利用籼粳稻特异InDel标记分析云南糯稻品种的籼粳特性
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摘要
利用籼粳杂种优势是实现水稻超高产育种目标的重要途径之一。利用根据粳稻日本晴和籼稻93-11基因组序列设计的34对特异性插入/缺失(Insertion/Deletion,InDel)引物,对采自云南省7个地州17个县的181份糯稻品种进行籼粳性分析。通过对这些糯稻品种DNA样品在这34个InDel位点上的籼型或粳型基因频率,确定各个糯稻品种的籼、粳性。结果表明:141份测试品种被鉴定为典型籼稻,18份为籼稻,8份为典型粳稻,13份为粳稻,1份为偏粳稻。主成分分析表明,云南糯稻籼粳分化明显。
Utilization of Indica-Japonica heterosis was one of the most important ways to achieve the goal of high yield rice breeding.Indica-Japonica classifications of 181 glutinous rice varieties collected in 17 counties of 7 cities in Yunnan were analyzed by calculating Indica and Japonica gene frequencies on 34 InDel sites of each glutinous rice varieties DNA samples and principal component analysis(PCA).181 Yunnan glutinous rice varieties were classified as 141 typical Indica rice,18 Indica rice,1 Japonicaclinous rice,13 Japonica rice and 8 typical Japonica rice.PCA showed that differentiations of Yunnan glutinous rice were significant.
Utilization of Indica-Japonica heterosis was one of the most important ways to achieve the goal of high yield rice breeding.Indica-Japonica classifications of 181 glutinous rice varieties collected in 17 counties of 7 cities in Yunnan were analyzed by calculating Indica and Japonica gene frequencies on 34 InDel sites of each glutinous rice varieties DNA samples and principal component analysis(PCA).181 Yunnan glutinous rice varieties were classified as 141 typical Indica rice,18 Indica rice,1 Japonicaclinous rice,13 Japonica rice and 8 typical Japonica rice.PCA showed that differentiations of Yunnan glutinous rice were significant.
引文
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[18]GOLOMB L,The origin,spread and persistence of glu-tinous rice as a staple crop in Mainland Southeast Asia[J].Journal of Southeast Asian Studies,1976,7(1):1-15.
[19]OLSEN K M,PURUGGANAN M D.Molecular evi-dence on the origin and evolution of glutinous rice[J].Genetics,2002,162(2):941-950.
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[21]游修龄.糯稻文化区[J].中国稻米,1995,2(1):26-28.
[22]游修龄.糯米饮食文化圈的兴衰[J].饮食文化研究,2006,(3):3-9.
[23]叶昌荣,王莉花,徐福荣.云南作物遗传资源研究现状及设想[J].植物遗传资源科学,2001,2(3):60-62.
[24]SINGHR K.Genetic resource and the role of interna-tional collaboration in rice breeding[J].Genome,1999,42(4):635-641.
[25]MURRAY M G,THOMPSON W F.Rapid isolation ofhigh molecular weight plant DNA[J].Nucleic AcidsResearch,1980,8(19):4321-4325.
[26]赵一之,李素英,曹瑞,等.脓疮草复合体的形态性状分析与分类修订[J].植物分类学报,1998,36(3):193-205.
[27]张桂权,杨进昌.栽培稻杂种不育性的遗传研究:Ⅲ.不同类型品种F1花粉不育性的等位基因分化[J].遗传学报,1993,20(6):541-551.
[28]GLASZMANN J C.Isozymes and classification of Asianrice varieties[J].Theoretical and Applied Genetics,1987,74(1):21-30.
[29]BLAIR MW,PANAUD O,MCCOUCHS R.Inter-sim-ple sequence repeat(ISSR)amplification for analysis ofmicrosatellite motif frequency and fingerprinting in rice(Oryza sativaL.)[J].Theoretical and Applied Ge-netics,1999,98(5):780-782.
[2]LU B R,ZHENG K L,QIAN HR,et al..Genetic dif-ferentiation of wild relatives of rice as assessed by theRFLP analysis[J].Theoretical and Applied Genetics,2002,106(1):101-106.
[3]OKA HI.Origin of cultivated rice[M].Tokyo:JapanScientific Societies Press,1988.
[4]刘克德,张启发,张端品,等.云南地方稻种的遗传变异和籼粳分化[J].植物学报,1995,37(9):718-724.
