鉴定[J].西北农业学报,2014,26(3):145-150.
[10]谢春梅,林蓉,孙雁,等.应用SSR标记技术鉴定马铃薯杂交实生种子的纯度[J].种子,2008,27(8):7-9.
[11]于卓,郝治满,鞠天华,等.马铃薯‘H-027'与‘陇薯6号'和‘陇薯7号'杂种优良单株的SSR分析[J].中国园艺文摘,2015(1):8-11.
[12]黄团,邓宽平,雷尊国,等.马铃薯~(60)Co-γ辐射诱变突变体的分子鉴定[J].贵州农业科学,2010,38(8):8-IO.
[13]王绍鹏,邱彩玲,李勇,等.马铃薯DNA提取及SSR标记分析[J].东北农业大学学报,2015,加(5):7-12.
[14]李飞,邓宽平,段艳凤,等.8个马铃薯新品种(系)的遗传分析和指纹图谱构建[J].贵州农业科学,2010,38(9):9-10.
[15]李丽,黄先群,何天久,等.贵州马铃薯审定品种及区试材料的SSR遗传多样性分析[J].西南农业学报,2013,26(3):909-913.
[16]黄先群,冉江,黄团,等.马铃薯引进品种及变异材料SSR遗传多样性分析[J].西南农业学报,2014,27(4):1399-1403.
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