SSR标记在江苏粳稻品种鉴定中的应用研究
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摘要
利用农业行业标准"NY/T-1433—2007、NY/T-1433—2014"中分别推荐的24和48个SSR标记,对2013年江苏区试粳稻品系和2009~2014年间江苏审定品种中的46个粳稻品种进行鉴定,证实2个版本中的SSR标记均不能完全鉴别供试的江苏粳稻品种。以12个代表性粳稻品种为材料,从714个SSR标记中筛选到133个在代表性品种间存在多态性的标记,结合"NY/T-1433—2014"中的多态性标记共计153个,构建了46个粳稻品种的SSR指纹。以品种间SSR指纹差异位点数、标记分布均匀性及多态性评价指标值为筛选依据,从153个SSR标记中初步确定了52个核心标记用于江苏水稻品种鉴定,构建了46个粳稻品种的SSR指纹。对52个标记在江苏水稻尤其是粳稻品种鉴定中的优点及进一步完善的策略进行了讨论。
The sets of 24 and 48 SSR(Simple sequence repeat) markers for rice variety identification reported in Industrial standards of Agriculture Ministry, "NY/T-1433-2007"(named 2007 version) and "NY/T-1433-2014"(named2014 version),respectively,were used to identify 46 japonica rice varieties authorized by Jiangsu Government from 2009 to 2014.Results indicated that both 24 and 48 markers were not able to completely identify all 46 varieties.A total of 133 out of 714 SSR markers showed polymorphism on 12-set japonica varieties selected were obtained.Plus the polymorphic SSR markers from the Industrial standard of 2014 version,total of 153 polymorphic SSR markers were used to develop the DNA fingerprints of the 46 japonica varieties.According to the filter criteria that any of two varieties have at least2 differential SSR loci and the distribution uniformity of the markers on rice genome and the PIC(polymorphic information content) of each marker,we preliminarily determined a set of 52 core SSR markers that were capable of identifying rice varieties in Jiangsu.The specific fingerprint for each of the 46 rice varieties was established by using the 52 core SSR markers.The advantages and the further improving strategies of the 52 SSR markers on identifying Jiangsu rice varieties especially on japonica rice varieties were discussed.
The sets of 24 and 48 SSR(Simple sequence repeat) markers for rice variety identification reported in Industrial standards of Agriculture Ministry, "NY/T-1433-2007"(named 2007 version) and "NY/T-1433-2014"(named2014 version),respectively,were used to identify 46 japonica rice varieties authorized by Jiangsu Government from 2009 to 2014.Results indicated that both 24 and 48 markers were not able to completely identify all 46 varieties.A total of 133 out of 714 SSR markers showed polymorphism on 12-set japonica varieties selected were obtained.Plus the polymorphic SSR markers from the Industrial standard of 2014 version,total of 153 polymorphic SSR markers were used to develop the DNA fingerprints of the 46 japonica varieties.According to the filter criteria that any of two varieties have at least2 differential SSR loci and the distribution uniformity of the markers on rice genome and the PIC(polymorphic information content) of each marker,we preliminarily determined a set of 52 core SSR markers that were capable of identifying rice varieties in Jiangsu.The specific fingerprint for each of the 46 rice varieties was established by using the 52 core SSR markers.The advantages and the further improving strategies of the 52 SSR markers on identifying Jiangsu rice varieties especially on japonica rice varieties were discussed.
引文
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[15]NY/T 1433—2014:水稻品种鉴定技术规程SSR标记法[S].
[16]Porebski S,Bailey L G,Baum B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Molecular Biology Reporter,1997,15(1);8-15.
[17]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].Am J Hum Genet,1980,32(3):314-331.
[2]李晓辉,李新海,张世煌.植物新品种保护与DUS测试技术[J].中国农业科学,2003,36(11):1419-1422.
[3]Singh R K,Sharma R K,Singh A K,et al.Suitability of mapped sequence tagged microsateUite site markers for establishing distinctness,uniformity and stability in aromatic rice[J].Euphytica,2004,135(2):135-143.
[4]Garland S H,Lewin L,Abedinia M,et al.The use of microsateUite polymorphisms for the identification of Australian breeding lines of rice(Oryza sativa L.)[J].Euphytica,1999,108(1):53-63.
[5]周治华.加强品种管理保障良种供应[J].种子科技,2011(1):9-10.
[6]李莉,杨剑波,汪秀峰,等.利用RAPD和微卫星标记对协优杂交稻及其亲本进行区别与鉴定[J].中国水稻科学,2000,14(4):203-207.
[7]Roder M S,Wendehake K,Korzun V,et al.Construction and analysis of a microsatellite-based database of European wheat varieties[J].Theor Appl Genet,2002,106(1):67-73.
[8]马鹏.糯玉米自交系遗传多样性分析[D].天津:天津农学院,2014.
[9]殷兆晴.水稻区域试验品种DNA指纹图谱构建及亲本材料间遗传差异的分析[D].雅安:四川农业大学,2006.
[10]刘殊,程慧,王飞,等.我国杂交水稻主要恢复系的DNA多态性研究[J].中国水稻科学,2002,1 6(1):1-5.
[11]何光华,裴炎,杨光伟,等.我国中籼杂交稻亲本的DNA变异性研究[J].作物学报,2000,26(4):449-454.
[12]段世华,毛加宁,朱英国.用微卫星DNA标记对我国杂交水稻主要恢复系遗传差异的检测分析[J].遗传学报,2002.29(3):250-254.
[13]李云海,肖晗,张春庆,等.用微卫星DNA标记检测中国主要杂交水稻亲本的遗传差异[J].植物学报,1999,41(10):1061-1066.
[14]NY/T 1433—2007:水稻品种鉴定DNA指纹方法[S].
[15]NY/T 1433—2014:水稻品种鉴定技术规程SSR标记法[S].
[16]Porebski S,Bailey L G,Baum B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Molecular Biology Reporter,1997,15(1);8-15.
[17]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].Am J Hum Genet,1980,32(3):314-331.
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