[5]MORISHIMA H,OKA H I.Phylogenetic differentiationof cultivated rice.ⅩⅫ.Numerable evaluation of theIndica-Japonica differentiation[J].Japanese Journal ofBreeding,1981,31(4):402-413.
[6]邰丽梅,杜娟,曾亚文,等.思茅地区核心稻种籼粳特异性标记的聚类分析[J].西南农业学报,2006,19(B09):49-53.
[7]王象坤,李任华,孙传清,等.亚洲栽培稻的亚种及亚种间杂交稻的认定与分类[J].科学通报,1997,42(24):2596-2603.
[8]卢宝荣,蔡星星,金鑫.籼稻和粳稻的高效分子鉴定方法及其在水稻育种和进化研究中的意义[J].自然科学进展,2009,19(5):26-36.
[9]蔡星星,刘晶,仇吟秋,等.籼稻93-11和粳稻日本晴DNA插入缺失差异片段揭示的水稻籼-粳分化[J].复旦学报:自然科学版,2006,45(3):309-315.
[10]KHUSH G.Green revolution:the way forward[J].Nature Reviews Genetics,2001,2:815-822.
[11]PENG S B,LAZA R C,VISPERAS R M,et al..Rice:Progress in Breaking the Yield Ceiling[C/CD]//“Newdirection for a diverse planet”.Proceed-ings of the 4th International Crop Science Congress.Brisbane,Australia:Published on CDROM,2004.
[12]姜健,李金泉,徐正进.水稻籼粳杂交育种研究进展[J].吉林农业科学,2003,28(1):9-14.
[13]朱作峰,孙传清,李自超,等.用SSR标记对水稻品种的分类研究[J].农业生物技术学报,2001,9(1):58-61.
[14]姜树坤,钟鸣,徐正进,等.基于RAPD标记进行水稻籼粳分类研究[J].沈阳农业大学学报,2006,37(4):639-641.
[15]程侃声,王象坤,卢义宣,等.云南稻种资源的综合研究与利用:II.亚洲栽培稻分类的再认识[J].作物学报,1984,10(4):271-280.
[16]程侃声.亚洲稻籼粳亚种的鉴别[M].昆明:云南科技出版社,1993.
[17]杨慧,陆春明,贾亦飞,等.云南糯稻遗传多样性的SSR分析[J].分子植物育种,2008,6(6):1068-1074.
[18]GOLOMB L,The origin,spread and persistence of glu-tinous rice as a staple crop in Mainland Southeast Asia[J].Journal of Southeast Asian Studies,1976,7(1):1-15.
[19]OLSEN K M,PURUGGANAN M D.Molecular evi-dence on the origin and evolution of glutinous rice[J].Genetics,2002,162(2):941-950.
[20]RODER W,KEOBOULAPHA B,VANNALATH K,etal..Glutinous rice and its importance for hill farmers inLaos[J].Economic Botany,1996,50(4):401-408.
[21]游修龄.糯稻文化区[J].中国稻米,1995,2(1):26-28.
[22]游修龄.糯米饮食文化圈的兴衰[J].饮食文化研究,2006,(3):3-9.
[23]叶昌荣,王莉花,徐福荣.云南作物遗传资源研究现状及设想[J].植物遗传资源科学,2001,2(3):60-62.
[24]SINGHR K.Genetic resource and the role of interna-tional collaboration in rice breeding[J].Genome,1999,42(4):635-641.
[25]MURRAY M G,THOMPSON W F.Rapid isolation ofhigh molecular weight plant DNA[J].Nucleic AcidsResearch,1980,8(19):4321-4325.
[26]赵一之,李素英,曹瑞,等.脓疮草复合体的形态性状分析与分类修订[J].植物分类学报,1998,36(3):193-205.
[27]张桂权,杨进昌.栽培稻杂种不育性的遗传研究:Ⅲ.不同类型品种F1花粉不育性的等位基因分化[J].遗传学报,1993,20(6):541-551.
[28]GLASZMANN J C.Isozymes and classification of Asianrice varieties[J].Theoretical and Applied Genetics,1987,74(1):21-30.
[29]BLAIR MW,PANAUD O,MCCOUCHS R.Inter-sim-ple sequence repeat(ISSR)amplification for analysis ofmicrosatellite motif frequency and fingerprinting in rice(Oryza sativaL.)[J].Theoretical and Applied Ge-netics,1999,98(5):780-782.
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