上海“本地人”正常人群GSTT1和GSTM1基因型多态性研究
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摘要
目的与方法 :以聚合酶链式反应 (PCR)技术对上海“本地人”正常人群GSTT1和GSTM1基因型多态性进行了研究。 结果 :上海“本地人”正常人群中GSTT1纯合缺损基因型和GSTM 1纯合缺损基因型的比率分别为 48 5 3 %和 5 4 40 % ,与上海市区正常人群中两种基因型的分布比率 (分别为 49 32 %和 48 86 % )没有显著差别。GSTT1-GSTM 1综合基因型 4种状态在两个人群中的分布频率也没有显著差别。结论 :GSTT1和GSTM1基因型多态性在同为上海地区居民的上海“本地人”正常人群和上海市区正常人群中呈均一分布。
Purpose and Methods: Population polymorphisms of the GSTT1 and M1 genes in the Shanghai “indi gene” group were evaluated using the PCR procedure. Results: In this group, the frequencies of GSTT1 null and GSTM1 null genotype are 48.53% and 54.40%, respectively. No significant differences were detected between urban individuals (49.32% and 48.86%, respectively) and an “indigene” group in suburb of Shanghai for GSTT1 and GSTM1 genotypes alone, as well as for four combined genotypes of GSTT1 and GSTM1. Conclusion: Between the “indigene” population in remote suburb and the urban population in downtown of Shanghai the genetic polymorphism distribution of GSTT1 and GSTM1 genes is proved to be homogenous.
Purpose and Methods: Population polymorphisms of the GSTT1 and M1 genes in the Shanghai “indi gene” group were evaluated using the PCR procedure. Results: In this group, the frequencies of GSTT1 null and GSTM1 null genotype are 48.53% and 54.40%, respectively. No significant differences were detected between urban individuals (49.32% and 48.86%, respectively) and an “indigene” group in suburb of Shanghai for GSTT1 and GSTM1 genotypes alone, as well as for four combined genotypes of GSTT1 and GSTM1. Conclusion: Between the “indigene” population in remote suburb and the urban population in downtown of Shanghai the genetic polymorphism distribution of GSTT1 and GSTM1 genes is proved to be homogenous.
引文
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[2 ] 林国芳 ,谭靖伟 ,沈建华 上海正常人群中谷胱甘肽S -转移酶T1纯合缺损 [J] .癌变·畸变·突变 ,1998,10 (6 ) :345~ 348.
[3] 马晴雯 ,林国芳 ,陈纪刚 ,等 上海“本地人”正常人群hGSTP1基因A1 5 78G多态性研究 [J] .癌变·畸变·突变 ,2 0 0 0 ,12 (1) :6~8
[4] 马晴雯 ,沈建华 ,林国芳 外源物代谢有关基因的多态性问题 :昨天、今天和明天 [J] 中华劳动卫生职业病杂志 ,1999,17:318~ 32 0
[5 ] LeeEJD ,WongJYY ,YoohPN ,etal.GlutathioneS -trans ferase-θ(GSTT1)geneticpolymorphismamongChineseMalaysandIndiansinSingapore[J] .Pharmacogenetics,1995 ,5 :332~334.
[6 ] ZhaoB ,LeeEJD ,WongJYY ,etal.FrequencyofmutantCYP1A1,NAT2 ,andGSTM 1allelesinnormalIndianandMalays[J] .Pharmacogenetics,1995 ,5 :2 75~ 2 80 .
[2 ] 林国芳 ,谭靖伟 ,沈建华 上海正常人群中谷胱甘肽S -转移酶T1纯合缺损 [J] .癌变·畸变·突变 ,1998,10 (6 ) :345~ 348.
[3] 马晴雯 ,林国芳 ,陈纪刚 ,等 上海“本地人”正常人群hGSTP1基因A1 5 78G多态性研究 [J] .癌变·畸变·突变 ,2 0 0 0 ,12 (1) :6~8
[4] 马晴雯 ,沈建华 ,林国芳 外源物代谢有关基因的多态性问题 :昨天、今天和明天 [J] 中华劳动卫生职业病杂志 ,1999,17:318~ 32 0
[5 ] LeeEJD ,WongJYY ,YoohPN ,etal.GlutathioneS -trans ferase-θ(GSTT1)geneticpolymorphismamongChineseMalaysandIndiansinSingapore[J] .Pharmacogenetics,1995 ,5 :332~334.
[6 ] ZhaoB ,LeeEJD ,WongJYY ,etal.FrequencyofmutantCYP1A1,NAT2 ,andGSTM 1allelesinnormalIndianandMalays[J] .Pharmacogenetics,1995 ,5 :2 75~ 2 80 .
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